1887

Abstract

SR protein ASF/SF2 is a general pre-mRNA splicing factor as well as a regulator of alternative splicing. Data presented here show that ASF/SF2 is efficiently recruited to sites in the nucleus where adenovirus genes are transcribed and the resulting pre-mRNAs are processed. At the intermediate stages of a productive infection, ASF/SF2 colocalizes with small nuclear ribonucleoprotein particles (snRNPs), splicing factors in ring-like structures surrounding viral replication centres and, at late stages of the infection, in enlarged speckles. Results presented here demonstrate that ASF/SF2 requires only one of the two RNA-recognition motifs (RRMs) present in the protein for its efficient recruitment to the ring-like structures, where viral pre-mRNAs are transcribed and processed, and that the arginine/serine-rich (RS) domain in ASF/SF2 is both redundant and insufficient for the translocation of the protein to active viral RNA polymerase II genes in adenovirus-infected cells.

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2004-03-01
2020-10-22
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References

  1. Bridge E., Carmo-Fonseca M., Lamond A., Pettersson U. 1993; Nuclear organization of splicing small nuclear ribonucleoproteins in adenovirus-infected cells. J Virol 67:5792–5802
    [Google Scholar]
  2. Bridge E., Xia D. X., Carmo-Fonseca M., Cardinali B., Lamond A. I., Pettersson U. 1995; Dynamic organization of splicing factors in adenovirus-infected cells. J Virol 69:281–290
    [Google Scholar]
  3. Bridge E., Riedel K. U., Johansson B. M., Pettersson U. 1996; Spliced exons of adenovirus late RNAs colocalize with snRNP in a specific nuclear domain. J Cell Biol 135:303–314
    [Google Scholar]
  4. Caceres J. F., Krainer A. R. 1993; Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains. EMBO J 12:4715–4726
    [Google Scholar]
  5. Caceres J. F., Screaton G. R., Krainer A. R. 1998; A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm. Genes Dev 12:55–66
    [Google Scholar]
  6. Dye B. T., Patton J. G. 2001; An RNA recognition motif (RRM) is required for the localization of PTB-associated splicing factor (PSF) to subnuclear speckles. Exp Cell Res 263:131–144
    [Google Scholar]
  7. Fox A. H., Lam Y. W., Leung A. K. L., Lyon C. E., Andersen J., Mann M., Lamond A. 2002; Paraspeckles: a novel nuclear domain. Curr Biol 12:13–25
    [Google Scholar]
  8. Gama-Carvalho M., Krauss R. D., Chiang L., Valcarcel J., Green M. R., Carmo-Fonseca M. 1997; Targeting of U2AF65 to sites of active splicing in the nucleus. J Cell Biol 137:975–987
    [Google Scholar]
  9. Graveley B. R. 2000; Sorting out the complexity of SR protein functions. RNA 6:1197–1211
    [Google Scholar]
  10. Hanamura A., Caceres J. F., Mayeda A., Franza B. R. Jr, Krainer A. R. 1998; Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors. RNA 4:430–444
    [Google Scholar]
  11. Linne T., Jornvall H., Philipson L. 1977; Purification and characterization of the phosphorylated DNA-binding protein from adenovirus-type-2-infected cells. Eur J Biochem 76:481–490
    [Google Scholar]
  12. Misteli T., Caceres J. F., Spector D. L. 1997; The dynamics of a pre-mRNA splicing factor in living cells. Nature 387:523–527
    [Google Scholar]
  13. Pombo A., Ferreira J., Bridge E., Carmo-Fonseca M. 1994; Adenovirus replication and transcription sites are spatially separated in the nucleus of infected cells. EMBO J 13:5075–5085
    [Google Scholar]
  14. Sleeman J., Lyon C. E., Platani M., Kreivi J. P., Lamond A. I. 1998; Dynamic interactions between splicing snRNPs, coiled bodies and nucleoli revealed using snRNP protein fusions to the green fluorescent protein. Exp Cell Res 243:290–304
    [Google Scholar]
  15. Valcarcel J., Gaur R. K., Singh R., Green M. R. 1996; Interaction of U2AF65 RS region with pre-mRNA branch point and promotion of base pairing with U2 snRNA [corrected]. Science 273:1706–1709
    [Google Scholar]
  16. Zuo P., Manley J. L. 1993; Functional domains of the human splicing factor ASF/SF2. EMBO J 12:4727–4737
    [Google Scholar]
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