1887

Abstract

The biological characteristics of human T-cell leukaemia virus type I (HTLV-I) are not yet well understood. UV light C (UV-C) sensitivity of HTLV-I was studied using a newly established infectivity assay: infection with cell-free HTLV-I dose-dependently induced syncytial plaques in cat cells transduced with the gene of HTLV-I. HTLV-I was inactivated by a much lower UV dose than bovine leukaemia virus (BLV). The D (10 % survival dose) of HTLV-I was about 20 J m, while that of BLV was about 180 J m, which was similar to the reported D of BLV. The UV sensitivity of HTLV-I and BLV was also examined by detecting viral DNA synthesis 24 h after infection. The D values determined by PCR using the primers for HTLV-I and BLV were close to those determined by the infectivity assays. Further PCR analyses were then performed to determine D values using several different primers located between the 5′-long terminal repeat (5′-LTR) and the gene. The difference in UV sensitivity between HTLV-I and BLV was detected very early during replication, even during reverse transcription of the 5′-LTR of irradiated viruses, and became more prominent as reverse transcription proceeded towards the gene. Chimeric mouse retroviruses that contain the LTR- fragments of HTLV-I and BLV were made and hardly any difference in UV sensitivity was detected between them, suggesting that the difference was not determined by the linear RNA sequences of HTLV-I and BLV. HTLV-I was found to be much more sensitive than other retroviruses to UV.

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2004-08-01
2020-10-29
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