1887

Abstract

The movement protein (MP) of (CPMV) forms tubules through plasmodesmata in infected plants thus enabling virus particles to move from cell to cell. Localization studies of mutant MPs fused to GFP in protoplasts and plants identified several functional domains within the MP that are involved in distinct steps during tubule formation. Coinoculation experiments and the observation that one of the C-terminal deletion mutants accumulated uniformly in the plasma membrane suggest that dimeric or multimeric MP is first targeted to the plasma membrane. At the plasma membrane the MP quickly accumulates in peripheral punctuate spots, from which tubule formation is initiated. One of the mutant MPs formed tubules containing virus particles on protoplasts, but could not support cell-to-cell movement in plants. The observations that this mutant MP accumulated to a higher level in the cell than wt MP and did not accumulate in the cell wall opposite infected cells suggest that breakdown or disassembly of tubules in neighbouring, uninfected cells is required for cell-to-cell movement.

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2003-12-01
2019-10-22
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References

  1. Bassi, M., Favali, M. A. & Conti, G. G. ( 1974; ). Cell wall protrusions induced by cauliflower mosaic virus in Chinese cabbage leaves: a cytochemical and autoradiographic study. Virology 60, 353–358.[CrossRef]
    [Google Scholar]
  2. Bertens, P., Wellink, J., Goldbach, R. & van Kammen, A. ( 2000; ). Mutational analysis of the cowpea mosaic virus movement protein. Virology 267, 199–208.[CrossRef]
    [Google Scholar]
  3. Bertens, P., Heijne, W., van der Wel, N., Wellink, J. & van Kammen, A. ( 2003; ). Studies on the C-terminus of the Cowpea mosaic virus movement protein. Arch Virol 148, 265–279.[CrossRef]
    [Google Scholar]
  4. Carvalho, C. M., Wellink, J., Ribeiro, S. G., Goldbach, R. W. & van Lent, J. W. M. ( 2003; ). The C-terminal region of the movement protein of Cowpea mosaic virus is involved in binding to the large but not to the small coat protein. J Gen Virol 84, 2271–2277.[CrossRef]
    [Google Scholar]
  5. Dean, A. M., & Koshland, D. E., Jr ( 1990; ). Electrostatic and steric contributions to regulation at the active site of isocitrate dehydrogenase. Science 249, 1044–1046.[CrossRef]
    [Google Scholar]
  6. Gopinath, K., Wellink, J., Porta, C., Taylor, K. M., Lomonossoff, G. P. & van Kammen, A. ( 2000; ). Engineering cowpea mosaic virus RNA-2 into a vector to express heterologous proteins in plants. Virology 267, 159–173.[CrossRef]
    [Google Scholar]
  7. Gopinath, K., Bertens, P., Pouwels, J., Marks, H., van Lent, J., Wellink, J. & van Kammen, A. ( 2003; ). Intracellular distribution of cowpea mosaic virus movement protein as visualized by green fluorescent protein fusions. Arch Virol (in press).
    [Google Scholar]
  8. Ho, S. N., Hunt, H. D., Horton, R. M., Pullen, J. K. & Pease, L. R. ( 1989; ). Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51–59.[CrossRef]
    [Google Scholar]
  9. Huang, Z., Han, Y. & Howell, S. H. ( 2000; ). Formation of surface tubules and fluorescent foci in Arabidopsis thaliana protoplasts expressing a fusion between the green fluorescent protein and the cauliflower mosaic virus movement protein. Virology 271, 58–64.[CrossRef]
    [Google Scholar]
  10. Huang, Z., Han, Y. & Howell, S. H. ( 2001; ). Effects of movement protein mutations on the formation of tubules in plant protoplasts expressing a fusion between the green fluorescent protein and Cauliflower mosaic virus movement protein. Mol Plant Microbe Interact 14, 1026–1031.[CrossRef]
    [Google Scholar]
  11. Kasteel, D., Wellink, J., Verver, J., van Lent, J., Goldbach, R. & van Kammen, A. ( 1993; ). The involvement of cowpea mosaic virus M RNA-encoded proteins in tubule formation. J Gen Virol 74, 1721–1724.[CrossRef]
    [Google Scholar]
  12. Kasteel, D. T., Perbal, M. C., Boyer, J. C., Wellink, J., Goldbach, R. W., Maule, A. J. & van Lent, J. W. ( 1996; ). The movement proteins of cowpea mosaic virus and cauliflower mosaic virus induce tubular structures in plant and insect cells. J Gen Virol 77, 2857–2864.[CrossRef]
    [Google Scholar]
  13. Lazarowitz, S. G. & Beachy, R. N. ( 1999; ). Viral movement proteins as probes for intracellular and intercellular trafficking in plants. Plant Cell 11, 535–548.[CrossRef]
    [Google Scholar]
  14. Lekkerkerker, A., Wellink, J., Yuan, P., van Lent, J., Goldbach, R. & van Kammen, A. B. ( 1996; ). Distinct functional domains in the cowpea mosaic virus movement protein. J Virol 70, 5658–5661.
    [Google Scholar]
  15. Linstead, P. J., Hills, G. J., Plaskitt, K. A., Wilson, I. G., Harker, C. L. & Maule, A. J. ( 1988; ). The subcellular localization of the gene I product of cauliflower mosaic virus is consistent with a function associated with virus spread. J Gen Virol 69, 1809–1818.[CrossRef]
    [Google Scholar]
  16. Packter, N. M. & Stumpf, P. K. ( 1975; ). Fat metabolism in higher plants. The effect of cerulenin on the synthesis of medium- and long-chain acids in leaf tissue. Arch Biochem Biophys 167, 655–667.[CrossRef]
    [Google Scholar]
  17. Pouwels, J., Carette, J. E., Van Lent, J. & Wellink, J. ( 2002a; ). Cowpea mosaic virus: effects on host cell processes. Mol Plant Pathol 3, 411–418.[CrossRef]
    [Google Scholar]
  18. Pouwels, J., Van Der Krogt, G. N., Van Lent, J., Bisseling, T. & Wellink, J. ( 2002b; ). The cytoskeleton and the secretory pathway are not involved in targeting the cowpea mosaic virus movement protein to the cell periphery. Virology 297, 48–56.[CrossRef]
    [Google Scholar]
  19. Sanchez-Navarro, J. A. & Bol, J. F. ( 2001; ). Role of the Alfalfa mosaic virus movement protein and coat protein in virus transport. Mol Plant Microbe Interact 14, 1051–1062.[CrossRef]
    [Google Scholar]
  20. Schneider, F., Lessire, R., Bessoule, J. J., Juguelin, H. & Cassagne, C. ( 1993; ). Effect of cerulenin on the synthesis of very-long-chain fatty acids in microsomes from leek seedlings. Biochim Biophys Acta 1152, 243–252.[CrossRef]
    [Google Scholar]
  21. Thomas, C. L. & Maule, A. J. ( 1999; ). Identification of inhibitory mutants of Cauliflower mosaic virus movement protein function after expression in insect cells. J Virol 73, 7886–7890.
    [Google Scholar]
  22. van Bokhoven, H., Verver, J., Wellink, J. & van Kammen, A. ( 1993; ). Protoplasts transiently expressing the 200K coding sequence of cowpea mosaic virus B-RNA support replication of M-RNA. J Gen Virol 74, 2233–2241.[CrossRef]
    [Google Scholar]
  23. van Lent, J. W., Wellink, J. & Goldbach, R. W. ( 1990; ). Evidence for the involvement of the 58K and 48K proteins in the intracellular movement of cowpea mosaic virus. J Gen Virol 71, 219–223.[CrossRef]
    [Google Scholar]
  24. van Lent, J., Storms, M., van der Meer, F., Wellink, J. & Goldbach, R. ( 1991; ). Tubular structures involved in movement of cowpea mosaic virus are also formed in infected cowpea protoplasts. J Gen Virol 72, 2615–2623.[CrossRef]
    [Google Scholar]
  25. Wellink, J., van Lent, J. W., Verver, J., Sijen, T., Goldbach, R. W. & van Kammen, A. ( 1993a; ). The cowpea mosaic virus M RNA-encoded 48-kilodalton protein is responsible for induction of tubular structures in protoplasts. J Virol 67, 3660–3664.
    [Google Scholar]
  26. Wellink, J., Verver, J. & van Kammen, A. ( 1993b; ). Mutational analysis of AUG codons of cowpea mosaic virus M RNA. Biochimie 75, 741–747.[CrossRef]
    [Google Scholar]
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