1887

Abstract

The complete genome sequence of a new isolate of enzootic nasal tumour virus (ENTV-2), associated with enzootic nasal adenocarcinoma (ENA) of goats, was determined. The genome exhibits a genetic organization characteristic of -retroviruses. ENTV-2 is closely related to the retrovirus (ENTV-1) associated with enzootic adenocarcinoma of sheep, and to jaagsiekte retrovirus. The main sequence differences between these viruses reside in , the U3 LTR, two small regions in and the transmembrane (TM) region of . Sequence analysis of the TM region of from several sheep and goats naturally affected by ENA suggested that ENTV-1 and ENTV-2 are distinct viruses rather than geographical variants. Although both viruses transform secretory epithelial cells of the ethmoid turbinate, the study of their tissue distribution using specific PCRs showed that ENTV-2 establishes a disseminated lymphoid infection whereas ENTV-1 is mainly confined to the tumour.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19125-0
2003-08-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/8/vir842245.html?itemId=/content/journal/jgv/10.1099/vir.0.19125-0&mimeType=html&fmt=ahah

References

  1. Alberti A., Murgia C., Liu S. L., Mura M., Cousens C., Sharp J. M., Miller A. D., Palmarini M. 2002; Envelope induced cell transformation by ovine β -retroviruses. J Virol 76:5387–5394
    [Google Scholar]
  2. Bai J., Zhu R. Y., Stedman K., Cousens C., Carlson J., Sharp J. M., DeMartini J. C. 1996; Unique long terminal repeat U3 sequences distinguish exogenous jaagsiekte sheep retroviruses associated with ovine pulmonary carcinoma from endogenous loci in the sheep genome. J Virol 70:3159–3168
    [Google Scholar]
  3. Blaise S., Mangeney M., Heidmann T. 2001; The envelope of Mason–Pfizer monkey virus has immunosuppressive properties. J Gen Virol 82:1597–1600
    [Google Scholar]
  4. Chamorro M., Parkin N., Varmus H. E. 1992; An RNA pseudoknot and an optimal heptameric shift site are required for highly efficient ribosomal frameshifting on a retroviral messenger RNA. Proc Natl Acad Sci U S A 89:713–717
    [Google Scholar]
  5. Chomczynsky P., Sacchi N. 1987; Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
    [Google Scholar]
  6. Cousens C., Minguijón E., García M., Ferrer L. M., Dalziel R. G., Palmarini M., De las Heras M., Sharp J. M. 1996; PCR-based detection and partial characterization of a retrovirus associated with contagious intranasal tumors of sheep and goats. J Virol 70:7580–7583
    [Google Scholar]
  7. Cousens C., Minguijón E., Dalziel R. G., Ortín A., García M., Park J., González L., Sharp J. M., De las Heras M. 1999; Complete sequence of enzootic nasal tumor virus, a retrovirus associated with transmissible intranasal tumors of sheep. J Virol 73:3986–3993
    [Google Scholar]
  8. De las Heras M., García de Jalón J. A., Sharp J. M. 1991a; Pathology of enzootic intranasal tumour in thirty-eight goats. Vet Pathol 28:474–481
    [Google Scholar]
  9. De las Heras M., Sharp J. M., García de Jalon J. A., Dewar P. 1991b; Enzootic nasal tumour of goats; demonstration of a type D-related retrovirus in nasal fluids and tumours. J Gen Virol 72:2533–2535
    [Google Scholar]
  10. De las Heras M., Sharp J. M., Ferrer L. M., García de Jalón J. A., Cebrian L. M. 1993; Evidence for a type D-like retrovirus in enzootic nasal tumor of sheep. Vet Rec 132:441
    [Google Scholar]
  11. De las Heras M., Ortín A., Cousens C., Minguijón E., Sharp J. M. 2003; Jaagsiekte sheep retrovirus and lung cancer. Enzootic nasal adenocarcinoma of sheep and goats. In Current Topics in Microbiology and Immunology , vol 275, chapter 2 Edited by Fan H. New York: Springer-Verlag;
    [Google Scholar]
  12. Denner J. 1998; Immunosuppression by retroviruses: implications for xenotransplantation. Ann N Y Acad Sci 862:75–86
    [Google Scholar]
  13. Genetics Computer Group; 1994 Program manual for the Wisconsin package version 8
    [Google Scholar]
  14. González L., García-Goti M., Cousens C., Dewar P., Cortabarría N., Extramiana A. B., Ortín A., De las Heras M., Sharp J. M. 2001; Jaagsiekte sheep retrovirus can be detected in the peripheral blood during the pre-clinical period of sheep pulmonary adenomatosis. J Gen Virol 82:1355–1358
    [Google Scholar]
  15. Hecht S. J., Carlson J. O., DeMartini J. C. 1994; Analysis of a type D retroviral capsid gene expressed in ovine pulmonary carcinoma and present in both affected and unaffected sheep genomes. Virology 202:480–484
    [Google Scholar]
  16. Herring A. J., Sharp J. M., Scott F. M. M., Angus K. W. 1983; Further evidence for a retrovirus as the aetiological agent of sheep pulmonary adenomatosis (jaagsiekte). Vet Microbiol 8:237–249
    [Google Scholar]
  17. Jacks T., Madhani H. D., Masiarz F. R., Varmus H. E. 1988; Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region. Cell 55:447–458
    [Google Scholar]
  18. Maeda N., Palmarini M., Murgia C., Fan H. 2001; Direct transformation of rodent fibroblasts by jaagsiekte sheep retrovirus DNA. Proc Natl Acad Sci U S A 98:4449–4454
    [Google Scholar]
  19. Ortín A., Minguijón E., Dewar P., Garcia M., Ferrer L. M., Palmarini M., González L., Sharp J. M., De las Heras M. 1998; Lack of a specific immune response against a recombinant capsid protein of jaagsiekte sheep retrovirus in sheep and goats naturally affected by enzootic nasal tumour or sheep pulmonary adenomatosis. Vet Immunol Immunopathol 61:229–237
    [Google Scholar]
  20. Palmarini M., Holland M. J., Cousens C., Dalziel R. G., Sharp J. M. 1996; Jaagsiekte retrovirus establishes a disseminated infection of the lymphoid tissues of sheep affected by pulmonary adenomatosis. J Gen Virol 77:2991–2998
    [Google Scholar]
  21. Palmarini M., Sharp J. M., De las Heras M., Fan H. 1999; Jaagsiekte sheep retrovirus is necessary and sufficient to induce a contagious lung cancer in sheep. J Virol 73:6964–6972
    [Google Scholar]
  22. Palmarini M., Hallwirth C., York D., Murgia C., De Oliveira T., Spencer T., Fan H. 2000; Molecular cloning and functional analysis of three type D endogenous retroviruses of sheep reveal a different cell tropism from that of the highly related exogenous jaagsiekte sheep retrovirus. J Virol 74:8065–8076
    [Google Scholar]
  23. Palmarini M., Maeda N., Murgia C., De-Fraja C., Hofracre A., Fan H. 2001; A phosphatidylinositol 3-kinase docking site in the cytoplasmic tail of jaagsiekte sheep retrovirus transmembrane protein is essential for envelope-induced transformation of NIH 3T3 cells. J Virol 75:11002–11009
    [Google Scholar]
  24. Palmarini M., Murgia C., Fan H. 2002; Spliced and prematurely polyadenylated jaagsiekte sheep retrovirus-specific RNAs from infected or transfected cells. Virology 294:180–188
    [Google Scholar]
  25. Rai S. K., Duh F. M., Vigdorovich V., Danilkovitch-Miagkova A., Lerman M. I., Miller D. 2001; Candidate tumor suppressor HYAL2 is a glycosylphosphatidylinositol (GPI)-anchored cell surface receptor for jaagsiekte sheep retrovirus, the envelope protein of which mediates oncogenic transformation. Proc Natl Acad Sci U S A 98:4443–4448
    [Google Scholar]
  26. Vitelozzi G., Mughetti L., Palmarini M., Mandara M. T., Mechelli L., Sharp J. M., Manocchio I. 1993; Enzootic intranasal tumour of goats in Italy. J Vet Med Ser B 40:459–468
    [Google Scholar]
  27. Wu Q., Chen M., Buchwald M., Phillips R. A. 1995; A simple rapid method for isolation of high quality genomic DNA from animal tissues. Nucleic Acids Res 23:5087–5088
    [Google Scholar]
  28. York D. F., Vigne R., Verwoerd D. W., Querat G. 1992; Nucleotide sequence of the jaagsiekte retrovirus, an exogenous and endogenous type D and B retrovirus of sheep and goats. J Virol 66:4930–4939
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19125-0
Loading
/content/journal/jgv/10.1099/vir.0.19125-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error