1887

Abstract

Attachment of, and cell–cell fusion induced by, human cytomegalovirus were studied in the presence of neutralizing monospecific antibodies against antigenic domains 1 (AD-1) or 2 (AD-2) of glycoprotein B (gB, gpUL55). Efficient inhibition of the virion-mediated fusion event was consistently observed for the human AD-2-specific antibody as determined by a reporter gene activation assay based on permissive astrocytoma cells. In contrast, antibodies directed against the major neutralizing gB epitope AD-1 reduced fusion only by 20–60 %. Virus attachment via heparan sulfate was unaffected by the antibodies under the conditions used. Virus receptor binding as examined by heparin treatment of adsorbed virus was significantly reduced only if the virus had been coated with the AD-2-specific antibody. Neutralization of virus infectivity by the AD-2-specific antibody thus seems most likely to result from interference with a receptor-binding event during initial virus–host cell interaction.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19017-0
2003-07-01
2019-09-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/7/vir841859.html?itemId=/content/journal/jgv/10.1099/vir.0.19017-0&mimeType=html&fmt=ahah

References

  1. Boyle, K. A. & Compton, T. ( 1998; ). Receptor-binding properties of a soluble form of human cytomegalovirus glycoprotein B. J Virol 72, 1826–1833.
    [Google Scholar]
  2. Britt, W. J., Vugler, L., Butfiloski, E. J. & Stephens, E. B. ( 1990; ). Cell surface expression of human cytomegalovirus (HCMV) gp55-116 (gB): use of HCMV-recombinant vaccinia virus-infected cells in analysis of the human neutralizing antibody response. J Virol 64, 1079–1085.
    [Google Scholar]
  3. Compton, T., Nowlin, D. M. & Cooper, N. R. ( 1993; ). Initiation of human cytomegalovirus infection requires initial interaction with cell surface heparin sulfate. Virology 193, 834–841.[CrossRef]
    [Google Scholar]
  4. Dimmock, N. J. ( 1993; ). Neutralization of animal viruses. Curr Top Microbiol Immunol 183, 1–149.
    [Google Scholar]
  5. Eggers, M., Bogner, E., Agricola, B., Kern, H. F. & Radsak, K. ( 1992; ). Inhibition of human cytomegalovirus maturation by brefeldin A. J Gen Virol 73, 2679–2692.[CrossRef]
    [Google Scholar]
  6. Gicklhorn, D., Eickmann, M. & Radsak, K. ( 1999; ). Chloramphenicol acetyltransferase expression as a sensor for fusion activity. Biotechniques 27, 902–904, 906, 908.
    [Google Scholar]
  7. Gonczol, E., deTaisne, C., Hirka, G., Berencsi, K., Lin, W. C., Paoletti, E. & Plotkin, S. ( 1991; ). High expression of human cytomegalovirus (HCMV)-gB protein in cells infected with a vaccinia-gB recombinant: the importance of the gB protein in HCMV immunity. Vaccine 9, 631–637.[CrossRef]
    [Google Scholar]
  8. Gorman, C. M., Moffat, L. F. & Howard, B. H. ( 1982; ). Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol 2, 1044–1051.
    [Google Scholar]
  9. Kari, B. & Gehrz, R. ( 1992; ). A human cytomegalovirus glycoprotein complex designated gC-II is a major heparin-binding component of the envelope. J Virol 66, 1761–1764.
    [Google Scholar]
  10. Kari, B. & Gehrz, R. ( 1993; ). Structure, composition and heparin binding properties of a human cytomegalovirus glycoprotein complex designated gC-II. J Gen Virol 74, 255–264.[CrossRef]
    [Google Scholar]
  11. Kniess, N., Mach, M., Fay, J. & Britt, W. J. ( 1991; ). Distribution of linear antigenic sites on glycoprotein gp55 of human cytomegalovirus. J Virol 65, 138–146.
    [Google Scholar]
  12. Marshall, G. S., Rabalais, G. P., Stout, G. G. & Waldeyer, S. L. ( 1992; ). Antibodies to recombinant-derived glycoprotein B after natural human cytomegalovirus infection correlate with neutralizing activity. J Infect Dis 165, 381–384.[CrossRef]
    [Google Scholar]
  13. Meyer, G. A. & Radsak, K. D. ( 2000; ). Identification of a novel signal sequence that targets transmembrane proteins to the nuclear envelope inner membrane. J Biol Chem 275, 3857–3866.[CrossRef]
    [Google Scholar]
  14. Meyer, H., Sundqvist, V. A., Pereira, L. & Mach, M. ( 1992; ). Glycoprotein gp116 of human cytomegalovirus contains epitopes for strain-common and strain-specific antibodies. J Gen Virol 73, 2375–2383.[CrossRef]
    [Google Scholar]
  15. Ohizumi, Y., Suzuki, H., Matsumoto, Y., Masuho, Y. & Numazaki, Y. ( 1992; ). Neutralizing mechanisms of two human monoclonal antibodies against human cytomegalovirus glycoprotein 130/55. J Gen Virol 73, 2705–2707.[CrossRef]
    [Google Scholar]
  16. Ohlin, M., Sundqvist, V. A., Gilljam, G., Ruden, U., Gombert, F. O., Wahren, B. & Borrebaeck, C. A. ( 1991; ). Characterization of human monoclonal antibodies directed against the pp65-kD matrix antigen of human cytomegalovirus. Clin Exp Immunol 84, 508–514.
    [Google Scholar]
  17. Ohlin, M., Sundqvist, V. A., Mach, M., Wahren, B. & Borrebaeck, C. A. ( 1993; ). Fine specificity of the human immune response to the major neutralization epitopes expressed on cytomegalovirus gp58/116 (gB), as determined with human monoclonal antibodies. J Virol 67, 703–710.
    [Google Scholar]
  18. Ohlin, M., Silvestri, M., Sundqvist, V. A. & Borrebaeck, C. A. ( 1997; ). Cytomegalovirus glycoprotein B-specific antibody analysis using electrochemiluminescence detection-based techniques. Clin Diagn Lab Immunol 4, 107–111.
    [Google Scholar]
  19. Radsak, K., Wiegandt, H., Unterdorfer, G., Wagner, C. & Kaiser, C. J. ( 1985; ). Sodium butyrate selectively inhibits host cell glycoprotein synthesis in human fibroblasts infected with cytomegalovirus. Biosci Rep 5, 589–599.[CrossRef]
    [Google Scholar]
  20. Schmolke, S., Drescher, P., Jahn, G. & Plachter, B. ( 1995; ). Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport. J Virol 69, 1071–1078.
    [Google Scholar]
  21. Silvestri, M. E. & Sundqvist, V. A. ( 2001; ). An investigation into the heparin-binding properties of a synthetic peptide deduced from the antigenic domain 2 of human cytomegalovirus glycoprotein B. Scand J Immunol 53, 282–289.[CrossRef]
    [Google Scholar]
  22. Silvestri, M., Sundqvist, V. A., Ruden, U. & Wahren, B. ( 1991; ). Characterization of a major antigenic region on gp55 of human cytomegalovirus. J Gen Virol 72, 3017–3023.[CrossRef]
    [Google Scholar]
  23. Wagner, B., Kropff, B., Kalbacher, H., Britt, W., Sundqvist, V. A., Ostberg, L. & Mach, M. ( 1992; ). A continuous sequence of more than 70 amino acids is essential for antibody binding to the dominant antigenic site of glycoprotein gp58 of human cytomegalovirus. J Virol 66, 5290–5297.
    [Google Scholar]
  24. Whitbeck, J. C., Muggeridge, M. I., Rux, A. H., Hou, W., Krummenacher, C., Lou, H., van Geelen, A., Eisenberg, R. J. & Cohen, G. H. ( 1999; ). The major neutralizing antigenic site on herpes simplex virus glycoprotein D overlaps a receptor-binding domain. J Virol 73, 9879–9890.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19017-0
Loading
/content/journal/jgv/10.1099/vir.0.19017-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error