1887

Abstract

Newcastle disease virus (NDV) was examined for its suitability as a vector for the expression and delivery of foreign genes for vaccination and gene therapy. A reporter gene encoding human secreted alkaline phosphatase (SEAP) was inserted as an additional transcription unit at four different positions in the NDV genome, between the NP and P, M and F, and HN and L genes and behind the L gene. Eight infectious recombinant NDV (rNDV) viruses, four in the non-virulent strain NDFL and four in the virulent derivative NDFLtag, were generated by reverse genetics. SEAP expression levels, replication kinetics and virus yield were examined. Replication kinetics of the rNDV viruses in primary chicken embryo fibroblasts showed that the insertion of an additional gene resulted in a delay in the onset of replication. This effect was most prominent when the gene was inserted between the NP and P genes. With the exception of the strain that carried the SEAP gene behind the L gene, all recombinant strains expressed high levels of SEAP, both in cell culture and in embryonated chicken eggs. In embryonated eggs, the rNDV viruses showed a 2·6- to 5·6-fold (NDFL) or 2·1- to 8·1-fold (NDFLtag) reduction in yield compared with the parent strains. These results show that foreign genes can be inserted at different positions in the NDV genome without severely affecting replication efficiency or virus yield.

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2003-04-01
2019-10-23
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References

  1. Abraham, G. & Banerjee, A. K. ( 1976; ). Sequential transcription of the genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A 73, 1504–1508.[CrossRef]
    [Google Scholar]
  2. Bailly, J. E., McAuliffe, J. M., Durbin, A. P., Elkins, W. R., Collins L., P. & Murphy, B. R. ( 2000; ). A recombinant parainfluenza virus type 3 (PIV3) in which the nucleocapsid N protein has been replaced by that of bovine PIV3 is attenuated in primates. J Virol 74, 3188–3195.[CrossRef]
    [Google Scholar]
  3. Ball, L. A. & White, C. N. ( 1976; ). Order of transcription of genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A 73, 442–446.[CrossRef]
    [Google Scholar]
  4. Baron, M. D., Foster-Cuevas, M., Baron, J. & Barrett, T. ( 1999; ). Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus. J Gen Virol 80, 2031–2039.
    [Google Scholar]
  5. Beard, C. W. & Hanson, R. P. ( 1984; ). Newcastle disease. In Disease of Poultry, pp. 452–470. Edited by M. S. Hofstad, H. J. Barnes, B. W. Calnek, W. M. Reid & H. W. Yoder. Ames, IA: Iowa State University Press.
  6. Berger, J., Hauber, J., Hauber, R., Geiger, T. & Cullen, B. R. ( 1988; ). Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells. Gene 66, 1–10.[CrossRef]
    [Google Scholar]
  7. Britton, P., Greek, P., Kottier, S., Mawditt, K. L., Penzes, Z., Cavanagh, D. & Skinner, M. ( 1996; ). Expression of bacteriophage T7 RNA polymerase in avian and mammalian cells by a recombinant fowlpox virus. J Gen Virol 77, 963–967.[CrossRef]
    [Google Scholar]
  8. Buchholz, U., Granzow, H., Schuldt, K., Whitehead, S. S., Murphy, B. R. & Collins, P. L. ( 2000; ). Chimeric bovine respiratory syncytial virus with glycoprotein gene substitutions from human respiratory syncytial virus (HRSV): effects on host range and evaluation as a live-attenuated HRSV vaccine. J Virol 74, 1187–1199.[CrossRef]
    [Google Scholar]
  9. Bukreyev, A., Camargo, E. & Collins, P. L. ( 1996; ). Recovery of infectious respiratory syncytial virus expressing an additional foreign gene. J Virol 70, 6634–6641.
    [Google Scholar]
  10. Calain, P. & Roux, L. ( 1993; ). The rule of six, a basic feature for efficient replication of Sendai virus defective interfering RNA. J Virol 67, 4822–4830.
    [Google Scholar]
  11. Chang, A. C. & Cohen. S. N. ( 1978; ). Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134, 1141–1156.
    [Google Scholar]
  12. Collins, P. L., McIntosh, K. & Chanock, R. M. ( 1996; ). Respiratory syncytial virus. In Fields Virology, 3rd edn, pp. 1313–1351. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  13. de Leeuw, O. & Peeters, B. ( 1999; ). Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae. J Gen Virol 80, 131–136.
    [Google Scholar]
  14. Durbin, A. P., Skiadopoulos, M. H., McAuliffe, J. M., Riggs, J. M., Surman, S. R., Collins, P. L. & Murphy, B. R. ( 2000; ). Human parainfluenza virus type 3 (PIV3) expressing the hemagglutinin protein of measles virus provides a potential method for immunization against measles virus and PIV3 in early infancy. J Virol 74, 6821–6831.[CrossRef]
    [Google Scholar]
  15. Egelman, E. H., Wu, S. S., Amrein, M., Portner, A. & Murti. G. ( 1989; ). The Sendai virus nucleocapsid exists in at least four different helical states. J Virol 63, 2233–2243.
    [Google Scholar]
  16. Emerson, S. U. ( 1982; ). Reconstitution studies detect a single polymerase entry site on the vesicular stomatitis virus genome. Cell 31, 635–642.[CrossRef]
    [Google Scholar]
  17. Goldhaft, T. M. ( 1980; ). Historical note on the origin of the La Sota strain of Newcastle disease virus. Avian Dis 24, 297–301.[CrossRef]
    [Google Scholar]
  18. He, B., Paterson, R. G., Ward, C. D. & Lamb, R. A. ( 1997; ). Recovery of infectious SV5 from cloned DNA and expression of a foreign gene. Virology 237, 249–260.[CrossRef]
    [Google Scholar]
  19. Hitchner, S. B. & Johnson, E. P. ( 1948; ). A virus of low virulence for immunizing fowls against Newcastle disease (avian pneumoencephalitis). Vet Med 43, 525–530.
    [Google Scholar]
  20. Huang, Z., Krishnamurthy, S., Panda, A. & Samal, S. K. ( 2001; ). High-level expression of a foreign gene from the most 3′-proximal locus of a recombinant Newcastle disease virus. J Gen Virol 82, 1729–1736.
    [Google Scholar]
  21. Iverson, L. E. & Rose, J. K. ( 1982; ). Sequential synthesis of 5′-proximal vesicular stomatitis virus mRNA sequences. J Virol 44, 356–365.
    [Google Scholar]
  22. Johnson, J. E., Schnell, M. J., Buonocore, L. & Rose, J. K. ( 1997; ). Specific targeting to CD4+ cells of recombinant vesicular stomatitis viruses encoding human immunodeficiency virus envelope proteins. J Virol 71, 5060–5068.
    [Google Scholar]
  23. Kolakofsky, D., Pelet, T., Garcin, D., Sausmann, S., Curran, J. & Roux, L. ( 1998; ). Paramyxovirus RNA synthesis and the requirement for hexamer genome length: the rule of six revisited. J Virol 72, 891–899.
    [Google Scholar]
  24. Krishnamurty, S. & Samal, S. K. ( 1998; ). Nucleotide sequence of the trailer, nucleocapsid protein gene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of the entire genome sequence. J Gen Virol 79, 2419–2424.
    [Google Scholar]
  25. Krishnamurthy, S., Huang, Z. & Samal, S. K. ( 2000; ). Recovery of a virulent strain of Newcastle disease virus from cloned cDNA: expression of a foreign gene results in growth retardation and attenuation. Virology 278, 168–182.[CrossRef]
    [Google Scholar]
  26. Lamb, R. A. & Kolakofsky, D. ( 1996; ). Paramyxoviridae: the viruses and their replication. In Fields Virology, 3rd edn, pp. 1177–1204. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  27. Lamb. R. A. & Krug, R. M. ( 1996; ). Orthomyxoviridae: the viruses and their replication. In Fields Virology, 3rd edn, pp. 1353–1395. Edited by B. N. Fields, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  28. Mebatsion, T., Schnell, M. J., Cox, J. H., Finke, S. & Conzelmann, K. ( 1996; ). Highly stable expression of a foreign gene from rabies virus vectors. Proc Natl Acad Sci U S A 93, 7310–7314.[CrossRef]
    [Google Scholar]
  29. Millar, N. S. & Emmerson, P. T. ( 1998; ). Molecular cloning and nucleotide sequencing of Newcastle disease virus. In Newcastle Disease, pp. 79–97. Edited by D. J. Alexander. Boston, MA: Kluwer Academic.
  30. Murphy, S. K. & Parks, G. D. ( 1997; ). Genome nucleotide lengths that are divisible by six are not essential but enhance replication of defective interfering RNAs of the paramyxovirus simian virus 5. Virology 232, 145–147.[CrossRef]
    [Google Scholar]
  31. Nakaya, T., Cros, J., Park, M.-S., Nakaya, Y., Zheng, H., Sagrera, A., Villar, E., Garcia-Sastre, A. & Palese, P. ( 2001; ). Recombinant Newcastle disease virus as a vaccine vector. J Virol 75, 11868–11873.[CrossRef]
    [Google Scholar]
  32. Pattnaik, A. K. & Wertz, G. W. ( 1990; ). Replication and amplification of defective interfering particle RNAs of vesicular stomatitis virus in cells expressing viral proteins from vectors containing cloned cDNA. J Virol 64, 2948–2957.
    [Google Scholar]
  33. Peeters, B. P. H., de Leeuw, O. S., Koch, G. & Gielkens, A. L. J. ( 1999; ). Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. J Virol 73, 5001–5009.
    [Google Scholar]
  34. Peeters, B. P. H., Gruijthuijsen, Y. K., de Leeuw, O. S. & Gielkens, A. L. J. ( 2000; ). Genome replication of Newcastle disease virus: involvement of the rule-of-six. Arch Virol 145, 1829–1845.[CrossRef]
    [Google Scholar]
  35. Phillips, R. J., Samson, A. C. R. & Emmerson, P. T. ( 1998; ). Nucleotide sequence of the 5′terminus of Newcastle disease virus and assembly of the complete genomic sequence: agreement with the “rule of six”. Arch Virol 143, 1993–2002.[CrossRef]
    [Google Scholar]
  36. Roberts, A., Kretzschmar, E., Perkins, A. S., Forman, J., Price, R., Buonocore, L., Kawaoka, Y. & Rose, J. K. ( 1998; ). Vaccination with a recombinant vesicular stomatitis virus expressing an influenza virus hemagglutinin provides complete protection from influenza virus challenge. J Virol 72, 4704–4711.
    [Google Scholar]
  37. Römer-Oberdörfer, A., Mundt, E., Mebatsion, T., Buchholz, U. & Mettenleiter, T. C. ( 1999; ). Generation of recombinant lentogenic Newcastle disease virus from cDNA. J Gen Virol 80, 2987–2995.
    [Google Scholar]
  38. Sakai, Y., Kiyotami, K., Fukumara, M. & 7 other authors ( 1999; ). Accommodation of foreign genes into the Sendai virus genome: size of inserted genes and viral replication. FEBS Lett 456, 211–226.[CrossRef]
    [Google Scholar]
  39. Singh, M. & Billeter, M. A. ( 1999; ). A recombinant measles virus expressing biologically active human interleukin-12. J Gen Virol 80, 101–106.
    [Google Scholar]
  40. Wensvoort, G., Terpstra, C., Boonstra, J., Bloemraad, M. & Van Zaane, D. ( 1986; ). Production of monoclonal antibodies against swine fever virus and their use in laboratory diagnosis. Vet Microbiol 12, 101–108.[CrossRef]
    [Google Scholar]
  41. Wertz, G. W., Perepelitis, V. P. & Ball, L. A.. ( 1998; ). Gene rearrangement and attenuated expression and lethality of a nonsegmented negative strand RNA virus. Proc Natl Acad Sci U S A 92, 8388–8392.
    [Google Scholar]
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