1887

Abstract

Five fish cell lines were tested for their ability to be transduced by Ac-CA, a recombinant baculovirus that is capable of expressing a -galactosidase reporter gene from the CAG promoter (consisting of a cytomegalovirus enhancer element, a chicken actin promoter and rabbit -globin termination sequences). TO ( ovary), EPC (carp), CHH-1 (Chum salmon heart fibroblast) and CHSE-214 (chinook salmon embryo) cells were transducible, as demonstrated by an -galactosidase assay, whereas RTG-2 (rainbow trout gonad) cells were not. The EPC cell line was used for more detailed studies on baculovirus transduction. The transduction frequency was found to be higher at 28 °C than at 21 °C. Addition of the histone deacetylase inhibitor sodium butyrate increased the number of blue cells detected 5- to 7-fold. The m.o.i. was positively correlated with transduction frequency, although the relationship did not appear to be strictly linear, as has been observed with mammalian cells. The temperature at which baculoviruses were adsorbed to EPC cells did not affect levels of -galactosidase expression. We also examined expression levels of -galactosidase in EPC cells after infection with a baculovirus construct that overexpresses the vesicular stomatitis virus G protein and displays it on the virion surface. Expression levels with this virus were approximately 15-fold higher than were observed with Ac-CA.

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2003-05-01
2019-10-21
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References

  1. Anderson, E. D., Mourich, D. V., Fahrenkrug, S. C., LaPatra, S., Shepherd, J. & Leong, J. A. ( 1996; ). Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus. Mol Mar Biol Biotechnol 5, 114–122.
    [Google Scholar]
  2. Barsoum, J., Brown, R., McKee, M. & Boyce, F. M. ( 1997; ). Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein. Hum Gene Ther 8, 2011–2018.[CrossRef]
    [Google Scholar]
  3. Blissard, G. W. & Wenz, J. R. ( 1992; ). Baculovirus gp64 envelope glycoprotein is sufficient to mediate pH-dependent membrane fusion. J Virol 66, 6829–6835.
    [Google Scholar]
  4. Chen, S. N., Chi, S. C., UIeno, Y. & Kou, G. H. ( 1983; ). A cell line derived from tilapia ovary. Fish Pathol 18, 13–18.[CrossRef]
    [Google Scholar]
  5. Condreay, J. P., Witherspoon, S. M., Clay, W. C. & Kost, T. A. ( 1999; ). Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci U S A 96, 127–132.[CrossRef]
    [Google Scholar]
  6. Corbeil, S., Kurath, G. & LaPatra, S. E. ( 2000; ). Fish DNA vaccine against infectious hematopoietic necrosis virus: efficacy of various routes of immunisation. Fish Shellfish Immunol 10, 711–723.[CrossRef]
    [Google Scholar]
  7. Duisit, G., Saleun, S., Douthe, S., Barsoum, J., Chadeuf, G. & Moullier, P. ( 1999; ). Baculovirus vector requires electrostatic interactions including heparan sulfate for efficient gene transfer in mammalian cells. J Gene Med 1, 93–102.[CrossRef]
    [Google Scholar]
  8. Fernandez-Alonso, M., Rocha, A. & Coll, J. M. ( 2001; ). DNA vaccination by immersion and ultrasound to trout viral haemorrhagic septicaemia virus. Vaccine 19, 3067–3075.[CrossRef]
    [Google Scholar]
  9. Fijan, N., Sulimanovic, D., Bearzottti, M., Muzinic, D., Zwillenberg, L. O., Chilmonczyk, S., Vautherot, J. F. & de Kinkelin, P. ( 1983; ). Some properties of the epithelioma papulosum cyprini (EPC) cell line from carp Cyprinus carpio. Ann Virol 134E, 207–220.
    [Google Scholar]
  10. Hayakawa, T., Rohrmann, G. F. & Hashimoto, Y. ( 2000; ). Patterns of genome organization and content in lepidopteran baculoviruses. Virology 278, 1–12.[CrossRef]
    [Google Scholar]
  11. Hefferon, K. L., Oomens, A. G., Monsma, S. A., Finnerty, C. M. & Blissard, G. W. ( 1999; ). Host cell receptor binding by baculovirus GP64 and kinetics of virion entry. Virology 258, 455–468.[CrossRef]
    [Google Scholar]
  12. Kost, T. A. & Condreay, J. P. ( 2002; ). Recombinant baculoviruses as mammalian cell gene-delivery vectors. Trends Biotechnol 20, 173–180.[CrossRef]
    [Google Scholar]
  13. Kruh, J. ( 1982; ). Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol Cell Biochem. 42, 65–82.
    [Google Scholar]
  14. Lannan, C. N., Winton, J. R. & Fryer, J. L. ( 1984; ). Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20, 671–676.[CrossRef]
    [Google Scholar]
  15. Martignoni, M. E. & Iwai, P. J. ( 1986; ). A Catalog of Viral Diseases of Insects, Mites, and Ticks. Washington, DC: USGPO/USDA Forest Service PNW-195.
  16. Niwa, H., Yamamura, K. & Miyazaki, J. ( 1991; ). Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193–199.[CrossRef]
    [Google Scholar]
  17. O'Reilly, D. R., Miller, L. K. & Luckow, V. A. ( 1992; ). Baculovirus Expression Vectors. New York: W. H. Freeman.
  18. Shoji, I., Aizaki, H., Tani, H., Ishii, K., Chiba, T., Saito, I., Miyamura, T. & Matsuura, Y. ( 1997; ). Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J Gen Virol 78, 2657–2664.
    [Google Scholar]
  19. Tani, H., Nishijima, M., Ushijima, H., Miyamura, T. & Matsuura, Y. ( 2001; ). Characterization of cell-surface determinants important for baculovirus infection. Virology 279, 343–353.[CrossRef]
    [Google Scholar]
  20. Wilson, M. E. & Miller, L. K. ( 1986; ). Changes in the nucleoprotein complexes of a baculovirus DNA during infection. Virology 151, 315–328.[CrossRef]
    [Google Scholar]
  21. Wolf, K. & Quimby, M. C. ( 1962; ). Established eurythermic line of fish cells in vitro. Science 135, 1065–1066.[CrossRef]
    [Google Scholar]
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