1887

Journal of General Virology

Volume 84, Issue 7

Construction and infection of a new simian/human immunodeficiency virus chimera containing the reverse transcriptase gene and the 3′ half of the genomic region of human immunodeficiency virus type 1 Free

Abstract

A new simian/human immunodeficiency virus (SHIV) chimera with the reverse transcriptase (RT)-encoding region of , in addition to the 3′ region encoding , , , , and of HIV-1, on an SIV (SIV from a macaque monkey) background was constructed. This new SHIV chimera, named SHIVrt/3rn, could replicate in monkey peripheral blood mononuclear cells (PBMCs) as well as in the human and monkey CD4 T-cell lines M8166 and HSC-F. Since SHIVrt/3rn contains the RT gene of HIV-1, replication of the virus in M8166 cells was inhibited by an HIV-1-specific non-nucleoside RT inhibitor, MKC-442, with a sensitivity similar to that of HIV-1. To investigate the replication competence of SHIVrt/3rn , two rhesus monkeys were inoculated intravenously with the virus. At 2 to 4 weeks post-inoculation (p.i.), plasma viral RNA loads of both monkeys showed a peak value of more than 10 copies ml. Infectious virus was isolated from the PBMCs of one monkey at 2 and 3 weeks p.i. and from the other at 4 weeks p.i. Moreover, proviral DNA was detected constantly throughout the observation period, starting from 3 weeks p.i. An antibody response, detected first at 3 weeks p.i., was maintained at high titres. These results indicate that SHIVrt/3rn can infect and replicate . SHIVrt/3rn, having part of HIV-1 in addition to the 3′ part of the HIV-1 genome is genetically more close to HIV-1 than any of the other monkey-infecting SHIVs reported previously.

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2003-07-01
2021-10-21
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References

  1. Adachi A., Gendelman H. E., Koenig S., Folks T., Willey R., Rabson A., Martin M. A. 1986; Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone. J Virol 59:284–291
     [Google Scholar]
  2. Akari H., Mori K., Terao K., Otani I., Fukasawa M., Mukai R., Yoshikawa Y. 1996; In vitro immortalization of Old World monkey T lymphocytes with herpesvirus saimiri: susceptibility to infection with simian immunodeficiency viruses. Virology 218:382–388
     [Google Scholar]
  3. Baba M., Shigeta S., Yuasa S. 7 other authors 1994; Preclinical evaluation of MKC-442, a highly potent and specific inhibitor of human immunodeficiency virus type 1 in vitro . Antimicrob Agents Chemother 38:688–692
     [Google Scholar]
  4. Bogers W. M., Dubbes R., ten Haaft P. 12 other authors 1997; Comparison of in vitro and in vivo infectivity of different clade B HIV-1 envelope chimeric simian/human immunodeficiency viruses in Macaca mulatta . Virology 236:110–117
     [Google Scholar]
  5. Clapham P. R., Weiss R. A., Dalgleish A. G., Exley M., Whitby D., Hogg N. 1987; Human immunodeficiency virus infection of monocytic and T-lymphocytic cells: receptor modulation and differentiation induced by phorbol ester. Virology 158:44–51
     [Google Scholar]
  6. Daniel M. D., Kirchhoff F., Czajak S. C., Sehgal P. K., Desrosiers R. C. 1992; Protective effects of a live attenuated SIV vaccine with a deletion in the nef gene. Science 258:1938–1941
     [Google Scholar]
  7. Fultz P. N., McClure H. M., Daugharty H., Brodie A., McGrath C. R., Swenson B., Francis D. P. 1986; Vaginal transmission of human immunodeficiency virus (HIV) to a chimpanzee. J Infect Dis 154:896–900
     [Google Scholar]
  8. Gajdusek D. C., Amyx H. L., Gibbs C. J. Jr & 7 other authors; 1985; Infection of chimpanzees by human T-lymphotropic retroviruses in brain and other tissues from AIDS patients. Lancet i55–56
     [Google Scholar]
  9. Harouse J. M., Gettie A., Tan R. C., Blanchard J., Cheng-Mayer C. 1999; Distinct pathogenic sequela in rhesus macaques infected with CCR5 or CXCR4 utilizing SHIVs. Science 284:816–819
     [Google Scholar]
  10. Hayami M., Igarashi T., Kuwata T., Ui M., Haga T., Ami Y., Shinohara K., Honda M. 1999; Gene-mutated HIV-1/SIV chimeric viruses as AIDS live attenuated vaccines for potential human use. Leukemia 13 (Suppl. 1):S42–S47
     [Google Scholar]
  11. Igarashi T., Shibata R., Hasebe F. 7 other authors 1994; Persistent infection with SIVmac chimeric virus having tat ,rev , vpu , env and nef of HIV type 1 in macaque monkeys. AIDS Res Hum Retroviruses 10:1021–1029
     [Google Scholar]
  12. Igarashi T., Kuwata T., Takehisa J. 7 other authors 1996; Genomic and biological alteration of a human immunodeficiency virus type 1 (HIV-1)-simian immunodeficiency virus strain mac chimera, with HIV-1 Env, recovered from a long-term carrier monkey. J Gen Virol 77:1649–1658
     [Google Scholar]
  13. Joag S. V., Li Z., Foresman L., Stephens E. B., Zhao L. J., Adany I., Pinson D. M., McClure H. M., Narayan O. 1996; Chimeric simian/human immunodeficiency virus that causes progressive loss of CD4+ T cells and AIDS in pig-tailed macaques. J Virol 70:3189–3197
     [Google Scholar]
  14. Joag S. V., Li Z., Foresman L. 10 other authors 1997; Characterization of the pathogenic KU-SHIV model of acquired immunodeficiency syndrome in macaques. AIDS Res Hum Retroviruses 13:635–645
     [Google Scholar]
  15. Kestler H. W. D. III, Ringler D. J., Mori K., Panicali D. L., Sehgal P. K., Daniel M. D., Desrosiers R. C. 1991; Importance of the nef gene for maintenance of high virus loads and for development of AIDS. Cell 65:651–662
     [Google Scholar]
  16. Kozyrev I. L., Miura T., Takemura T., Kuwata T., Ui M., Ibuki K., Iida T., Hayami M. 2002; Co-expression of interleukin-5 influences replication of simian/human immunodeficiency viruses in vivo . J Gen Virol 83:1183–1188
     [Google Scholar]
  17. Kuwata T., Igarashi T., Ido E., Jin M., Mizuno A., Chen J., Hayami M. 1995; Construction of human immunodeficiency virus 1/simian immunodeficiency virus strain mac chimeric viruses having vpr and/or nef of different parental origins and their in vitro and in vivo replication. J Gen Virol 76:2181–2191
     [Google Scholar]
  18. Letvin N. L., Daniel M. D., Sehgal P. K. 7 other authors 1985; Induction of AIDS-like disease in macaque monkeys with T-cell tropic retrovirus STLV-III. Science 230:71–73
     [Google Scholar]
  19. Li J., Lord C. I., Haseltine W., Letvin N. L., Sodroski J. 1992; Infection of cynomolgus monkeys with a chimeric HIV-1/SIVmac virus that expresses the HIV-1 envelope glycoproteins. J Acquir Immune Defic Syndr 5:639–646
     [Google Scholar]
  20. Naidu Y. M., Kestler H. W. D. III, Li Y. 8 other authors 1988; Characterization of infectious molecular clones of simian immunodeficiency virus (SIVmac) and human immunodeficiency virus type 2: persistent infection of rhesus monkeys with molecularly cloned SIVmac . J Virol 62:4691–4696
     [Google Scholar]
  21. Novembre F. J., Saucier M., Anderson D. C. 7 other authors 1997; Development of AIDS in a chimpanzee infected with human immunodeficiency virus type 1. J Virol 71:4086–4091
     [Google Scholar]
  22. Pauwels R., Balzarini J., Baba M., Snoeck R., Schols D., Herdewijn P., Desmyter J., De Clercq E. 1988; Rapid and automated tetrazolium-based colorimetric assay for the detection of anti-HIV compounds. J Virol Methods 20:309–321
     [Google Scholar]
  23. Reimann K. A., Li J. T., Veazey R., Halloran M., Park I. W., Karlsson G. B., Sodroski J., Letvin N. L. 1996; A chimeric simian/human immunodeficiency virus expressing a primary patient human immunodeficiency virus type 1 isolate env causes an AIDS-like disease after in vivo passage in rhesus monkeys. J Virol 70:6922–6928
     [Google Scholar]
  24. Shibata R., Kawamura M., Sakai H., Hayami M., Ishimoto A., Adachi A. 1991; Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells. J Virol 65:3514–3520
     [Google Scholar]
  25. Überla K., Stahl-Hennig C., Bottiger D. 7 other authors 1995; Animal model for the therapy of acquired immunodeficiency syndrome with reverse transcriptase inhibitors. Proc Natl Acad Sci U S A 92:8210–8214
     [Google Scholar]
  26. Ui M., Kuwata T., Igarashi T. 9 other authors 1999; Protection of macaques against a SHIV with a homologous HIV-1 Env and a pathogenic SHIV-89.6P with a heterologous Env by vaccination with multiple gene-deleted SHIVs. Virology 265:252–263
     [Google Scholar]
  27. Veazey R. S., DeMaria M., Chalifoux L. V. 7 other authors 1998; Gastrointestinal tract as a major site of CD4+ T cell depletion and viral replication in SIV infection. Science 280:427–431
     [Google Scholar]
  28. Willey R. L., Smith D. H., Lasky L. A., Theodore T. S., Earl P. L., Moss B., Capon D. J., Martin M. A. 1988; In vitro mutagenesis identifies a region within the envelope gene of the human immunodeficiency virus that is critical for infectivity. J Virol 62:139–147
     [Google Scholar]
  29. Yuasa S., Sadakata Y., Takashima H., Sekiya K., Inouye N., Ubasawa M., Baba M. 1993; Selective and synergistic inhibition of human immunodeficiency virus type 1 reverse transcriptase by a non-nucleoside inhibitor, MKC-442. Mol Pharmacol 44:895–900
     [Google Scholar]
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Construction and in vivo infection of a new simian/human immunodeficiency virus chimera containing the reverse transcriptase gene and the 3′ half of the genomic region of human immunodeficiency virus type 1
J. Gen. Virol. 84, 1663 (2003); https://doi.org/10.1099/vir.0.18843-0
/content/journal/jgv/10.1099/vir.0.18843-0
/content/journal/jgv/10.1099/vir.0.18843-0
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