1887

Abstract

A candidate gene of the bovine leukaemia virus (BLV) receptor (BLVR) was cloned previously and predicted to encode a transmembrane protein. Subsequent cloning of related genes from other organisms indicated that the candidate gene is related, but unique, to a gene family of the subunit of the adaptor protein (AP) complex 3, AP-3. Therefore, bovine cDNAs (boAP3) that are highly homologous to the candidate gene were cloned and sequenced. The nucleotide sequences suggested that the boAP3 cDNA encodes the subunit of boAP3 without transmembrane domains. Part of the AP3 cDNA isolated from the lymph node, spleen and MDBK cells, from which the BLVR candidate cDNA was derived, has almost the same nucleotide sequences as the boAP3 cDNA. A boAP3 protein tagged with green fluorescent protein was localized in the cytoplasm and incorporated into AP-3 in bovine cells. Unlike the previous report about the candidate gene, the boAP3 gene introduced into murine NIH 3T3 cells did not increase the susceptibility of the cells to BLV infection. Many small insertions and deletions of nucleotides could generate the predicted transmembrane and cytoplasmic regions of the BLVR protein from the prototypic boAP3 gene.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.18763-0
2003-05-01
2019-09-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/5/vir841309.html?itemId=/content/journal/jgv/10.1099/vir.0.18763-0&mimeType=html&fmt=ahah

References

  1. Ban, J., Portetelle, D., Altaner, C., Horion, B., Milan, D., Krchnak, V., Burny, A. & Kettmann, R. ( 1993; ). Isolation and characterization of a 2·3-kilobase-pair cDNA fragment encoding the binding domain of the bovine leukemia virus cell receptor. J Virol 67, 1050–1057.
    [Google Scholar]
  2. Ban, J., Truong, A. T., Horion, B., Altaner, C., Burny, A., Portetelle, D. & Kettmann, R. ( 1994; ). Isolation of the missing 5′-end of the encoding region of the bovine leukemia virus cell receptor gene. Arch Virol 138, 379–383.[CrossRef]
    [Google Scholar]
  3. Boehm, M. & Bonifacino, J. S. ( 2001; ). Adaptins: the final recount. Mol Biol Cell 12, 2907–2920.[CrossRef]
    [Google Scholar]
  4. Boehm, M. & Bonifacino, J. S. ( 2002; ). Genetic analysis of adaptin function from yeast to mammals. Gene 286, 175–186.[CrossRef]
    [Google Scholar]
  5. Dell'Angelica, E. C., Ohno, H., Ooi, C. E., Rabinovich, E., Roche, K. W. & Bonifacino, J. S. ( 1997; ). AP-3: an adaptor-like protein complex with ubiquitous expression. EMBO J 16, 917–928.[CrossRef]
    [Google Scholar]
  6. Derse, D. & Martarano, L. ( 1990; ). Construction of a recombinant bovine leukemia virus vector for analysis of virus infectivity. J Virol 64, 401–405.
    [Google Scholar]
  7. Fischinger, P. J., Blevins, C. S. & Nomura, S. ( 1974; ). Simple, quantitative assay for both xenotropic murine leukemia and ecotropic feline leukemia viruses. J Virol 14, 177–179.
    [Google Scholar]
  8. Graves, D. C. & Ferrer, J. F. ( 1976; ). In vitro transmission and propagation of the bovine leukemia virus in monolayer cell cultures. Cancer Res 36, 4152–4159.
    [Google Scholar]
  9. Kantheti, P., Qiao, X., Diaz, M. E. & 8 other authors ( 1998; ). Mutation in AP-3 δ in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles. Neuron 21, 111–122.[CrossRef]
    [Google Scholar]
  10. Kubota, T., Hikono, H., Sasaki, E. & Sakurai, M. ( 1994; ). Sequence of a bovine c-kit proto-oncogene cDNA. Gene 141, 305–306.[CrossRef]
    [Google Scholar]
  11. McClurkin, A. W., Pirtle, E. C., Coria, M. F. & Smith, R. L. ( 1974; ). Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Arch Gesamte Virusforsch 45, 285–289.[CrossRef]
    [Google Scholar]
  12. Multhoff, G. & Hightower, L. E. ( 1996; ). Cell surface expression of heat shock proteins and the immune response. Cell Stress Chaperones 1, 167–176.[CrossRef]
    [Google Scholar]
  13. Ooi, C. E., Moreira, J. E., Dell'Angelica, E. C., Poy, G., Wassarman, D. A. & Bonifacino, J. S. ( 1997; ). Altered expression of a novel adaptin leads to defective pigment granule biogenesis in the Drosophila eye color mutant garnet. EMBO J 16, 4508–4518.[CrossRef]
    [Google Scholar]
  14. Robinson, M. S. & Bonifacino, J. S. ( 2001; ). Adaptor-related proteins. Curr Opin Cell Biol 13, 444–453.[CrossRef]
    [Google Scholar]
  15. Schledzewski, K., Brinkmann, H. & Mendel, R. R. ( 1999; ). Phylogenetic analysis of components of the eukaryotic vesicle transport system reveals a common origin of adaptor protein complexes 1, 2, and 3 and the F subcomplex of the coatomer COPI. J Mol Evol 48, 770–778.[CrossRef]
    [Google Scholar]
  16. Short, J. M., Fernadez, J. M., Sorge, J. A. & Huse, W. D. ( 1988; ). λ ZAP: a bacteriophage λ expression vector with in vivo excision properties. Nucleic Acids Res 16, 7583–7600.[CrossRef]
    [Google Scholar]
  17. Simpson, F., Bright, N. A., West, M. A., Newman, L. S., Darnell, R. B. & Robinson, M. S. ( 1996; ). A novel adaptor-related protein complex. J Cell Biol 133, 749–760.[CrossRef]
    [Google Scholar]
  18. Simpson, F., Peden, A. A., Christopoulou, L. & Robinson, M. S. ( 1997; ). Characterization of the adaptor-related protein complex, AP-3. J Cell Biol 137, 835–845.[CrossRef]
    [Google Scholar]
  19. Sommerfelt, M. A. ( 1999; ). Retrovirus receptors. J Gen Virol 80, 3049–3064.
    [Google Scholar]
  20. Suzuki, T. & Ikeda, H. ( 1998; ). The mouse homolog of the bovine leukemia virus receptor is closely related to the delta subunit of adaptor-related protein complex AP-3, not associated with the cell surface. J Virol 72, 593–599.
    [Google Scholar]
  21. Toye, P. G., MacHugh, N. D., Bensaid, A. M., Alberti, S., Teale, A. J. & Morrison, W. I. ( 1990; ). Transfection into mouse L cells of genes encoding two serologically and functionally distinct bovine class I MHC molecules from a MHC-homozygous animal: evidence for a second class I locus in cattle. Immunology 70, 20–26.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.18763-0
Loading
/content/journal/jgv/10.1099/vir.0.18763-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error