1887

Abstract

An unusual rotavirus strain, Ryukyu-1120, with G5P[6] genotypes (RVA/Human-wt/JPN/Ryukyu-1120/2011/G5P[6]) was identified in a stool specimen from a hospitalized child aged 4 years who showed diarrhoea and encephalopathy. In this study, we sequenced and characterized the complete genome of strain Ryukyu-1120. On whole genomic analysis, this strain was found to have a unique genotype constellation: G5-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The VP6 and NSP1 genotypes I5 and A8 are those commonly found in porcine strains. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain Ryukyu-1120 appeared to be of porcine origin. Thus, strain Ryukyu-1120 was found to have a porcine rotavirus genetic backbone and is likely to be of porcine origin. To our knowledge, this is the first report of whole-genome-based characterization of the emerging G5P[6] strains in Asian countries. Our observations will provide important insights into the origin of G5P[6] strains and the dynamic interactions between human and porcine rotavirus strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.051011-0
2013-07-01
2020-01-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/94/7/1568.html?itemId=/content/journal/jgv/10.1099/vir.0.051011-0&mimeType=html&fmt=ahah

References

  1. Ahmed K. , Anh D. D. , Nakagomi O. . ( 2007; ). Rotavirus G5P[6] in child with diarrhea, Vietnam. . Emerg Infect Dis 13:, 1232–1235. [CrossRef] [PubMed]
    [Google Scholar]
  2. Beards G. , Graham C. . ( 1995; ). Temporal distribution of rotavirus G-serotypes in the West Midlands region of the United Kingdom, 1983-1994. . J Diarrhoeal Dis Res 13:, 235–237.[PubMed]
    [Google Scholar]
  3. Bok K. , Castagnaro N. , Borsa A. , Nates S. , Espul C. , Fay O. , Fabri A. , Grinstein S. , Miceli I. . & other authors ( 2001; ). Surveillance for rotavirus in Argentina. . J Med Virol 65:, 190–198. [CrossRef] [PubMed]
    [Google Scholar]
  4. Coluchi N. , Munford V. , Manzur J. , Vazquez C. , Escobar M. , Weber E. , Mármol P. , Rácz M. L. . ( 2002; ). Detection, subgroup specificity, and genotype diversity of rotavirus strains in children with acute diarrhea in Paraguay. . J Clin Microbiol 40:, 1709–1714. [CrossRef] [PubMed]
    [Google Scholar]
  5. Duan Z.-J. , Li D.-D. , Zhang Q. , Liu N. , Huang C.-P. , Jiang X. , Jiang B. , Glass R. , Steele D. . & other authors ( 2007; ). Novel human rotavirus of genotype G5P[6] identified in a stool specimen from a Chinese girl with diarrhea. . J Clin Microbiol 45:, 1614–1617. [CrossRef] [PubMed]
    [Google Scholar]
  6. Esona M. D. , Armah G. E. , Geyer A. , Steele A. D. . ( 2004; ). Detection of an unusual human rotavirus strain with G5P[8] specificity in a Cameroonian child with diarrhea. . J Clin Microbiol 42:, 441–444. [CrossRef] [PubMed]
    [Google Scholar]
  7. Esona M. D. , Geyer A. , Banyai K. , Page N. , Aminu M. , Armah G. E. , Hull J. , Steele D. A. , Glass R. I. , Gentsch J. R. . ( 2009; ). Novel human rotavirus genotype G5P[7] from child with diarrhea, Cameroon. . Emerg Infect Dis 15:, 83–86. [CrossRef] [PubMed]
    [Google Scholar]
  8. Estes M. K. , Kapikian A. Z. . ( 2007; ). Rotaviruses. . In Fields Virology, , 5th edn., pp. 1917–1974. Edited by Knipe D. M. , Howley P. M. . . Philadelphia:: Lippincott Williams & Wilkins;.
    [Google Scholar]
  9. Ghosh S. , Kobayashi N. . ( 2011; ). Whole-genomic analysis of rotavirus strains: current status and future prospects. . Future Microbiol 6:, 1049–1065. [CrossRef] [PubMed]
    [Google Scholar]
  10. Ghosh S. , Kobayashi N. , Nagashima S. , Chawla-Sarkar M. , Krishnan T. , Ganesh B. , Naik T. N. . ( 2010; ). Full genomic analysis and possible origin of a porcine G12 rotavirus strain RU172. . Virus Genes 40:, 382–388. [CrossRef] [PubMed]
    [Google Scholar]
  11. Ghosh S. , Urushibara N. , Taniguchi K. , Kobayashi N. . ( 2012; ). Whole genomic analysis reveals the porcine origin of human G9P[19] rotavirus strains Mc323 and Mc345. . Infect Genet Evol 12:, 471–477. [CrossRef] [PubMed]
    [Google Scholar]
  12. Gouvea V. , de Castro L. , Timenetsky M. C. , Greenberg H. , Santos N. . ( 1994; ). Rotavirus serotype G5 associated with diarrhea in Brazilian children. . J Clin Microbiol 32:, 1408–1409.[PubMed]
    [Google Scholar]
  13. Heiman E. M. , McDonald S. M. , Barro M. , Taraporewala Z. F. , Bar-Magen T. , Patton J. T. . ( 2008; ). Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions. . J Virol 82:, 11106–11116. [CrossRef] [PubMed]
    [Google Scholar]
  14. Hwang K.-P. , Wu F.-T. , Bányai K. , Wu H.-S. , Yang D. C.-F. , Huang Y.-C. , Lin J.-S. , Hsiung C. A. , Huang J. C. . & other authors ( 2012; ). Identification of porcine rotavirus-like genotype P[6] strains in Taiwanese children. . J Med Microbiol 61:, 990–997. [CrossRef] [PubMed]
    [Google Scholar]
  15. Li D.-D. , Duan Z.-J. , Zhang Q. , Liu N. , Xie Z.-P. , Jiang B. , Steele D. , Jiang X. , Wang Z.-S. , Fang Z.-Y. . ( 2008; ). Molecular characterization of unusual human G5P[6] rotaviruses identified in China. . J Clin Virol 42:, 141–148. [CrossRef] [PubMed]
    [Google Scholar]
  16. Maes P. , Matthijnssens J. , Rahman M. , Van Ranst M. . ( 2009; ). RotaC: a web-based tool for the complete genome classification of group A rotaviruses. . BMC Microbiol 9:, 238. [CrossRef] [PubMed]
    [Google Scholar]
  17. Martella V. , Bányai K. , Ciarlet M. , Iturriza-Gómara M. , Lorusso E. , De Grazia S. , Arista S. , Decaro N. , Elia G. . & other authors ( 2006; ). Relationships among porcine and human P[6] rotaviruses: evidence that the different human P[6] lineages have originated from multiple interspecies transmission events. . Virology 344:, 509–519. [CrossRef] [PubMed]
    [Google Scholar]
  18. Martella V. , Bányai K. , Matthijnssens J. , Buonavoglia C. , Ciarlet M. . ( 2010; ). Zoonotic aspects of rotaviruses. . Vet Microbiol 140:, 246–255. [CrossRef] [PubMed]
    [Google Scholar]
  19. Matthijnssens J. , Ciarlet M. , Heiman E. , Arijs I. , Delbeke T. , McDonald S. M. , Palombo E. A. , Iturriza-Gómara M. , Maes P. . & other authors ( 2008; ). Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains. . J Virol 82:, 3204–3219. [CrossRef] [PubMed]
    [Google Scholar]
  20. Matthijnssens J. , Heylen E. , Zeller M. , Rahman M. , Lemey P. , Van Ranst M. . ( 2010; ). Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. . Mol Biol Evol 27:, 2431–2436. [CrossRef] [PubMed]
    [Google Scholar]
  21. Matthijnssens J. , Ciarlet M. , McDonald S. M. , Attoui H. , Bányai K. , Brister J. R. , Buesa J. , Esona M. D. , Estes M. K. . & other authors ( 2011; ). Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG). . Arch Virol 156:, 1397–1413. [CrossRef] [PubMed]
    [Google Scholar]
  22. Miyazaki A. , Kuga K. , Suzuki T. , Kohmoto M. , Katsuda K. , Tsunemitsu H. . ( 2011; ). Genetic diversity of group A rotaviruses associated with repeated outbreaks of diarrhea in a farrow-to-finish farm: identification of a porcine rotavirus strain bearing a novel VP7 genotype, G26. . Vet Res 42:, 112. [CrossRef] [PubMed]
    [Google Scholar]
  23. Mladenova Z. , Papp H. , Lengyel G. , Kisfali P. , Steyer A. F. , Steyer A. F. , Esona M. D. , Iturriza-Gómara M. , Bányai K. . ( 2012; ). Detection of rare reassortant G5P[6] rotavirus, Bulgaria. . Infect Genet Evol 12:, 1676–1684. [CrossRef] [PubMed]
    [Google Scholar]
  24. Mukherjee A. , Dutta D. , Ghosh S. , Bagchi P. , Chattopadhyay S. , Nagashima S. , Kobayashi N. , Dutta P. , Krishnan T. . & other authors ( 2009; ). Full genomic analysis of a human group A rotavirus G9P[6] strain from Eastern India provides evidence for porcine-to-human interspecies transmission. . Arch Virol 154:, 733–746. [CrossRef] [PubMed]
    [Google Scholar]
  25. Mukherjee A. , Chattopadhyay S. , Bagchi P. , Dutta D. , Singh N. B. , Arora R. , Parashar U. D. , Gentsch J. R. , Chawla-Sarkar M. . ( 2010; ). Surveillance and molecular characterization of rotavirus strains circulating in Manipur, north-eastern India: increasing prevalence of emerging G12 strains. . Infect Genet Evol 10:, 311–320. [CrossRef] [PubMed]
    [Google Scholar]
  26. Mukherjee A. , Ghosh S. , Bagchi P. , Dutta D. , Chattopadhyay S. , Kobayashi N. , Chawla-Sarkar M. . ( 2011; ). Full genomic analyses of human rotavirus G4P[4], G4P[6], G9P[19] and G10P[6] strains from North-eastern India: evidence for interspecies transmission and complex reassortment events. . Clin Microbiol Infect 17:, 1343–1346.[PubMed] [CrossRef]
    [Google Scholar]
  27. Nguyen T. A. , Khamrin P. , Trinh Q. D. , Phan T. G. , Pham D. , Hoang P. , Hoang K. T. , Yagyu F. , Okitsu S. , Ushijima H. . ( 2007; ). Sequence analysis of Vietnamese P[6] rotavirus strains suggests evidence of interspecies transmission. . J Med Virol 79:, 1959–1965. [CrossRef] [PubMed]
    [Google Scholar]
  28. Santos N. , Hoshino Y. . ( 2005; ). Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. . Rev Med Virol 15:, 29–56. [CrossRef] [PubMed]
    [Google Scholar]
  29. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  30. Taniguchi K. , Wakasugi F. , Pongsuwanna Y. , Urasawa T. , Ukae S. , Chiba S. , Urasawa S. . ( 1992; ). Identification of human and bovine rotavirus serotypes by polymerase chain reaction. . Epidemiol Infect 109:, 303–312. [CrossRef] [PubMed]
    [Google Scholar]
  31. Taniguchi K. , Nishikawa K. , Kobayashi N. , Urasawa T. , Wu H. , Gorziglia M. , Urasawa S. . ( 1994; ). Differences in plaque size and VP4 sequence found in SA11 virus clones having simian authentic VP4. . Virology 198:, 325–330. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tate, J. E., Burton, A. H., Boschi-Pinto, C., Steele, A. D., Dugue, J., Parashar, U. D. & WHO-coordinated Global Rotavirus Surveillance Network (2012). 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis. Lancet Infect Dis 12, 136–141.
  33. Teodoroff T. A. , Tsunemitsu H. , Okamoto K. , Katsuda K. , Kohmoto M. , Kawashima K. , Nakagomi T. , Nakagomi O. . ( 2005; ). Predominance of porcine rotavirus G9 in Japanese piglets with diarrhea: close relationship of their VP7 genes with those of recent human G9 strains. . J Clin Microbiol 43:, 1377–1384. [CrossRef] [PubMed]
    [Google Scholar]
  34. Timenetsky Mdo. C. , Gouvea V. , Santos N. , Carmona R. C. , Hoshino Y. . ( 1997; ). A novel human rotavirus serotype with dual G5-G11 specificity. . J Gen Virol 78:, 1373–1378.[PubMed]
    [Google Scholar]
  35. Trojnar E. , Sachsenröder J. , Twardziok S. , Reetz J. , Otto P. H. , Johne R. . ( 2013; ). Identification of an avian group A rotavirus containing a novel VP4 gene with a close relationship to those of mammalian rotaviruses. . J Gen Virol 94:, 136–142. [CrossRef] [PubMed]
    [Google Scholar]
  36. Urasawa T. , Urasawa S. , Taniguchi K. . ( 1981; ). Sequential passages of human rotavirus in MA-104 cells. . Microbiol Immunol 25:, 1025–1035.[PubMed] [CrossRef]
    [Google Scholar]
  37. Wang Y.-H. , Kobayashi N. , Nagashima S. , Zhou X. , Ghosh S. , Peng J.-S. , Hu Q. , Zhou D.-J. , Yang Z.-Q. . ( 2010; ). Full genomic analysis of a porcine-bovine reassortant G4P[6] rotavirus strain R479 isolated from an infant in China. . J Med Virol 82:, 1094–1102. [CrossRef] [PubMed]
    [Google Scholar]
  38. Wu H. , Taniguchi K. , Wakasugi F. , Ukae S. , Chiba S. , Ohseto M. , Hasegawa A. , Urasawa T. , Urasawa S. . ( 1994; ). Survey on the distribution of the gene 4 alleles of human rotaviruses by polymerase chain reaction. . Epidemiol Infect 112:, 615–622. [CrossRef] [PubMed]
    [Google Scholar]
  39. Zeller M. , Heylen E. , De Coster S. , Van Ranst M. , Matthijnssens J. . ( 2012; ). Full genome characterization of a porcine-like human G9P[6] rotavirus strain isolated from an infant in Belgium. . Infect Genet Evol 12:, 1492–1500. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.051011-0
Loading
/content/journal/jgv/10.1099/vir.0.051011-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error