1887

Abstract

The human bocavirus 1 (HBoV1) parvovirus causes respiratory disease and primarily affects children. Despite its worldwide prevalence, the mechanisms of HBoV1 replication and pathogenesis remain largely undefined. In this study of 846 children hospitalized at the Children’s Hospital of Chongqing Medical University in China for respiratory tract infection between June 2009 and May 2011, HBoV1 was detected in 112 (13.2 %) by real-time quantitative PCR. The median age of HBoV1-positive patients was 10 months old. Forty-five (40.2 %) of the HBoV1 cases were monoinfections, and 67 (59.8 %) were viral co-infections. Genotyping of all 112 HBoV1-positive cases yielded 27 full HBoV1 sequences, as well as two NS1 gene sequences, 15 NP1 gene sequences and 10 VP1/VP2 gene sequences harbouring 24, 10 and 43 mutations, respectively. Statistical analysis revealed no relationship between genetic mutations and clinical manifestations of HBoV1-positive patients. However, the viral loads were significantly lower in samples with mutations G236A or A447G in NP1, or G1461A in VP1/VP2, than in samples with wild-type HBoV1. Future studies should investigate whether these mutations in the HBoV1 gene may represent useful markers of disease pathogenesis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.047472-0
2013-05-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/94/5/1079.html?itemId=/content/journal/jgv/10.1099/vir.0.047472-0&mimeType=html&fmt=ahah

References

  1. Allander T., Tammi M. T., Eriksson M., Bjerkner A., Tiveljung-Lindell A., Andersson B.. ( 2005;). Cloning of a human parvovirus by molecular screening of respiratory tract samples. . Proc Natl Acad Sci U S A 102:, 12891–12896. [CrossRef][PubMed]
    [Google Scholar]
  2. Allander T., Jartti T., Gupta S., Niesters H. G., Lehtinen P., Osterback R., Vuorinen T., Waris M., Bjerkner A.. & other authors ( 2007;). Human bocavirus and acute wheezing in children. . Clin Infect Dis 44:, 904–910. [CrossRef][PubMed]
    [Google Scholar]
  3. Chen A. Y., Cheng F., Lou S., Luo Y., Liu Z., Delwart E., Pintel D., Qiu J.. ( 2010;). Characterization of the gene expression profile of human bocavirus. . Virology 403:, 145–154. [CrossRef][PubMed]
    [Google Scholar]
  4. Chieochansin T., Chutinimitkul S., Payungporn S., Hiranras T., Samransamruajkit R., Theamboolers A., Poovorawan Y.. ( 2007;). Complete coding sequences and phylogenetic analysis of human bocavirus (HBoV). . Virus Res 129:, 54–57. [CrossRef][PubMed]
    [Google Scholar]
  5. Dijkman R., Koekkoek S. M., Molenkamp R., Schildgen O., van der Hoek L.. ( 2009;). Human bocavirus can be cultured in differentiated human airway epithelial cells. . J Virol 83:, 7739–7748. [CrossRef][PubMed]
    [Google Scholar]
  6. Erdman D. D., Durigon E. L., Wang Q. Y., Anderson L. J.. ( 1996;). Genetic diversity of human parvovirus B19: sequence analysis of the VP1/VP2 gene from multiple isolates. . J Gen Virol 77:, 2767–2774. [CrossRef][PubMed]
    [Google Scholar]
  7. Hemauer A., von Poblotzki A., Gigler A., Cassinotti P., Siegl G., Wolf H., Modrow S.. ( 1996;). Sequence variability among different parvovirus B19 isolates. . J Gen Virol 77:, 1781–1785. [CrossRef][PubMed]
    [Google Scholar]
  8. Jartti T., Hedman K., Jartti L., Ruuskanen O., Allander T., Söderlund-Venermo M.. ( 2012;). Human bocavirus-the first 5 years. . Rev Med Virol 22:, 46–64. [CrossRef][PubMed]
    [Google Scholar]
  9. Kaida A., Kubo H., Takakura K., Iritani N.. ( 2010;). Detection and quantitative analysis of human bocavirus associated with respiratory tract infection in Osaka City, Japan. . Microbiol Immunol 54:, 276–281.[PubMed]
    [Google Scholar]
  10. Kantola K., Hedman L., Allander T., Jartti T., Lehtinen P., Ruuskanen O., Hedman K., Söderlund-Venermo M.. ( 2008;). Serodiagnosis of human bocavirus infection. . Clin Infect Dis 46:, 540–546. [CrossRef][PubMed]
    [Google Scholar]
  11. Kantola K., Sadeghi M., Antikainen J., Kirveskari J., Delwart E., Hedman K., Söderlund-Venermo M.. ( 2010;). Real-time quantitative PCR detection of four human bocaviruses. . J Clin Microbiol 48:, 4044–4050. [CrossRef][PubMed]
    [Google Scholar]
  12. Körner R. W., Söderlund-Venermo M., van Koningsbruggen-Rietschel S., Kaiser R., Malecki M., Schildgen O.. ( 2011;). Severe human bocavirus infection, Germany. . Emerg Infect Dis 17:, 2303–2305. [CrossRef][PubMed]
    [Google Scholar]
  13. Lindner J., Zehentmeier S., Franssila R., Barabas S., Schroeder J., Deml L., Modrow S.. ( 2008;). CD4+ T helper cell responses against human bocavirus viral protein 2 virus like particles in healthy adults. . J Infect Dis 198:, 1677–1684. [CrossRef][PubMed]
    [Google Scholar]
  14. Lüsebrink J., Schildgen V., Tillmann R. L., Wittleben F., Böhmer A., Müller A., Schildgen O.. ( 2011;). Detection of head-to-tail DNA sequences of human bocavirus in clinical samples. . PLoS ONE 6:, e19457. [CrossRef][PubMed]
    [Google Scholar]
  15. Parekh S., Zoulim F., Ahn S. H., Tsai A., Li J., Kawai S., Khan N., Trépo C., Wands J., Tong S.. ( 2003;). Genome replication, virion secretion, and E antigen expression of naturally occurring hepatitis B virus core promoter mutants. . J Virol 77:, 6601–6612. [CrossRef][PubMed]
    [Google Scholar]
  16. Qu X. W., Liu W. P., Qi Z. Y., Duan Z. J., Zheng L. S., Kuang Z. Z., Zhang W. J., Hou Y. D.. ( 2008;). Phospholipase A2-like activity of human bocavirus VP1 unique region. . Biochem Biophys Res Commun 365:, 158–163. [CrossRef][PubMed]
    [Google Scholar]
  17. Shackelton L. A., Holmes E. C.. ( 2006;). Phylogenetic evidence for the rapid evolution of human B19 erythrovirus. . J Virol 80:, 3666–3669. [CrossRef][PubMed]
    [Google Scholar]
  18. Söderlund-Venermo M., Lahtinen A., Jartti T., Hedman L., Kemppainen K., Lehtinen P., Allander T., Ruuskanen O., Hedman K.. ( 2009;). Clinical assessment and improved diagnosis of bocavirus-induced wheezing in children, Finland. . Emerg Infect Dis 15:, 1423–1430. [CrossRef][PubMed]
    [Google Scholar]
  19. Streck A. F., Bonatto S. L., Homeier T., Souza C. K., Gonçalves K. R., Gava D., Canal C. W., Truyen U.. ( 2011;). High rate of viral evolution in the capsid protein of porcine parvovirus. . J Gen Virol 92:, 2628–2636. [CrossRef][PubMed]
    [Google Scholar]
  20. Sun Y., Chen A. Y., Cheng F., Guan W., Johnson F. B., Qiu J.. ( 2009;). Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. . J Virol 83:, 3956–3967. [CrossRef][PubMed]
    [Google Scholar]
  21. Takahashi N., Takada N., Hashimoto T., Okamoto T.. ( 1999;). Genetic heterogeneity of the immunogenic viral capsid protein region of human parvovirus B19 isolates obtained from an outbreak in a pediatric ward. . FEBS Lett 450:, 289–293. [CrossRef][PubMed]
    [Google Scholar]
  22. Tiveljung-Lindell A., Rotzén-Ostlund M., Gupta S., Ullstrand R., Grillner L., Zweygberg-Wirgart B., Allander T.. ( 2009;). Development and implementation of a molecular diagnostic platform for daily rapid detection of 15 respiratory viruses. . J Med Virol 81:, 167–175. [CrossRef][PubMed]
    [Google Scholar]
  23. Xiu W. Q., Liu G. H., Kang Y. L., Shen X. N., Xie J. F., Wang M. A., Zhang W. Q., Zheng K. C.. ( 2010;). Sequencing and phylogenetic analysis of the complete genome sequence of human bocavirus in Fuzhou, China. . Chinese J Zoonoses 26:, 158–162.
    [Google Scholar]
  24. Xu W., McDonough M. C., Erdman D. D.. ( 2000;). Species-specific identification of human adenoviruses by a multiplex PCR assay. . J Clin Microbiol 38:, 4114–4120.[PubMed]
    [Google Scholar]
  25. Zádori Z., Szelei J., Lacoste M. C., Li Y., Gariépy S., Raymond P., Allaire M., Nabi I. R., Tijssen P.. ( 2001;). A viral phospholipase A2 is required for parvovirus infectivity. . Dev Cell 1:, 291–302. [CrossRef][PubMed]
    [Google Scholar]
  26. Zhang Z., Zheng Z., Luo H., Meng J., Li H., Li Q., Zhang X., Ke X., Bai B.. & other authors ( 2012;). Human bocavirus NP1 inhibits IFN-β production by blocking association of IFN regulatory factor 3 with IFNB promoter. . J Immunol 189:, 1144–1153. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.047472-0
Loading
/content/journal/jgv/10.1099/vir.0.047472-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error