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Journal of General Virology header logo Journal of General Virology

Volume 93, Issue 5

Lytic Epstein–Barr virus infection in epithelial cells but not in B-lymphocytes is dependent on Blimp1 No Access

Abstract

Epstein–Barr virus (EBV) replicates in superficial differentiated cells of oral hairy leukoplakia (OHL). Differentiation of squamous epithelial cells depends on B-lymphocyte-induced maturation protein 1 (Blimp1). Here we show that expression of the EBV immediate-early protein BZLF1 is restricted to Blimp1-positive epithelial cells in OHL. Luciferase assays revealed Blimp1-dependent induction of the BZLF1 promoter Zp in epithelial cell lines. Expression of ZEB1, a negative regulator of Zp, and of Xbp-1, which mediates the Blimp1 effect on Zp in B-cells, was not affected by enforced Blimp1 expression. Moreover, Xbp-1 protein expression was not detected in differentiated epithelial cells of OHL. Thus, Blimp1 induces BZLF1 expression in epithelial cells independently of ZEB1 and Xbp-1. In contrast to epithelial cells of OHL, BZLF1 expression was also observed in Blimp1-negative lymphoid cells in infectious mononucleosis tonsils, suggesting that EBV replication in B-cells may be induced independently of terminal differentiation.

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© 2012 SGM
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/content/journal/jgv/10.1099/vir.0.038661-0
2012-05-01
2025-01-18
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/content/journal/jgv/10.1099/vir.0.038661-0
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Lytic Epstein–Barr virus infection in epithelial cells but not in B-lymphocytes is dependent on Blimp1
J. Gen. Virol. 93, 1059 (2012); https://doi.org/10.1099/vir.0.038661-0
/content/journal/jgv/10.1099/vir.0.038661-0
/content/journal/jgv/10.1099/vir.0.038661-0
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