1887

Abstract

Inoculation of the semi-persistent cauliflower mosaic virus (CaMV, genus ) is associated with successive brief (5–10 s) intracellular stylet punctures (pd) when aphids probe in epidermal and mesophyll cells. In contrast to non-persistent viruses, there is no evidence for which of the pd subphases (II-1, II-2 and II-3) is involved in the inoculation of CaMV. Experiments were conducted using the electrical penetration graph (EPG) technique to investigate which particular subphases of the pd are associated with the inoculation of CaMV to turnip by its aphid vector . In addition, the same aphid species/test plant combination was used to compare the role of the pd subphases in the inoculation of the non-persistent turnip mosaic virus (TuMV, genus ). Inoculation of TuMV was found to be related to subphase II-1, confirming earlier results, but CaMV inoculation appeared to be related exclusively to subphase II-2 instead. The mechanism of CaMV inoculation and the possible nature of subphase II-2 are discussed in the scope of our findings.

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2012-03-01
2020-10-26
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References

  1. Bradley R. H. E., Ganong R. Y.. 1955a; Some effects of formaldehyde on potato virus Y in vitro, and ability of aphids to transmit the virus when their stylets are treated with formaldehyde. Can J Microbiol1:783–793 [CrossRef][PubMed]
    [Google Scholar]
  2. Bradley R. H. E., Ganong R. Y.. 1955b; Evidence that potato virus Y is carried near the tip of the stylets of the aphid vector Myzus persicae (sulz.). Can J Microbiol1:775–782 [CrossRef][PubMed]
    [Google Scholar]
  3. Chalfant R. B., Chapman R. K.. 1962; Transmission of cabbage viruses A and B by the cabbage aphid and the green peach aphid. J Econ Entomol55:584–590[CrossRef]
    [Google Scholar]
  4. Collar J. L., Fereres A.. 1998; Nonpersistent virus transmission efficiency determined by aphid probing behavior during intracellular punctures. Environ Entomol27:583–591[CrossRef]
    [Google Scholar]
  5. Drucker M., Froissart R., Hébrard E., Uzest M., Ravallec M., Espérandieu P., Mani J. C., Pugnière M., Roquet F.. other authors 2002; Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector. Proc Natl Acad Sci U S A99:2422–2427 [CrossRef][PubMed]
    [Google Scholar]
  6. Fereres A., Moreno A.. 2009; Behavioural aspects influencing plant virus transmission by homopteran insects. Virus Res141:158–168 [CrossRef][PubMed]
    [Google Scholar]
  7. Fereres A., Pérez P., Gemeno C., Ponz F.. 1993; Transmission of Spanish pepper- and potato-PVY isolates by aphid (Homoptera: Aphididae) vectors: epidemiological implications. Environ Entomol22:1260–1265[CrossRef]
    [Google Scholar]
  8. Forbes A. R.. 1969; The stylets of the green peach aphid, Myzus persicae (Homoptera: Aphididae). Can Entomol101:31–41 [CrossRef]
    [Google Scholar]
  9. Franck A., Guilley H., Jonard G., Richards K., Hirth L.. 1980; Nucleotide sequence of cauliflower mosaic virus DNA. Cell21:285–294 [CrossRef][PubMed]
    [Google Scholar]
  10. Harris K. F.. 1977; An ingestion-egestion hypothesis of noncirculative virus transmission. In Aphids as Virus Vectors pp.165–220 Edited by Harris K. F., Maramorosch K.. New York: Academic Press;
    [Google Scholar]
  11. Martín B., Collar J. L., Tjallingii W. F., Fereres A.. 1997; Intracellular ingestion and salivation by aphids may cause the acquisition and inoculation of non-persistently transmitted plant viruses. J Gen Virol78:2701–2705[PubMed]
    [Google Scholar]
  12. McLean D. L., Kinsey M. G.. 1984; The precibarial valve and its role in the feeding behavior of the pea aphid, Acyrthosiphon pisum . Bull Entomol Soc Am30:26–31
    [Google Scholar]
  13. Moreno A., Palacios I., Blanc S., Fereres A.. 2005; Intracellular salivation is the mechanism involved in the inoculation of Cauliflower mosaic virus by its major vectors, Brevicoryne brassicae and Myzus persicae . Ann Entomol Soc Am98:763–769 [CrossRef]
    [Google Scholar]
  14. Moreno A., Garzo E., Fernandez-Mata G., Kassem M., Aranda M. A., Fereres A.. 2011; Aphids secrete watery saliva into plant tissues from the onset of stylet penetration. Entomol Exp Appl139:145–153 [CrossRef]
    [Google Scholar]
  15. Ng J. C. K., Falk B. W.. 2006; Virus–vector interactions mediating nonpersistent and semipersistent transmission of plant viruses. Annu Rev Phytopathol44:183–212 [CrossRef][PubMed]
    [Google Scholar]
  16. Palacios I., Drucker M., Blanc S., Leite S., Moreno A., Fereres A.. 2002; Cauliflower mosaic virus is preferentially acquired from the phloem by its aphid vectors. J Gen Virol83:3163–3171[PubMed]
    [Google Scholar]
  17. Powell G.. 2005; Intracellular salivation is the aphid activity associated with inoculation of non-persistently transmitted viruses. J Gen Virol86:469–472 [CrossRef][PubMed]
    [Google Scholar]
  18. Powell G., Pirone T., Hardie J.. 1995; Aphid stylet activities during potyvirus acquisition from plants and an in vitro system that correlate with subsequent transmission. Eur J Plant Pathol101:411–420 [CrossRef]
    [Google Scholar]
  19. Syller J.. 2005; The roles and mechanisms of helper component proteins encoded by potyviruses and caulimoviruses. Physiol Mol Plant Pathol67:119–130 [CrossRef]
    [Google Scholar]
  20. Sylvester E. S.. 1962; Mechanisms of plant virus transmission by aphids. In Symposium on Biological Transmission of Disease Agents pp.11–31 Edited by Maramorosch K.. New York: Academic Press;
    [Google Scholar]
  21. Tjallingii W. F.. 1985; Membrane potentials as an indication for plant cell penetration by aphid stylets. Entomol Exp Appl38:187–193 [CrossRef]
    [Google Scholar]
  22. Tjallingii W. F., Garzo E., Fereres A.. 2010; New structure in cell puncture activities by aphid stylets: a dual-mode EPG study. Entomol Exp Appl135:193–207 [CrossRef]
    [Google Scholar]
  23. Uzest M., Gargani D., Drucker M., Hébrard E., Garzo E., Candresse T., Fereres A., Blanc S.. 2007; A protein key to plant virus transmission at the tip of the insect vector stylet. Proc Natl Acad Sci U S A104:17959–17964 [CrossRef][PubMed]
    [Google Scholar]
  24. Uzest M., Gargani D., Dombrovsky A., Cazevieille C., Cot D., Blanc S.. 2010; The ‘acrostyle’: a newly described anatomical structure in aphid stylets. Arthropod Struct Dev39:221–229 [CrossRef][PubMed]
    [Google Scholar]
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