1887

Abstract

While performing a nationwide survey of hepatitis E virus (HEV) infection among 450 wild boars () that had been captured in Japan between November 2005 and March 2010, we found 16 boars (3.6 %) with ongoing HEV infection: 11 had genotype 3 HEV, four had genotype 4 HEV and the remaining boar was infected with HEV of an unrecognized genotype (designated wbJOY_06). The entire wbJOY_06 genome was sequenced and was found to comprise 7246 nt excluding the poly(A) tail. The wbJOY_06 isolate was highly divergent from known genotype 1–4 HEV isolates derived from humans, swine, wild boars, deer, mongoose and rabbits (=145) by 22.6–27.7 %, rat HEV isolates (=2) by 46.0–46.2 %, and avian HEV isolates (=5) by 52.5–53.1 % over the entire genome. A Simplot analysis revealed no significant recombination between the existing HEV strains of genotypes 1–4. Therefore, we propose that the wbJOY_06 isolate is the first member of a previously unidentified genotype.

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2011-04-01
2019-11-22
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vol. , part 4, pp. 902 - 908

Complete genome scanning carried out by the Simplot software program

An unrooted phylogenetic tree constructed by the neighbour-joining method

Comparison of the wbJOY_06 isolate of unclassifiable genotype with 152 reported mammalian and avian HEV isolates whose entire or near-entire sequence is known

HEV isolates used for comparative and phylogenetic analyses in the present study [Single PDF file](264 KB)

 



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