Here we report, for the first time, the nearly full-length genome sequence of porcine bocavirus (PBoV), a recently discovered parvovirus from pigs. Phylogenetic trees based on this genome sequence showed that PBoV belongs to the branch containing the genus , which comprises canine minute virus (CnMV), bovine parvovirus, gorilla bocavirus and human bocavirus (HBoV), and was most closely related to the group containing CnMV. PBoV was predicted to contain three potential ORFs encoding the non-structural protein NS1, the characteristic NP1 protein and the capsid protein VP1/VP2, with an organization similar to that of known bocaviruses. Interestingly, the gene of PBoV was more similar in length to the homogeneous gene found in HBoV than to those of other known bocaviruses. In addition, highly conserved unique splice-donor and -acceptor sites were identified in the gene of HBoV and PBoV.


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  1. Adlhoch, C., Kaiser, M., Ellerbrok, H. & Pauli, G.(2010). High prevalence of porcine Hokovirus in German wild boar populations. Virol J 7, 171. [CrossRef] [Google Scholar]
  2. Allander, T., Tammi, M. T., Eriksson, M., Bjerkner, A., Tiveljung-Lindell, A. & Andersson, B.(2005). Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A 102, 12891–12896. [CrossRef] [Google Scholar]
  3. Blomström, A. L., Belák, S., Fossum, C., McKillen, J., Allan, G., Wallgren, P. & Berg, M.(2009). Detection of a novel porcine boca-like virus in the background of porcine circovirus type 2 induced postweaning multisystemic wasting syndrome. Virus Res 146, 125–129. [CrossRef] [Google Scholar]
  4. Blomström, A. L., Belák, S., Fossum, C., Fuxler, L., Wallgren, P. & Berg, M.(2010). Studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. Virus Res 152, 59–64. [CrossRef] [Google Scholar]
  5. Chen, K. C., Shull, B. C., Moses, E. A., Lederman, M., Stout, E. R. & Bates, R. C.(1986). Complete nucleotide sequence and genome organization of bovine parvovirus. J Virol 60, 1085–1097. [Google Scholar]
  6. Chen, K. C., Shull, B. C., Lederman, M., Stout, E. R. & Bates, R. C.(1988). Analysis of the termini of the DNA of bovine parvovirus: demonstration of sequence inversion at the left terminus and its implication for the replication model. J Virol 62, 3807–3813. [Google Scholar]
  7. Chen, A. Y., Cheng, F., Lou, S., Luo, Y., Liu, Z., Delwart, E., Pintel, D. & Qiu, J.(2010). Characterization of the gene expression profile of human bocavirus. Virology 403, 145–154. [CrossRef] [Google Scholar]
  8. Cheung, A. K., Wu, G., Wang, D., Bayles, D. O., Lager, K. M. & Vincent, A. L.(2010). Identification and molecular cloning of a novel porcine parvovirus. Arch Virol 155, 801–806. [CrossRef] [Google Scholar]
  9. Kapoor, A., Mehta, N., Esper, F., Poljsak-Prijatelj, M., Quan, P. L., Qaisar, N., Delwart, E. & Lipkin, W. I.(2010a). Identification and characterization of a new bocavirus species in gorillas. PLoS ONE 5, e11948. [CrossRef] [Google Scholar]
  10. Kapoor, A., Simmonds, P., Slikas, E., Li, L., Bodhidatta, L., Sethabutr, O., Triki, H., Bahri, O., Oderinde, B. S. & other authors(2010b). Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections. J Infect Dis 201, 1633–1643. [CrossRef] [Google Scholar]
  11. Lau, S. K., Woo, P. C., Tse, H., Fu, C. T., Au, W. K., Chen, X. C., Tsoi, H. W., Tsang, T. H., Chan, J. S. & other authors(2008). Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4. J Gen Virol 89, 1840–1848. [CrossRef] [Google Scholar]
  12. Manteufel, J. & Truyen, U.(2008). Animal bocaviruses: a brief review. Intervirology 51, 328–334. [CrossRef] [Google Scholar]
  13. Qiu, J., Cheng, F., Johnson, F. B. & Pintel, D.(2007). The transcription profile of the bocavirus bovine parvovirus is unlike those of previously characterized parvoviruses. J Virol 81, 12080–12085. [CrossRef] [Google Scholar]
  14. Schwartz, D., Green, B., Carmichael, L. E. & Parrish, C. R.(2002). The canine minute virus (minute virus of canines) is a distinct parvovirus that is most similar to bovine parvovirus. Virology 302, 219–223. [CrossRef] [Google Scholar]
  15. Sun, Y., Chen, A. Y., Cheng, F., Guan, W., Johnson, F. B. & Qiu, J.(2009). Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. J Virol 83, 3956–3967. [CrossRef] [Google Scholar]
  16. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).MEGA4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599. [CrossRef] [Google Scholar]
  17. Tattersall, P., Bergoin, M., Bloom, M. E., Brown, K. E., Linden, R. M., Muzyczka, N., Parrish, C. R. & Tijsses, P.(2005). Family Parvoviridae. In Virus Taxonomy: Classification and Nomenclature of Viruses. Eighth report of the International Committee on the Taxonomy of Viruses, pp. 353–369. Edited by Fauquet, C. M., Mayo, M. A., Maniloff, J., Desselberger, U. & Ball, L. A.. London, UK. : Elsevier Academic Press. [Google Scholar]
  18. Wang, F., Wei, Y., Zhu, C., Huang, X., Xu, Y., Yu, L. & Yu, X.(2010). Novel parvovirus sublineage in the family of Parvoviridae. Virus Genes 41, 305–308. [CrossRef] [Google Scholar]
  19. Zhai, S., Yue, C., Wei, Z., Long, J., Ran, D., Lin, T., Deng, Y., Huang, L., Sun, L. & other authors(2010). High prevalence of a novel porcine bocavirus in weanling piglets with respiratory tract symptoms in China. Arch Virol 155, 1313–1317. [CrossRef] [Google Scholar]

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vol. , part 4, pp. 784 –788

. Primers used for PBoV complete coding-sequence analysis.

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