1887

Abstract

Oral inoculation is currently considered as the best approach to mimic natural TSE contamination in ruminants. In this study, we compared the timing of abnormal prion protein (PrP) dissemination and accumulation in the organism of susceptible sheep either orally inoculated or naturally infected with classical scrapie. Both animal groups shared a similar PrP dissemination scheme and accumulation dynamics in lymphoid tissues. However, orally challenged animals displayed an earlier neuro-invasion and a dramatically shorter incubation period than naturally exposed sheep. No differences were observed between the groups with regards to the neuro-invasion route. These results unambiguously indicate that oral inoculation can have an impact on both the earliness of neuro-invasion and the incubation period. They also support the statement that oral inoculation is a relevant model for investigating transmissible spongiform encephalopathy pathogenesis. Nevertheless, data obtained under such experimental conditions should be used with some caution.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.021311-0
2010-08-01
2020-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/91/8/2139.html?itemId=/content/journal/jgv/10.1099/vir.0.021311-0&mimeType=html&fmt=ahah

References

  1. Andréoletti O., Berthon P., Marc D., Sarradin P., Grosclaude J., van Keulen L., Schelcher F., Elsen J. M., Lantier F.. 2000; Early accumulation of PrPSc in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapie. J Gen Virol81:3115–3126
    [Google Scholar]
  2. Andréoletti O., Berthon P., Levavasseur E., Marc D., Lantier F., Monks E., Elsen J. M., Schelcher F.. 2002a; Phenotyping of protein-prion (PrPsc)-accumulating cells in lymphoid and neural tissues of naturally scrapie-affected sheep by double-labeling immunohistochemistry. J Histochem Cytochem50:1357–1370
    [Google Scholar]
  3. Andréoletti O., Lacroux C., Chabert A., Monnereau L., Tabouret G., Lantier F., Berthon P., Eychenne F., Lafond-Benestad S.. other authors 2002b; PrPSc accumulation in placentas of ewes exposed to natural scrapie: influence of foetal PrP genotype and effect on ewe-to-lamb transmission. J Gen Virol83:2607–2616
    [Google Scholar]
  4. Andréoletti O., Simon S., Lacroux C., Morel N., Tabouret G., Chabert A., Lugan S., Corbiere F., Ferre P.. other authors 2004; PrPSc accumulation in myocytes from sheep incubating natural scrapie. Nat Med10:591–593
    [Google Scholar]
  5. Baron T., Biacabe A. G., Arsac J. N., Benestad S., Groschup M. H.. 2007; Atypical transmissible spongiform encephalopathies (TSEs) in ruminants. Vaccine25:5625–5630
    [Google Scholar]
  6. Bellworthy S. J., Dexter G., Stack M., Chaplin M., Hawkins S. A., Simmons M. M., Jeffrey M., Martin S., Gonzalez L., Hill P.. 2005a; Natural transmission of BSE between sheep within an experimental flock. Vet Rec157:206
    [Google Scholar]
  7. Bellworthy S. J., Hawkins S. A., Green R. B., Blamire I., Dexter G., Dexter I., Lockey R., Jeffrey M., Ryder S.. & other authors (2005b). Tissue distribution of bovine spongiform encephalopathy infectivity in Romney sheep up to the onset of clinical disease after oral challenge. Vet Rec156:197–202
    [Google Scholar]
  8. Detwiler L. A., Baylis M.. 2003; The epidemiology of scrapie. Rev Sci Tech22:121–143
    [Google Scholar]
  9. Elsen J. M., Amigues Y., Schelcher F., Ducrocq V., Andréoletti O., Eychenne F., Khang J. V., Poivey J. P., Lantier F., Laplanche J. L.. 1999; Genetic susceptibility and transmission factors in scrapie: detailed analysis of an epidemic in a closed flock of Romanov. Arch Virol144:431–445
    [Google Scholar]
  10. Foster J. D., Bruce M., McConnell I., Chree A., Fraser H.. 1996; Detection of BSE infectivity in brain and spleen of experimentally infected sheep. Vet Rec138:546–548
    [Google Scholar]
  11. Foster J. D., Parnham D., Chong A., Goldmann W., Hunter N.. 2001a; Clinical signs, histopathology and genetics of experimental transmission of BSE and natural scrapie to sheep and goats. Vet Rec148:165–171
    [Google Scholar]
  12. Foster J. D., Parnham D. W., Hunter N., Bruce M.. 2001b; Distribution of the prion protein in sheep terminally affected with BSE following experimental oral transmission. J Gen Virol82:2319–2326
    [Google Scholar]
  13. Groschup M. H., Lacroux C., Buschmann A., Luhken G., Mathey J., Eiden M., Lugan S., Hoffmann C., Espinosa J. C.. other authors 2007; Classic scrapie in sheep with the ARR/ARR prion genotype in Germany and France. Emerg Infect Dis13:1201–1207
    [Google Scholar]
  14. Hoffmann C., Ziegler U., Buschmann A., Weber A., Kupfer L., Oelschlegel A., Hammerschmidt B., Groschup M. H.. 2007; Prions spread via the autonomic nervous system from the gut to the central nervous system in cattle incubating bovine spongiform encephalopathy. J Gen Virol88:1048–1055
    [Google Scholar]
  15. Jeffrey M., Begara-McGorum I., Clark S., Martin S., Clark J., Chaplin M., Gonzalez L.. 2002; Occurrence and distribution of infection-specific PrP in tissues of clinical scrapie cases and cull sheep from scrapie-affected farms in Shetland. J Comp Pathol127:264–273
    [Google Scholar]
  16. Konold T., Moore S. J., Bellworthy S. J., Simmons H. A.. 2008; Evidence of scrapie transmission via milk. BMC Vet Res4:14
    [Google Scholar]
  17. Lacroux C., Simon S., Benestad S. L., Maillet S., Mathey J., Lugan S., Corbiere F., Cassard H., Costes P.. other authors 2008; Prions in milk from ewes incubating natural scrapie. PLoS Pathog4:e1000238
    [Google Scholar]
  18. Lasmezas C. I., Cesbron J. Y., Deslys J. P., Demaimay R., Adjou K. T., Rioux R., Lemaire C., Locht C., Dormont D.. 1996; Immune system-dependent and -independent replication of the scrapie agent. J Virol70:1292–1295
    [Google Scholar]
  19. Ligios C., Cancedda M. G., Madau L., Santucciu C., Maestrale C., Agrimi U., Ru G., Di Guardo G.. 2006; PrPSc deposition in nervous tissues without lymphoid tissue involvement is frequently found in ARQ/ARQ Sarda breed sheep preclinically affected with natural scrapie. Arch Virol151:2007–2020
    [Google Scholar]
  20. Maignien T., Lasmezas C. I., Beringue V., Dormont D., Deslys J. P.. 1999; Pathogenesis of the oral route of infection of mice with scrapie and bovine spongiform encephalopathy agents. J Gen Virol80:3035–3042
    [Google Scholar]
  21. Ryder S. J., Dexter G. E., Heasman L., Warner R., Moore S. J.. 2009; Accumulation and dissemination of prion protein in experimental sheep scrapie in the natural host. BMC Vet Res5:9
    [Google Scholar]
  22. van Keulen L. J., Schreuder B. E., Vromans M. E., Langeveld J. P., Smits M. A.. 1999; Scrapie-associated prion protein in the gastrointestinal tract of sheep with natural scrapie. J Comp Pathol121:55–63
    [Google Scholar]
  23. van Keulen L. J., Schreuder B. E., Vromans M. E., Langeveld J. P., Smits M. A.. 2000; Pathogenesis of natural scrapie in sheep. Arch Virol Suppl16:57–71
    [Google Scholar]
  24. Vilotte J. L., Soulier S., Essalmani R., Stinnakre M. G., Vaiman D., Lepourry L., Da Silva J. C., Besnard N., Dawson M.. other authors 2001; Markedly increased susceptibility to natural sheep scrapie of transgenic mice expressing ovine PrP. J Virol75:5977–5984
    [Google Scholar]
  25. Wells G. A., Konold T., Arnold M. E., Austin A. R., Hawkins S. A., Stack M., Simmons M. M., Lee Y. H., Gavier-Widen D.. other authors 2007; Bovine spongiform encephalopathy: the effect of oral exposure dose on attack rate and incubation period in cattle. J Gen Virol88:1363–1373
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.021311-0
Loading
/content/journal/jgv/10.1099/vir.0.021311-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error