1887

Abstract

Geminiviruses of the genera and utilize three replication modes: complementary-strand replication (CSR), rolling-circle replication (RCR) and recombination-dependent replication (RDR). Using two-dimensional gel electrophoresis, we now show for the first time that maize streak virus (MSV), the type member of the most divergent geminivirus genus, , does the same. Although mastreviruses have fewer regulatory genes than other geminiviruses and uniquely express their replication-associated protein (Rep) from a spliced transcript, the replicative intermediates of CSR, RCR and RDR could be detected unequivocally within infected maize tissues. All replicative intermediates accumulated early and, to varying degrees, were already present in the shoot apex and leaves at different maturation stages. Relative to other replicative intermediates, those associated with RCR increased in prevalence during leaf maturation. Interestingly, in addition to RCR-associated DNA forms seen in other geminiviruses, MSV also apparently uses dimeric open circular DNA as a template for RCR.

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2010-04-01
2019-11-22
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References

  1. Alberter, B., Rezaian, A. M. & Jeske, H. ( 2005; ). Replicative intermediates of ToLCV and its satellite DNAs. Virology 331, 441–448.[CrossRef]
    [Google Scholar]
  2. Anderson, H. J., Vonarx, E. J., Pastushok, L., Nakagawa, M., Katafuchi, A., Gruz, P., Di Rubbo, A., Grice, D. M., Osmond, M. J. & other authors ( 2008; ). Arabidopsis thaliana Y-family DNA polymerase catalyses translesion synthesis and interacts functionally with PCNA2. Plant J 55, 895–908.[CrossRef]
    [Google Scholar]
  3. Gutierrez, C. ( 2002; ). Strategies for geminivirus DNA replication and cell cycle interference. Physiol Mol Plant Pathol 60, 219–230.[CrossRef]
    [Google Scholar]
  4. Jeske, H. ( 2007; ). Replication of geminiviruses and the use of rolling circle amplification for their diagnosis. In Tomato Yellow Leaf Curl Virus Disease, pp. 141–156. Edited by H. Czosnek. Dordrecht, The Netherlands: Springer.
  5. Jeske, H. ( 2009; ). Geminiviruses. In Torque Teno Virus: the Still Elusive Human Pathogens, pp. 185–226. Edited by H. zur Hausen & E.-M. de Villiers. Berlin: Springer.
  6. Jeske, H., Lütgemeier, M. & Preiss, W. ( 2001; ). Distinct DNA forms indicate rolling circle and recombination-dependent replication of Abutilon mosaic geminivirus. EMBO J 20, 6158–6167.[CrossRef]
    [Google Scholar]
  7. Jovel, J., Preiß, W. & Jeske, H. ( 2007; ). Characterization of DNA-intermediates of an arising geminivirus. Virus Res 130, 63–70.[CrossRef]
    [Google Scholar]
  8. Lefeuvre, P., Lett, J.-M., Varsani, A. & Martin, D. P. ( 2009; ). Widely conserved recombination patterns amongst single stranded DNA viruses. J Virol 83, 2697–2707.[CrossRef]
    [Google Scholar]
  9. Lucy, A. P., Boulton, M. I., Davies, J. W. & Maule, A. J. ( 1996; ). Tissue specificity of Zea mays infection by maize streak virus. Mol Plant Microbe Interact 9, 22–31.[CrossRef]
    [Google Scholar]
  10. Morilla, G., Krenz, B., Jeske, H., Bejarano, E. R. & Wege, C. ( 2004; ). Tête à tête of tomato yellow leaf curl virus (TYLCV) and tomato yellow leaf curl Sardinia virus (TYLCSV) in single nuclei. J Virol 78, 10715–10723.[CrossRef]
    [Google Scholar]
  11. Morilla, G., Castillo, A. G., Preiß, W., Jeske, H. & Bejarano, E. R. ( 2006; ). A versatile transreplication-based system to identify cellular proteins involved in geminivirus replication. J Virol 80, 3624–3633.[CrossRef]
    [Google Scholar]
  12. Owor, B. E., Martin, D. P., Shepherd, D. N., Edema, R., Monjane, A. L., Rybicki, E. P., Thomson, J. A. & Varsani, A. ( 2007; ). Genetic analysis of maize streak virus isolates from Uganda reveals widespread distribution of a recombinant variant. J Gen Virol 88, 3154–3165.[CrossRef]
    [Google Scholar]
  13. Padidam, M., Sawyer, S. & Fauquet, C. M. ( 1999; ). Possible emergence of new geminiviruses by frequent recombination. Virology 265, 218–225.[CrossRef]
    [Google Scholar]
  14. Pilartz, M. & Jeske, H. ( 2003; ). Mapping of Abutilon mosaic geminivirus minichromosomes. J Virol 77, 10808–10818.[CrossRef]
    [Google Scholar]
  15. Pinner, M. S., Medina, V., Plaskitt, K. A. & Markham, P. G. ( 1993; ). Viral inclusions in monocotyledons infected by maize streak and related geminiviruses. Plant Pathol 42, 75–87.[CrossRef]
    [Google Scholar]
  16. Preiss, W. & Jeske, H. ( 2003; ). Multitasking in replication is common among geminiviruses. J Virol 77, 2972–2980.[CrossRef]
    [Google Scholar]
  17. Shepherd, D. N., Martin, D. P., van der Walt, E., Dent, K., Varsani, A. & Rybicki, E. P. ( 2009; ). Maize streak virus: an old and complex ‘emerging' pathogen. Mol Plant Pathol 11, 1–12.
    [Google Scholar]
  18. Stanley, J., Bisaro, D. M., Briddon, R. W., Brown, J. K., Fauquet, C. M., Harrison, B. D., Rybicki, E. P. & Stenger, D. C. ( 2005; ). Family Geminiviridae. In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses, pp. 301–326. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London: Elsevier/Academic Press.
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