1887

Journal of General Virology

Volume 90, Issue 10

Molecular characterization of full-length genomic segment 2 of a bovine picobirnavirus (PBV) strain: evidence for high genetic diversity with genogroup I PBVs Free

Abstract

We report here the molecular characterization of a bovine genogroup I picobirnavirus strain RUBV-P detected from a 1-month-old diarrhoeic calf in eastern India. Sequence comparisons and phylogenetic analysis of a short stretch of gene segment 2 of RUBV-P revealed low nucleotide identities (51.2–64.9 %) with and distant genetic relatedness to other genogroup I picobirnaviruses. The complete gene segment 2 sequence of RUBV-P was obtained by the single primer amplification method with modifications. Gene segment 2 of RUBV-P was 1758 bp long, encoded a predicted protein of 554 aa and exhibited low nucleotide (58.1–58.8 %) and amino acid (51.3–55.4 %) identities with genogroup I human strains Hy005102 and 1-CHN-97. The 5′- and 3′-end nucleotide sequences, and the three motifs of RNA-dependent RNA polymerases of double-stranded RNA viruses, were conserved among these strains. Our findings suggested that bovine strain RUBV-P might be distinct from genogroup I picobirnaviruses of humans and other animals.

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2009-10-01
2021-10-17
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Molecular characterization of full-length genomic segment 2 of a bovine picobirnavirus (PBV) strain: evidence for high genetic diversity with genogroup I PBVs
J. Gen. Virol. 90, 2519 (2009); https://doi.org/10.1099/vir.0.013987-0
/content/journal/jgv/10.1099/vir.0.013987-0
/content/journal/jgv/10.1099/vir.0.013987-0
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