1887

Abstract

A novel RNA virus with a bipartite genome has been found associated with an emerging disease affecting Japanese holly fern (). Diseased Japanese holly fern plants showed a variety of foliar symptoms and reduction in size. The virus was transmitted by grafting, as well as through spores from an infected plant. Partially purified preparations of the virus from infected ferns contained quasi-spherical particles that ranged from 30 to 40 nm in diameter. Double-stranded RNA (dsRNA) analyses from diseased plants yielded two major molecules of approximately 6.2 and 3.0 kbp in size, together with three other dsRNAs ascertained to be the replicative forms of subgenomic RNAs. The organization of RNA1 of this novel virus resembles that of raspberry bushy dwarf virus (genus ), whereas the genomic RNA2 showed a distinct organization and evolutionary origin. Results of this study indicate that the virus detected in diseased ferns is an undescribed phytovirus, for which the name Japanese holly fern mottle virus (JHFMoV) is proposed. Furthermore, we postulate that JHFMoV has enough distinguishing features to represent the type species of a novel genus of plant viruses. Taking into account the original host of the virus, we propose the name for this taxon.

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2009-10-01
2019-11-17
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vol. , part 10, pp. 2542–2549

Graft transmission of Japanese holly fern virus.

(a) Diagrammatic representation of polyproteins encoded by RNAs1 of JHFMoV and RBDV. (b) Agarose-gel electrophoresis of RT-PCR products generated by two sets of primers designed based on the MTR sequences of JHFMoV and RBDV.

RT-PCR detection of JHFMoV in foliar tissue of Japanese holly fern specimens from different geographical origins.

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