1887

Abstract

To counteract plant defence mechanisms, plant viruses have evolved to encode RNA silencing suppressor (RSS) proteins. These proteins can be identified by a range of silencing suppressor assays. Here, we describe a simple method using beet necrotic yellow vein virus (BNYVV) that allows a rapid screening of RSS activity. The viral inoculum consisted of BNYVV RNA1, which encodes proteins involved in viral replication, and two BNYVV-derived replicons: rep3–P30, which expresses the movement protein P30 of tobacco mosaic virus, and rep5–X, which allows the expression of a putative RSS (X). This approach has been validated through the use of several known RSSs. Two potential candidates have been tested and we show that, in our system, the P13 protein of burdock mottle virus displays RSS activity while the P0 protein of cereal yellow dwarf virus-RPV does not.

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2009-10-01
2019-11-14
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References

  1. Bortolamiol, D., Pazhouhandeh, M., Marrocco, K., Genschik, P. & Ziegler-Graff, V. ( 2007; ). The polerovirus F box protein P0 targets ARGONAUTE1 to suppress RNA silencing. Curr Biol 17, 1615–1621.[CrossRef]
    [Google Scholar]
  2. Bouzoubaa, S. & Scheidecker, D. ( 1990; ). Infection of protoplasts with beet necrotic yellow vein virus RNA. In Proceedings of the First Symposium of the International Working Group on Plant Viruses with Fungal Vectors, pp. 45–48. Edited by R. Koenig. Stuttgart, Germany: Eugen Ulmer.
  3. Chiba, M., Reed, J. C., Prokhnevsky, A. I., Chapman, E. J., Mawassi, M., Koonin, E. V., Carrington, J. C. & Dolja, V. V. ( 2006; ). Diverse suppressors of RNA silencing enhance agroinfection by a viral replicon. Virology 346, 7–14.[CrossRef]
    [Google Scholar]
  4. D'Arcy, C. J. & Domier, L. L. ( 2005; ). Luteoviridae. In Eighth Report of the International Committee on Taxonomy of Viruses, pp. 891–900. Edited by C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger & L. A. Ball. London, UK: Elsevier.
  5. Deom, C. M., Lapidot, M. & Beachy, R. N. ( 1992; ). Plant virus movement proteins. Cell 69, 221–224.[CrossRef]
    [Google Scholar]
  6. Diaz-Pendon, J. A. & Ding, S. W. ( 2008; ). Direct and indirect roles of viral suppressors of RNA silencing in pathogenesis. Annu Rev Phytopathol 46, 303–326.[CrossRef]
    [Google Scholar]
  7. Dunoyer, P., Pfeffer, S., Fritsch, C., Hemmer, O., Voinnet, O. & Richards, K. E. ( 2002; ). Identification, subcellular localization and some properties of a cysteine-rich suppressor of gene silencing encoded by peanut clump virus. Plant J 29, 555–567.[CrossRef]
    [Google Scholar]
  8. Gilmer, D., Bouzoubaa, S., Hehn, A., Guilley, H., Richards, K. & Jonard, G. ( 1992; ). Efficient cell-to-cell movement of beet necrotic yellow vein virus requires 3′ proximal genes located on RNA 2. Virology 189, 40–47.[CrossRef]
    [Google Scholar]
  9. Jupin, I., Bouzoubaa, S., Richards, K., Jonard, G. & Guilley, H. ( 1990; ). Multiplication of beet necrotic yellow vein virus RNA 3 lacking a 3′ poly(A) tail is accompanied by reappearance of the poly(A) tail and a novel short U-rich tract preceding it. Virology 178, 281–284.[CrossRef]
    [Google Scholar]
  10. Kasschau, K. D., Xie, Z., Allen, E., Llave, C., Chapman, E. J., Krizan, K. A. & Carrington, J. C. ( 2003; ). P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction. Dev Cell 4, 205–217.[CrossRef]
    [Google Scholar]
  11. Lauber, E., Bleykasten-Grosshans, C., Erhardt, M., Bouzoubaa, S., Jonard, G., Richards, K. E. & Guilley, H. ( 1998; ). Cell-to-cell movement of beet necrotic yellow vein virus: I. Heterologous complementation experiments provide evidence for specific interactions among the triple gene block proteins. Mol Plant Microbe Interact 11, 618–625.[CrossRef]
    [Google Scholar]
  12. Lauber, E., Jonard, G., Richards, K. & Guilley, H. ( 2005; ). Nonregulated expression of TGBp3 of hordei-like viruses but not of potex-like viruses inhibits beet necrotic yellow vein virus cell-to-cell movement. Arch Virol 150, 1459–1467.[CrossRef]
    [Google Scholar]
  13. Lecellier, C. H., Dunoyer, P., Arar, K., Lehmann-Che, J., Eyquem, S., Himber, C., Saib, A. & Voinnet, O. ( 2005; ). A cellular microRNA mediates antiviral defense in human cells. Science 308, 557–560.[CrossRef]
    [Google Scholar]
  14. Li, F. & Ding, S. W. ( 2006; ). Virus counterdefense: diverse strategies for evading the RNA-silencing immunity. Annu Rev Microbiol 60, 503–531.[CrossRef]
    [Google Scholar]
  15. Mangwende, T., Wang, M. L., Borth, W., Hu, J., Moore, P. H., Mirkov, T. E. & Albert, H. H. ( 2009; ). The P0 gene of Sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities. Virology 384, 38–50.[CrossRef]
    [Google Scholar]
  16. Mayo, M. A. & Ziegler-Graff, V. ( 1996; ). Molecular biology of luteoviruses. Adv Virus Res 46, 413–460.
    [Google Scholar]
  17. Moissiard, G. & Voinnet, O. ( 2004; ). Viral suppression of RNA silencing in plants. Mol Plant Pathol 5, 71–82.[CrossRef]
    [Google Scholar]
  18. Navarro, L., Jay, F., Nomura, K., He, S. Y. & Voinnet, O. ( 2008; ). Suppression of the microRNA pathway by bacterial effector proteins. Science 321, 964–967.[CrossRef]
    [Google Scholar]
  19. Pazhouhandeh, M., Dieterle, M., Marrocco, K., Lechner, E., Berry, B., Brault, V., Hemmer, O., Kretsch, T., Richards, K. E. & other authors ( 2006; ). F-box-like domain in the polerovirus protein P0 is required for silencing suppressor function. Proc Natl Acad Sci U S A 103, 1994–1999.[CrossRef]
    [Google Scholar]
  20. Pfeffer, S., Dunoyer, P., Heim, F., Richards, K. E., Jonard, G. & Ziegler-Graff, V. ( 2002; ). P0 of beet Western yellows virus is a suppressor of posttranscriptional gene silencing. J Virol 76, 6815–6824.[CrossRef]
    [Google Scholar]
  21. Powers, J. G., Sit, T. L., Qu, F., Morris, T. J., Kim, K. H. & Lommel, S. A. ( 2008; ). A versatile assay for the identification of RNA silencing suppressors based on complementation of viral movement. Mol Plant Microbe Interact 21, 879–890.[CrossRef]
    [Google Scholar]
  22. Qiu, W., Park, J. W. & Scholthof, H. B. ( 2002; ). Tombusvirus P19-mediated suppression of virus-induced gene silencing is controlled by genetic and dosage features that influence pathogenicity. Mol Plant Microbe Interact 15, 269–280.[CrossRef]
    [Google Scholar]
  23. Qu, F. & Morris, T. J. ( 2005; ). Suppressors of RNA silencing encoded by plant viruses and their role in viral infections. FEBS Lett 579, 5958–5964.[CrossRef]
    [Google Scholar]
  24. Quillet, L., Guilley, H., Jonard, G. & Richards, K. ( 1989; ). In vitro synthesis of biologically active beet necrotic yellow vein virus RNA. Virology 172, 293–301.[CrossRef]
    [Google Scholar]
  25. Rush, C. M. ( 2003; ). Ecology and epidemiology of benyviruses and plasmodiophorid vectors. Annu Rev Phytopathol 41, 567–592.[CrossRef]
    [Google Scholar]
  26. Schmidlin, L., Link, D., Mutterer, J., Guilley, H. & Gilmer, D. ( 2005; ). Use of a Beet necrotic yellow vein virus RNA-5-derived replicon as a new tool for gene expression. J Gen Virol 86, 463–467.[CrossRef]
    [Google Scholar]
  27. Schmitt, C., Balmori, E., Jonard, G., Richards, K. E. & Guilley, H. ( 1992; ). In vitro mutagenesis of biologically active transcripts of beet necrotic yellow vein virus RNA 2: evidence that a domain of the 75-kDa readthrough protein is important for efficient virus assembly. Proc Natl Acad Sci U S A 89, 5715–5719.[CrossRef]
    [Google Scholar]
  28. Silhavy, D., Molnar, A., Lucioli, A., Szittya, G., Hornyik, C., Tavazza, M. & Burgyan, J. ( 2002; ). A viral protein suppresses RNA silencing and binds silencing-generated, 21- to 25-nucleotide double-stranded RNAs. EMBO J 21, 3070–3080.[CrossRef]
    [Google Scholar]
  29. Vanitharani, R., Chellappan, P., Pita, J. S. & Fauquet, C. M. ( 2004; ). Differential roles of AC2 and AC4 of cassava geminiviruses in mediating synergism and suppression of posttranscriptional gene silencing. J Virol 78, 9487–9498.[CrossRef]
    [Google Scholar]
  30. Vincent, J. R., Lister, R. M. & Larkins, B. A. ( 1991; ). Nucleotide sequence analysis and genomic organization of the NY-RPV isolate of barley yellow dwarf virus. J Gen Virol 72, 2347–2355.[CrossRef]
    [Google Scholar]
  31. Voinnet, O., Vain, P., Angell, S. & Baulcombe, D. C. ( 1998; ). Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA. Cell 95, 177–187.[CrossRef]
    [Google Scholar]
  32. Voinnet, O., Pinto, Y. M. & Baulcombe, D. C. ( 1999; ). Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proc Natl Acad Sci U S A 96, 14147–14152.[CrossRef]
    [Google Scholar]
  33. Zhang, X., Yuan, Y. R., Pei, Y., Lin, S. S., Tuschl, T., Patel, D. J. & Chua, N. H. ( 2006; ). Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense. Genes Dev 20, 3255–3268.[CrossRef]
    [Google Scholar]
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