1887

Abstract

Activation of the host gene is essential for the lytic replication of Epstein–Barr virus (EBV). is activated by Zta (BZLF1, ZEBRA). Zta interacts directly with DNA through a series of closely related Zta-response elements (ZREs). Here we dissect the mechanism used by Zta to interact with the promoter and identify a weak interaction with ZRE that is dependent on the distal part of ZRE. Furthermore, we demonstrate that the ability of Zta to interact with ZRE is enhanced at least tenfold by methylation. The ability of Zta to transactivate a reporter construct driven by the promoter can be enhanced by methylation. As the ability of Zta to interact with a methylated ZRE in the EBV genome correlates with its ability to activate the expression of the endogenous viral gene , this suggests that Zta may also have the capability to overturn epigenetic control of .

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2009-06-01
2019-11-18
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Effect of methylation and Zta expression on the activity of the promoter in U2OS cells. [PDF](31 KB)



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