1887

Abstract

The nucleocapsid protein of Nipah virus produced in assembled into herringbone-like particles. The amino- and carboxy-termini of the N protein were shortened progressively to define the minimum contiguous sequence involved in capsid assembly. The first 29 aa residues of the N protein are dispensable for capsid formation. The 128 carboxy-terminal residues do not play a role in the assembly of the herringbone-like particles. A region with amino acid residues 30–32 plays a crucial role in the formation of the capsid particle. Deletion of any of the four conserved hydrophobic regions in the N protein impaired capsid formation. Replacement of the central conserved regions with the respective sequences from the Newcastle disease virus restored capsid formation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.005710-0
2009-02-01
2020-10-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/90/2/392.html?itemId=/content/journal/jgv/10.1099/vir.0.005710-0&mimeType=html&fmt=ahah

References

  1. AbuBakar S., Chang L. Y., Ali A. R., Sharifah S. H., Yusoff K., Zamrod Z. 2004; Isolation and molecular identification of Nipah virus from pigs. Emerg Infect Dis 10:2228–2230 [CrossRef]
    [Google Scholar]
  2. Bankamp B., Horikami S. M., Thompson P. D., Huber M., Billeter M., Moyer S. A. 1996; Domains of the measles virus N protein required for binding to P protein and self-assembly. Virology 216:272–277 [CrossRef]
    [Google Scholar]
  3. Bhella D., Ralph A., Murphy L. B., Yeo R. P. 2002; Significant differences in nucleocapsid morphology within the Paramyxoviridae . J Gen Virol 83:1831–1839
    [Google Scholar]
  4. Bhella D., Ralph A., Yeo R. P. 2004; Conformational flexibility in recombinant measles virus nucleocapsids visualised by cryo-negative stain electron microscopy and real-space helical reconstruction. J Mol Biol 340:319–331 [CrossRef]
    [Google Scholar]
  5. Buchholz C. J., Sphener D., Drillien R., Neubert W. J., Homann H. E. 1993; The conserved N-terminal region of Sendai virus nucleocapsid protein NP is required for nucleocapsid assembly. J Virol 67:5803–5812
    [Google Scholar]
  6. Buchholz C. J., Retzler C., Homann H. E., Neubert W. J. 1994; The carboxy-terminal domain of Sendai virus nucleocapsid protein is involved in complex formation between phosphoprotein and nucleocapsid-like particles. Virology 204:770–776 [CrossRef]
    [Google Scholar]
  7. Chadha M. S., Comer J. A., Lowe L., Rota P. A., Rollin P. E., Bellini W. J., Ksiazek T. G., Mishra A. C. 2006; Nipah virus-associated encephalitis outbreak, Siliguri, India. Emerg Infect Dis 12:235–240 [CrossRef]
    [Google Scholar]
  8. Chan Y. P., Koh C. L., Lam S. K., Wang L. F. 2004; Mapping of domains responsible for nucleocapsid protein–phosphoprotein interaction of henipaviruses. J Gen Virol 85:1675–1684 [CrossRef]
    [Google Scholar]
  9. Chua K. B., Goh K. J., Wong K. T., Kamarulzaman A., Tan P. S., Ksiazek T. G., Zaki S. R., Paul G., Lam S. K., Tan C. T. 1999; Fatal encephalitis due to Nipah virus among pig-farmers in Malaysia. Lancet 354:1257–1259 [CrossRef]
    [Google Scholar]
  10. Chua K. B., Bellini W. J., Rota P. A., Harcourt B. H., Tamin A., Lam S. K., Ksiazek T. G., Rollin P. E., Zaki S. R. & other authors; 2000; Nipah virus: a recently emergent deadly paramyxovirus. Science 288:1432–1435 [CrossRef]
    [Google Scholar]
  11. Curran J., Homann H., Buchholz C., Rochat S., Neubert W., Kolakofsky D. 1993; The hypervariable C-terminal tail of the Sendai paramyxovirus nucleocapsid protein is required for template function but not for RNA encapsidation. J Virol 67:4358–4364
    [Google Scholar]
  12. Diederich S., Maisner A. 2007; Molecular characteristics of the Nipah virus glycoproteins. Ann N Y Acad Sci 1102:39–50 [CrossRef]
    [Google Scholar]
  13. Eaton B. T., Broder C. C., Middleton D., Wang L. F. 2006; Hendra and Nipah viruses: different and dangerous. Nat Rev Microbiol 4:23–35 [CrossRef]
    [Google Scholar]
  14. Harcourt B. H., Lowe L., Tamin A., Liu X., Bankamp B., Bowden N., Rollin P. E., Comer J. A., Ksiazek T. G. other authors 2005; Genetic characterization of Nipah virus. Bangladesh: 2004 Emerg Infect Dis 11:1594–1597 [CrossRef]
    [Google Scholar]
  15. Heggeness M. H., Scheid A., Choppin P. W. 1981; The relationship of conformational changes in the Sendai virus nucleocapsid to proteolytic cleavage of the NP polypeptide. Virology 114:555–562 [CrossRef]
    [Google Scholar]
  16. Holmes D. E., Moyer S. A. 2002; The phosphoprotein (P) binding site resides in the N terminus of the L polymerase subunit of Sendai virus. J Virol 76:3078–3083 [CrossRef]
    [Google Scholar]
  17. Hsu V. P., Hossain M. J., Parashar U. D., Ali M. M., Ksiazek T. G., Kuzmin I., Niezgoda M., Rupprecht C., Bresee J., Breiman R. F. 2004; Nipah virus encephalitis re-emergence, Bangladesh. Emerg Infect Dis 10:2082–2087 [CrossRef]
    [Google Scholar]
  18. Kho C. L., Tan W. S., Yusoff K. 2001; Sequence analysis of the nucleoprotein of a Newcastle disease virus heat resistant strain: comparison with other members of the Paramyxoviridae . J Biochem Mol Biol Biophys 5:463–471
    [Google Scholar]
  19. Kho C. L., Tan W. S., Tey B. T., Yusoff K. 2003; NDV nucleocapsid: self-assembly and length determination domains. J Gen Virol 84:2163–2168 [CrossRef]
    [Google Scholar]
  20. Krishnamurthy S., Samal S. K. 1998; Identification of regions of bovine respiratory syncytial virus N protein required for binding to P protein and self-assembly. J Gen Virol 79:1399–1403
    [Google Scholar]
  21. Lamb R. A., Kolakofsky D. 2001; Paramyxoviridae : the viruses and their replication. In Fields Virology , 4th edn. pp 1305–1340Edited by Knipe D. M., Howley P. M. Philadelphia: Williams & Wilkins;
    [Google Scholar]
  22. MacLellan K., Loney C., Yeo R. P., Bhella D. 2007; The 24-Angstrom structure of respiratory syncytial virus nucleocapsid protein-RNA decameric rings. J Virol 81:9519–9524 [CrossRef]
    [Google Scholar]
  23. Myers T. M., Smallwood S., Moyer S. A. 1999; Identification of nucleocapsid protein residues required for Sendai virus nucleocapsid formation and genome replication. J Gen Virol 80:1383–1391
    [Google Scholar]
  24. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491 [CrossRef]
    [Google Scholar]
  25. Seal B. S., Crawford J. M., Sellers H. S., Locke D. P., King D. J. 2002; Nucleotide sequence analysis of the Newcastle disease virus nucleocapsid protein gene and phylogenetic relationships among the Paramyxoviridae . Virus Res 83:119–129 [CrossRef]
    [Google Scholar]
  26. Szewczyk E., Nayak T., Oakley C. E., Edgerton H., Xiong Y., Taheri-Talesh N., Osmani S., Oakley B. R. 2007; Fusion PCR and gene targeting in Aspergillus nidulans . Nat Protoc 1:3111–3120 [CrossRef]
    [Google Scholar]
  27. Tan W. S., Ong S. T., Eshaghi M., Foo S. S., Yusoff K. 2004; Solubility, immunogenicity and physical properties of the nucleocapsid protein of Nipah virus produced in Escherichia coli . J Med Virol 73:105–112 [CrossRef]
    [Google Scholar]
  28. Wang L. F., Yu M., Hasson E., Pritchard L. I., Shiell B., Michalski W. P., Eaton B. T. 2000; The exceptionally large genome of Hendra virus: support for creation of a new genus within the family Paramyxoviridae . J Virol 74:9972–9979 [CrossRef]
    [Google Scholar]
  29. Wang L. F., Harcourt B. H., Yu M., Tamin A., Rota P. A., Bellini W. J., Eaton B. T. 2001; Molecular biology of Hendra and Nipah viruses. Microbes Infect 3:279–287 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.005710-0
Loading
/content/journal/jgv/10.1099/vir.0.005710-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error