1887

Abstract

After previous studies had shown that natural transposon insertion between the two homologous regions and of the genome of the Mexican (M) strain of Cydia pomonella granulovirus (CpGV-M) resulted in a loss of viral competitiveness, the function of these homologous regions was investigated. A CpGV-based bacmid (CpBAC) was constructed and mutants with deleted and palindromes (CpBAC) and a construct (CpBAC) with physically separated and repeats were generated to investigate their involvement in replication. Based on median lethal concentration (LC) and median survival time (ST) of the mutant viruses vCpBAC and vCpBAC it was found that the infectivity of both mutants for codling moth L. (Lep.: Tortricidae) larvae was not influenced compared with the parental virus vCpBAC. Co-infection experiments with vCpBAC and vCpBAC using different virus ratios revealed that vCpBAC was efficiently out-competed by vCpBAC during replication. These findings suggested that the separation of and resulted in a replication disadvantage of the mutant similar to the observation made in previous co-infection experiments using the transposon-carrying mutant CpGV-MCp5 and WT CpGV-M. It was concluded that the palindromes and may play a non-essential but co-functional role in the replication of CpGV-M.

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2015-09-01
2020-01-22
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