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Abstract

Puumala virus (PUUV, carried by ) co-circulates with Seewis virus (SWSV, carried by ) in Finland. While PUUV causes 1000–3000 nephropathia epidemica (NE) cases annually, the pathogenicity of SWSV to man is unknown. To study the prevalence of SWSV antibodies in hantavirus fever-like patients' sera, we used recombinant SWSV nucleocapsid (N) protein as the antigen in ELISA, immunofluorescence assay (IFA) and immunoblotting. While characterizing the recombinant SWSV N protein, we observed that a polyclonal rabbit antiserum against PUUV N protein cross-reacted with SWSV N protein and vice versa. We initially screened 486 (450 PUUV-seronegative and 36 PUUV-seropositive) samples sent to Helsinki University Hospital Laboratory for PUUV serodiagnosis during 2002 and 2007 in an SWSV N protein IgG ELISA. In total, 4.2  % (19/450) of the PUUV-seronegative samples were reactive in the SWSV N protein IgG ELISA and none of the tested samples [43 PUUV-seronegative (weakly reactive in the SWSV IgG ELISA) and 15 random] were reactive in the SWSV N protein IgM ELISA. None of the IgG reactions could be confirmed by IFA or immunoblotting. Furthermore, among the 36 PUUV-seropositive samples three were reactive in SWSV N protein IgG and ten in SWSV N protein IgM ELISA. One PUUV-seropositive sample reacted with SWSV N protein in IFA and four in immunoblotting. Finally, we applied competitive ELISA to confirm that the observed reactivity was due to cross-reactivity rather than a true SWSV response. In conclusion, no evidence of SWSV infection was found among the 486 samples studied; however, we did demonstrate that PUUV antiserum cross-reacted with shrew-borne hantavirus N protein.

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2015-07-01
2024-12-14
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References

  1. Bennett S.N., Gu S.H., Kang H.J., Arai S., Yanagihara R. (2014). Reconstructing the evolutionary origins and phylogeography of hantavirusesTrends Microbiol 22473482 [View Article][PubMed]. [Google Scholar]
  2. Carey D.E., Reuben R., Panicker K.N., Shope R.E., Myers R.M. (1971). Thottapalayam virus: a presumptive arbovirus isolated from a shrew in IndiaIndian J Med Res 5917581760[PubMed]. [Google Scholar]
  3. Chu Y.K., Rossi C., Leduc J.W., Lee H.W., Schmaljohn C.S., Dalrymple J.M. (1994). Serological relationships among viruses in the Hantavirus genus, family Bunyaviridae Virology 198196204 [View Article][PubMed]. [Google Scholar]
  4. Clement J., Maes P., Van Ranst M. (2014). Hemorrhagic fever with renal syndrome in the new, and hantavirus pulmonary syndrome in the old world: paradi(se)gm lost or regained?Virus Res 1875558 [View Article][PubMed]. [Google Scholar]
  5. Gavrilovskaya I.N., Apekina N.S., Myasnikov Yu.A., Bernshtein A.D., Ryltseva E.V., Gorbachkova E.A., Chumakov M.P. (1983). Features of circulation of hemorrhagic fever with renal syndrome (HFRS) virus among small mammals in the European U.S.S.RArch Virol 75313316 [View Article][PubMed]. [Google Scholar]
  6. Goeijenbier M., Hartskeerl R.A., Reimerink J., Verner-Carlsson J., Wagenaar J.F., Goris M.G., Martina B.E., Lundkvist A., Koopmans M., other authors. (2014). The hanta hunting study: underdiagnosis of Puumala hantavirus infections in symptomatic non-travelling leptospirosis-suspected patients in the Netherlands, in 2010 and April to November 2011Euro Surveill 1920878[PubMed]. [Google Scholar]
  7. Guo W.P., Lin X.D., Wang W., Tian J.H., Cong M.L., Zhang H.L., Wang M.R., Zhou R.H., Wang J.B., other authors. (2013). Phylogeny and origins of hantaviruses harbored by bats, insectivores, and rodentsPLoS Pathog 9e1003159[PubMed].[CrossRef] [Google Scholar]
  8. Hepojoki J., Strandin T., Lankinen H., Vaheri A. (2012). Hantavirus structure-molecular interactions behind the sceneJ Gen Virol 9316311644 [View Article][PubMed]. [Google Scholar]
  9. Hepojoki J., Vaheri A., Strandin T. (2014). The fundamental role of endothelial cells in hantavirus pathogenesisFront Microbiol 5727 [View Article][PubMed]. [Google Scholar]
  10. Hepojoki J., Kipar A., Korzyukov Y., Bell-Sakyi L., Vapalahti O., Hetzel U. (2015). Replication of boid inclusion body disease-associated arenaviruses is temperature sensitive in both boid and mammalian cellsJ Virol 8911191128 [View Article][PubMed]. [Google Scholar]
  11. Hetzel U., Sironen T., Laurinmäki P., Liljeroos L., Patjas A., Henttonen H., Vaheri A., Artelt A., Kipar A., other authors. (2013). Isolation, identification, and characterization of novel arenaviruses, the etiological agents of boid inclusion body diseaseJ Virol 871091810935 [View Article][PubMed]. [Google Scholar]
  12. Jonsson C.B., Figueiredo L.T., Vapalahti O. (2010). A global perspective on hantavirus ecology, epidemiology, and diseaseClin Microbiol Rev 23412441 [View Article][PubMed]. [Google Scholar]
  13. Kang H.J., Arai S., Hope A.G., Song J.W., Cook J.A., Yanagihara R. (2009). Genetic diversity and phylogeography of Seewis virus in the Eurasian common shrew in Finland and HungaryVirol J 6208 [View Article][PubMed]. [Google Scholar]
  14. Kaukinen P., Vaheri A., Plyusnin A. (2003). Mapping of the regions involved in homotypic interactions of Tula hantavirus N proteinJ Virol 771091010916 [View Article][PubMed]. [Google Scholar]
  15. Lähdevirta J., Savola J., Brummer-Korvenkontio M., Berndt R., Illikainen R., Vaheri A. (1984). Clinical and serological diagnosis of nephropathia epidemica, the mild type of haemorrhagic fever with renal syndromeJ Infect 9230238 [View Article][PubMed]. [Google Scholar]
  16. Lee H.W., Cho H.J. (1981). Electron microscope appearance of Hantaan virus, the causative agent of Korean haemorrhagic feverLancet 31710701072 [View Article][PubMed]. [Google Scholar]
  17. Li X.D., Mäkelä T.P., Guo D., Soliymani R., Koistinen V., Vapalahti O., Vaheri A., Lankinen H. (2002). Hantavirus nucleocapsid protein interacts with the Fas-mediated apoptosis enhancer DaxxJ Gen Virol 83759766[PubMed]. [Google Scholar]
  18. Ling J., Sironen T., Voutilainen L., Hepojoki S., Niemimaa J., Isoviita V.M., Vaheri A., Henttonen H., Vapalahti O. (2014). Hantaviruses in Finnish soricomorphs: evidence for two distinct hantaviruses carried by Sorex araneus suggesting ancient host-switchInfect Genet Evol 275161 [View Article][PubMed]. [Google Scholar]
  19. Lundkvist A., Fatouros A., Niklasson B. (1991). Antigenic variation of European haemorrhagic fever with renal syndrome virus strains characterized using bank vole monoclonal antibodiesJ Gen Virol 7220972103 [View Article][PubMed]. [Google Scholar]
  20. Lundkvist A., Vapalahti O., Plyusnin A., Sjölander K.B., Niklasson B., Vaheri A. (1996). Characterization of Tula virus antigenic determinants defined by monoclonal antibodies raised against baculovirus-expressed nucleocapsid proteinVirus Res 452944 [View Article][PubMed]. [Google Scholar]
  21. Macé G., Feyeux C., Mollard N., Chantegret C., Audia S., Rebibou J.M., Spagnolo G., Bour J.B., Denoyel G.A., other authors. (2013). Severe Seoul hantavirus infection in a pregnant woman, France, October 2012Euro Surveill 1820464[PubMed]. [Google Scholar]
  22. Plyusnin A., Sironen T. (2014). Evolution of hantaviruses: co-speciation with reservoir hosts for more than 100 MYRVirus Res 1872226 [View Article][PubMed]. [Google Scholar]
  23. Resman K., Korva M., Fajs L., Zidarič T., Trilar T., Zupanc T.A. (2013). Molecular evidence and high genetic diversity of shrew-borne Seewis virus in SloveniaVirus Res 177113117 [View Article][PubMed]. [Google Scholar]
  24. Saasa N., Yoshida H., Shimizu K., Sánchez-Hernández C., Romero-Almaraz M.L., Koma T., Sanada T., Seto T., Yoshii K., other authors. (2012). The N-terminus of the Montano virus nucleocapsid protein possesses broadly cross-reactive conformation-dependent epitopes conserved in rodent-borne hantavirusesVirology 4284857 [View Article][PubMed]. [Google Scholar]
  25. Schlegel M., Tegshduuren E., Yoshimatsu K., Petraityte R., Sasnauskas K., Hammerschmidt B., Friedrich R., Mertens M., Groschup M.H., other authors. (2012a). Novel serological tools for detection of Thottapalayam virus, a Soricomorpha-borne hantavirusArch Virol 15721792187 [View Article][PubMed]. [Google Scholar]
  26. Schlegel M., Radosa L., Rosenfeld U.M., Schmidt S., Triebenbacher C., Löhr P.W., Fuchs D., Heroldová M., Jánová E., other authors. (2012b). Broad geographical distribution and high genetic diversity of shrew-borne Seewis hantavirus in Central EuropeVirus Genes 454855 [View Article][PubMed]. [Google Scholar]
  27. Song J.W., Baek L.J., Schmaljohn C.S., Yanagihara R. (2007a). Thottapalayam virus, a prototype shrewborne hantavirusEmerg Infect Dis 13980985 [View Article][PubMed]. [Google Scholar]
  28. Song J.W., Gu S.H., Bennett S.N., Arai S., Puorger M., Hilbe M., Yanagihara R. (2007b). Seewis virus, a genetically distinct hantavirus in the Eurasian common shrew (Sorex araneus)Virol J 4114 [View Article][PubMed]. [Google Scholar]
  29. Tischler N.D., Rosemblatt M., Valenzuela P.D. (2008). Characterization of cross-reactive and serotype-specific epitopes on the nucleocapsid proteins of hantavirusesVirus Res 13519 [View Article][PubMed]. [Google Scholar]
  30. Vaheri A., Vapalahti O., Plyusnin A. (2008). How to diagnose hantavirus infections and detect them in rodents and insectivoresRev Med Virol 18277288 [View Article][PubMed]. [Google Scholar]
  31. Vaheri A., Strandin T., Hepojoki J., Sironen T., Henttonen H., Mäkelä S., Mustonen J. (2013). Uncovering the mysteries of hantavirus infectionsNat Rev Microbiol 11539550 [View Article][PubMed]. [Google Scholar]
  32. Vapalahti O., Plyusnin A., Vaheri A., Henttonen H. (1995a). Hantavirus antibodies in European mammalogistsLancet 3451569 [View Article][PubMed]. [Google Scholar]
  33. Vapalahti O., Kallio-Kokko H., Närvänen A., Julkunen I., Lundkvist A., Plyusnin A., Lehväslaiho H., Brummer-Korvenkontio M., Vaheri A., Lankinen H. (1995b). Human B-cell epitopes of Puumala virus nucleocapsid protein, the major antigen in early serological responseJ Med Virol 46293303 [View Article][PubMed]. [Google Scholar]
  34. Vapalahti O., Lundkvist A., Fedorov V., Conroy C.J., Hirvonen S., Plyusnina A., Nemirov K., Fredga K., Cook J.A., other authors. (1999). Isolation and characterization of a hantavirus from Lemmus sibiricus: evidence for host switch during hantavirus evolutionJ Virol 7355865592[PubMed]. [Google Scholar]
  35. Voutilainen L., Savola S., Kallio E.R., Laakkonen J., Vaheri A., Vapalahti O., Henttonen H. (2012). Environmental change and disease dynamics: effects of intensive forest management on Puumala hantavirus infection in boreal bank vole populationsPLoS ONE 7e39452 [View Article][PubMed]. [Google Scholar]
  36. Yashina L.N., Abramov S.A., Gutorov V.V., Dupal T.A., Krivopalov A.V., Panov V.V., Danchinova G.A., Vinogradov V.V., Luchnikova E.M., other authors. (2010). Seewis virus: phylogeography of a shrew-borne hantavirus in Siberia, RussiaVector Borne Zoonotic Dis 10585591 [View Article][PubMed]. [Google Scholar]
  37. Yoshimatsu K., Arikawa J. (2014a). Antigenic properties of N protein of hantavirusViruses 630973109 [View Article][PubMed]. [Google Scholar]
  38. Yoshimatsu K., Arikawa J. (2014b). Serological diagnosis with recombinant N antigen for hantavirus infectionVirus Res 1877783 [View Article][PubMed]. [Google Scholar]
  39. Yoshimatsu K., Arikawa J., Tamura M., Yoshida R., Lundkvist A., Niklasson B., Kariwa H., Azuma I. (1996). Characterization of the nucleocapsid protein of Hantaan virus strain 76-118 using monoclonal antibodiesJ Gen Virol 77695704 [View Article][PubMed]. [Google Scholar]
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