1887

Abstract

Lymphoma is the most common haematopoietic malignancy in dogs, but little is known about the aetiology of this heterogeneous group of cancers. In humans, the Epstein–Barr virus (EBV) is associated with several lymphoma subtypes. Recently, it was suggested that EBV or an EBV-like virus is circulating in dogs. We therefore investigated whether EBV, or a novel herpesvirus, is associated with canine lymphoma using both serological and molecular techniques. In an assay designed to detect antibodies to EBV viral capsid antigens, 41 % of dogs were positive. Dogs with cancers, including lymphoma, were more frequently positive than controls, but no particular association with B-cell lymphoma was noted. EBV-specific RNA and DNA sequences were not detected in lymphoma tissue by hybridization or PCR, and herpesvirus genomes were not detected using multiple degenerate PCR assays with the ability to detect novel herpesviruses. We therefore found no evidence that herpesviruses are directly involved in common types of canine lymphoma although cannot exclude the presence of an EBV-like virus in the canine population.

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2015-07-01
2019-12-13
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References

  1. Babcock G. J., Decker L. L., Volk M., Thorley-Lawson D. A.. ( 1998; ). EBV persistence in memory B cells in vivo. . Immunity 9:, 395–404. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bell A. J., Gallagher A., Mottram T., Lake A., Kane E. V., Lightfoot T., Roman E., Jarrett R. F.. ( 2014; ). Germ-line transmitted, chromosomally integrated HHV-6 and classical Hodgkin lymphoma. . PLoS One 9:, e112642. [CrossRef] [PubMed]
    [Google Scholar]
  3. Carbone A., Gloghini A.. ( 2008; ). KSHV/HHV8-associated lymphomas. . Br J Haematol 140:, 13–24.[PubMed]
    [Google Scholar]
  4. Chiou S. H., Chow K. C., Yang C. H., Chiang S. F., Lin C. H.. ( 2005; ). Discovery of Epstein–Barr virus (EBV)-encoded RNA signal and EBV nuclear antigen leader protein DNA sequence in pet dogs. . J Gen Virol 86:, 899–905. [CrossRef] [PubMed]
    [Google Scholar]
  5. Chiu H. C., Chow K. C., Fan Y. H., Chang S. C., Chiou S. H., Chiang S. F., Chiou C. H., Wu G. H., Yang H. C. et al. ( 2013; ). Expression of EBV-encoded oncogenes and EBV-like virions in multiple canine tumors. . Vet Microbiol 163:, 79–89. [CrossRef] [PubMed]
    [Google Scholar]
  6. Damania B.. ( 2004; ). Oncogenic gamma-herpesviruses: comparison of viral proteins involved in tumorigenesis. . Nat Rev Microbiol 2:, 656–668. [CrossRef] [PubMed]
    [Google Scholar]
  7. Ehlers B., Dural G., Yasmum N., Lembo T., de Thoisy B., Ryser-Degiorgis M. P., Ulrich R. G., McGeoch D. J.. ( 2008; ). Novel mammalian herpesviruses and lineages within the Gammaherpesvirinae: cospeciation and interspecies transfer. . J Virol 82:, 3509–3516. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gallagher A., Perry J., Shield L., Freeland J., MacKenzie J., Jarrett R. F.. ( 2002; ). Viruses and Hodgkin disease: no evidence of novel herpesviruses in non-EBV-associated lesions. . Int J Cancer 101:, 259–264. [CrossRef] [PubMed]
    [Google Scholar]
  9. Gallagher A., Perry J., Freeland J., Alexander F. E., Carman W. F., Shield L., Cartwright R., Jarrett R. F.. ( 2003; ). Hodgkin lymphoma and Epstein–Barr virus (EBV): no evidence to support hit-and-run mechanism in cases classified as non-EBV-associated. . Int J Cancer 104:, 624–630. [CrossRef] [PubMed]
    [Google Scholar]
  10. Gentilini F., Calzolari C., Turba M. E., Bettini G., Famigli-Bergamini P.. ( 2009; ). GeneScanning analysis of Ig/TCR gene rearrangements to detect clonality in canine lymphomas. . Vet Immunol Immunopathol 127:, 47–56. [CrossRef] [PubMed]
    [Google Scholar]
  11. Gulley M. L., Tang W.. ( 2008; ). Laboratory assays for Epstein–Barr virus-related disease. . J Mol Diagn 10:, 279–292. [CrossRef] [PubMed]
    [Google Scholar]
  12. Henle G., Henle W., Clifford P., Diehl V., Kafuko G. W., Kirya B. G., Klein G., Morrow R. H., Munube G. M. et al. ( 1969; ). Antibodies to Epstein–Barr virus in Burkitt’s lymphoma and control groups. . J Natl Cancer Inst 43:, 1147–1157.[PubMed]
    [Google Scholar]
  13. Huang S. H., Kozak P. J., Kim J., Habineza-Ndikuyeze G., Meade C., Gaurnier-Hausser A., Patel R., Robertson E., Mason N. J.. ( 2012; ). Evidence of an oncogenic gammaherpesvirus in domestic dogs. . Virology 427:, 107–117. [CrossRef] [PubMed]
    [Google Scholar]
  14. Jarrett R. F., Gallagher A., Gatherer D.. ( 2013; ). Molecular methods of virus detection in lymphoma. . Methods Mol Biol 971:, 277–293. [CrossRef] [PubMed]
    [Google Scholar]
  15. Merlo D. F., Rossi L., Pellegrino C., Ceppi M., Cardellino U., Capurro C., Ratto A., Sambucco P. L., Sestito V. et al. ( 2008; ). Cancer incidence in pet dogs: findings of the Animal Tumor Registry of Genoa, Italy. . J Vet Intern Med 22:, 976–984. [CrossRef] [PubMed]
    [Google Scholar]
  16. Milman G., Smith K. C., Erles K.. ( 2011; ). Serological detection of Epstein–Barr virus infection in dogs and cats. . Vet Microbiol 150:, 15–20. [CrossRef] [PubMed]
    [Google Scholar]
  17. Rezk S. A., Weiss L. M.. ( 2007; ). Epstein–Barr virus-associated lymphoproliferative disorders. . Hum Pathol 38:, 1293–1304. [CrossRef] [PubMed]
    [Google Scholar]
  18. Tischer B. K., Osterrieder N.. ( 2010; ). Herpesviruses – a zoonotic threat?. Vet Microbiol 140:, 266–270. [CrossRef] [PubMed]
    [Google Scholar]
  19. Troyer R. M., Beatty J. A., Stutzman-Rodriguez K. R., Carver S., Lozano C. C., Lee J. S., Lappin M. R., Riley S. P., Serieys L. E. et al. ( 2014; ). Novel gammaherpesviruses in North American domestic cats, bobcats, and pumas: identification, prevalence, and risk factors. . J Virol 88:, 3914–3924. [CrossRef] [PubMed]
    [Google Scholar]
  20. Vail D. M., MacEwen E. G.. ( 2000; ). Spontaneously occurring tumors of companion animals as models for human cancer. . Cancer Invest 18:, 781–792. [CrossRef] [PubMed]
    [Google Scholar]
  21. Young L. S., Rickinson A. B.. ( 2004; ). Epstein–Barr virus: 40 years on. . Nat Rev Cancer 4:, 757–768. [CrossRef] [PubMed]
    [Google Scholar]
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