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Volume 105, Issue 4

Oral administration of PEDV-dissolved Alg-CS gel induces high and sustained mucosal immunity in mice No Access

Abstract

Porcine epidemic diarrhea (PED) is a serious disease in piglets that leads to high mortality. An effective measure that provides higher IgA levels in the intestine and milk is required to decrease losses. Porcine epidemic diarrhea virus (PEDV) was dissolved in calcium alginate (Alg) and combined with chitosan (CS) via electrostatic interactions between cationic chitosan and anionic alginate to create a porous gel (Alg-CS+PEDV). The gel was used to immunize mice orally or in combination with subcutaneous injections of inactivated PEDV vaccine. At 12 and 24 days after immunization, levels of IgA and IgG in Alg-CS+PEDV were higher than with normal PEDV oral administration. At 24 days after immunization, the concentration of IFN-γ in Alg-CS+PEDV was higher than with normal PEDV oral administration. Furthermore, oral administration combining subcutaneous immunization induced higher levels of IgG and IgA than oral administration alone. Our study provides a new method for the preparation and administration of oral vaccines to achieve enhanced mucosal immunity against PEDV.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): calcium alginate-chitosan gel , mucosal IgA , oral administration and PEDV
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31972658)
    • Principle Award Recipient: SongYunfeng
  • National Key Research and Development Program of China (Award 2021YFD1800303)
    • Principle Award Recipient: YunfengSong
© 2024 The Authors
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/content/journal/jgv/10.1099/jgv.0.001979
2024-04-24
2024-05-04
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Oral administration of PEDV-dissolved Alg-CS gel induces high and sustained mucosal immunity in mice
J. Gen. Virol. 105, 001979 (2024); https://doi.org/10.1099/jgv.0.001979
/content/journal/jgv/10.1099/jgv.0.001979
/content/journal/jgv/10.1099/jgv.0.001979
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