1887

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Volume 105, Issue 4

Molecular and biological characterization of a bunyavirus infecting the brown planthopper () No Access

Abstract

A negative-strand symbiotic RNA virus, tentatively named Nilaparvata lugens Bunyavirus (NLBV), was identified in the brown planthopper (BPH, ). Phylogenetic analysis indicated that NLBV is a member of the genus (family , order ). Analysis of virus-derived small interfering RNA suggested that antiviral immunity of BPH was successfully activated by NLBV infection. Tissue-specific investigation showed that NLBV was mainly accumulated in the fat-body of BPH adults. Moreover, NLBV was detected in eggs of viruliferous female BPHs, suggesting the possibility of vertical transmission of NLBV in BPH. Additionally, no significant differences were observed for the biological properties between NLBV-infected and NLBV-free BPHs. Finally, analysis of geographic distribution indicated that NLBV may be prevalent in Southeast Asia. This study provided a comprehensive characterization on the molecular and biological properties of a symbiotic virus in BPH, which will contribute to our understanding of the increasingly discovered RNA viruses in insects.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bunyavirus , Nilaparvata lugens , symbiotic virus and virus derived small interfering RNA
Funding
This study was supported by the:
  • Ningbo Youth Leading Talent Program for Scientific and Technological Innovation (Award 2023QL035)
    • Principle Award Recipient: Jian-PingChen
  • National Natural Science Foundation of China (Award 32270150)
    • Principle Award Recipient: Qian-ZhuoMao
  • National Natural Science Foundation of China (Award 32270146)
    • Principle Award Recipient: Jun-MinLi
  • National Natural Science Foundation of China (Award U20A2036)
    • Principle Award Recipient: Jian-PingChen
© 2024 The Authors
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2024-04-11
2024-04-29
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Molecular and biological characterization of a bunyavirus infecting the brown planthopper (Nilaparvata lugens)
J. Gen. Virol. 105, 001977 (2024); https://doi.org/10.1099/jgv.0.001977
/content/journal/jgv/10.1099/jgv.0.001977
/content/journal/jgv/10.1099/jgv.0.001977
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