1887

Abstract

Negeviruses that infect insects are recently identified virus species that are phylogenetically related to several plant viruses. They exhibit a unique virion structure, an elliptical core with a short projection. Negeviruses encode two structural proteins, a glycoprotein that forms a short projection, and an envelope protein that forms an elliptical core. The glycoprotein has been reported only in the negeviruses’ genes, and not in phylogenetically related plant viruses’ genes. In this report, we first describe the three-dimensional electron cryo-microscopy (cryo-EM) structure of Tanay virus (TANAV), one of the nege-like viruses. TANAV particle demonstrates a periodical envelope structure consisting of three layers surrounding the centred viral RNA. The elliptical core dynamically changes its shape under acidic and even low detergent conditions to form bullet-like or tubular shapes. The further cryo-EM studies on these transformed TANAV particles reveal their overall structural rearrangement. These findings suggest putative geometries of TANAV and its transformation in the life cycle, and the potential importance of the short projection for enabling cell entry to the insect hosts.

Funding
This study was supported by the:
  • Collaborative Study Program of National Institute for Physiological Sciences (Award 2016-No. 38)
    • Principle Award Recipient: KentaOkamoto
  • Japan Society for the Promotion of Science (Award 25251009)
    • Principle Award Recipient: NaoyukiMIyazaki
  • Royal Swedish Academy of Sciences (Award BS2018-0053)
    • Principle Award Recipient: KentaOkamoto
  • Svenska Forskningsrådet Formas (Award 2018-00421)
    • Principle Award Recipient: KentaOkamoto
  • Swedish Foundation for International Cooperation in Research and Higher Education (Award JA2014-5721)
    • Principle Award Recipient: KentaOkamoto
  • Vetenskapsrådet (Award 2018-03387)
    • Principle Award Recipient: KentaOkamoto
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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/content/journal/jgv/10.1099/jgv.0.001863
2023-06-13
2024-06-14
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