1887

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Volume 103, Issue 12

Development of an homeostasis model between airway epithelial cells, bacteria and bacteriophages: a time-lapsed observation of cell viability and inflammatory response No Access

Abstract

Bacteriophages represent the most extensive group of viruses within the human virome and have a significant impact on general health and well-being by regulating bacterial population dynamics. , found in the anterior nostrils, throat and skin, is an opportunistic pathobiont that can cause a wide range of diseases, from chronic inflammation to severe and acute infections. In this study, we developed a human cell-based homeostasis model between a clinically isolated strain of 141 and active phages for this strain (PYO) isolated from the commercial Pyophage cocktail (PYO). The cocktail is produced by Eliava BioPreparations Ltd. (Tbilisi, Georgia) and is used as an add-on therapy for bacterial infections, mainly in Georgia. The triptych interaction model was evaluated by time-dependent analysis of cell death and inflammatory response of the nasal and bronchial epithelial cells. Inflammatory mediators (IL-8, CCL5/RANTES, IL-6 and IL-1β) in the culture supernatants were measured by enzyme-linked immunosorbent assay and cell viability was determined by crystal violet staining. By measuring trans-epithelial electrical resistance, we assessed the epithelial integrity of nasal cells that had differentiated under air-liquid interface conditions. PYO was found to have a prophylactic effect on airway epithelial cells exposed to 141 by effectively down-regulating bacterial-induced inflammation, cell death and epithelial barrier disruption in a time-dependent manner. Overall, the proposed model represents an advance in the way multi-component biological systems can be simulated .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): airway epithelium , bacteria , bacteriophages , time-lapsed analysis and triptych model
Funding
This study was supported by the:
  • HORIZON EUROPE Framework Programme (Award grant agreement No. 767015)
    • Principle Award Recipient: NotApplicable
© 2022 The Authors
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/content/journal/jgv/10.1099/jgv.0.001819
2022-12-19
2024-05-05
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Development of an in vitro homeostasis model between airway epithelial cells, bacteria and bacteriophages: a time-lapsed observation of cell viability and inflammatory response
J. Gen. Virol. 103, 001819 (2022); https://doi.org/10.1099/jgv.0.001819
/content/journal/jgv/10.1099/jgv.0.001819
/content/journal/jgv/10.1099/jgv.0.001819
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