1887

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Volume 103, Issue 12

Pseudorabies virus infection induces endoplasmic reticulum stress and unfolded protein response in suspension-cultured BHK-21 cells No Access

Abstract

Viral infections cause endoplasmic reticulum (ER) stress and subsequently unfolded protein response (UPR) which restores ER homeostasis. In this study, levels of proteins or transcription of three UPR pathways were examined in suspension-cultured BHK-21 cells to investigate Pseudorabies virus (PRV) infection-induced ER stress, in which glucose-related proteins 78 kD and 94 kD (GRP78 and GRP94) were upregulated. The downstream double-stranded RNA-activated protein kinase-like ER kinase (PERK) pathway was activated with upregulation of ATF4, CHOP, and GADD34, and the inositol requiring kinase 1 (IRE1) pathway was triggered by the splicing of X box-binding protein 1 (XBP1) mRNA and the enhanced expression of p58 and EDEM. Furthermore, our results showed that the ER stress, induced by 0.005 µM thapsigargin, promoted PRV replication in suspension-cultured BHK-21 cells, and that PRV glycoprotein B (gB) overexpression triggered the PERK and IRE1 pathways.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BHK-21 cells , endoplasmic reticulum stress , Pseudorabies virus and unfolded protein response
Funding
This study was supported by the:
  • National Key Research and Development Program of China (2022YFD1800800); Key Research and Development Program of Jiangsu Province (BE2022787)
© 2022 The Authors
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/content/journal/jgv/10.1099/jgv.0.001818
2022-12-19
2024-05-05
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Pseudorabies virus infection induces endoplasmic reticulum stress and unfolded protein response in suspension-cultured BHK-21 cells
J. Gen. Virol. 103, 001818 (2022); https://doi.org/10.1099/jgv.0.001818
/content/journal/jgv/10.1099/jgv.0.001818
/content/journal/jgv/10.1099/jgv.0.001818
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