1887

Abstract

The group A rotavirus (RVA) genome comprising 11 double-stranded RNAs encodes six structural proteins (VP1-VP4, VP6, and VP7) and six non-structural proteins (NSP1-NSP6). Among these 12 rotaviral proteins, NSP6 has been less studied as to its function. We previously prepared a recombinant NSP6-deficient RVA derived from simian strain SA11-L2 by reverse genetics, and found that the NSP6-deficient virus grew well in cell culture, although its growth was less abundant than that of the parental SA11-L2 strain. In this study, we examined the potency of a recombinant RVA incapable of NSP6 expression to cause diarrhoea in suckling mice. The suckling mice infected with the NSP6-deficient virus apparently experienced diarrhoea, although the symptom was milder and the duration of diarrhoea was shorter than in the mice infected with the authentic SA11-L2 strain. Thus, together with the results obtained for cultured cells in the previous study, it can be concluded that NSP6 is not necessarily required for replication and pathogenicity and .

Funding
This study was supported by the:
  • Takeda Science Foundation
    • Principle Award Recipient: SatoshiKomoto
  • Mochida Memorial Foundation for Medical and Pharmaceutical Research
    • Principle Award Recipient: SatoshiKomoto
  • Japan Society for the Promotion of Science (Award 21K08498)
    • Principle Award Recipient: SaoriFukuda
  • Japan Society for the Promotion of Science (Award 21K07057)
    • Principle Award Recipient: SatoshiKomoto
  • Japan Society for the Promotion of Science (Award 18K07150)
    • Principle Award Recipient: SatoshiKomoto
  • Japan Agency for Medical Research and Development (Award 21fk0108121h0602)
    • Principle Award Recipient: SatoshiKomoto
  • Japan Agency for Medical Research and Development (Award 20fk0108121h0601)
    • Principle Award Recipient: SatoshiKomoto
  • Japan Agency for Medical Research and Development (Award 19fk0108034h1103)
    • Principle Award Recipient: SatoshiKomoto
  • Japan Agency for Medical Research and Development (Award 18fk0108034h1102)
    • Principle Award Recipient: SatoshiKomoto
  • Japan Agency for Medical Research and Development (Award 18fk0108018h0403)
    • Principle Award Recipient: SatoshiKomoto
Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.001745
2022-05-31
2024-05-06
Loading full text...

Full text loading...

References

  1. Tate JE, Burton AH, Boschi-Pinto C, Parashar UD. n.d World health organization-coordinated global rotavirus surveillance network. global, regional, and national estimates of rotavirus mortality in children. Clin Infect Dis 1:S96–S105 [View Article]
    [Google Scholar]
  2. Mattion NM, Mitchell DB, Both GW, Estes MK. Expression of rotavirus proteins encoded by alternative open reading frames of genome segment 11. Virology 1991; 181:295–304 [View Article] [PubMed]
    [Google Scholar]
  3. Papa G, Borodavka A, Desselberger U. Viroplasm: Assembly and functions of rotavirus replication factories. Viruses 2021; 13:1349 [View Article] [PubMed]
    [Google Scholar]
  4. Rainsford EW, McCrae MA. Characterization of the NSP6 protein product of rotavirus gene 11. Virus Res 2007; 130:193–201 [View Article] [PubMed]
    [Google Scholar]
  5. Torres-Vega MA, González RA, Duarte M, Poncet D, López S et al. The C-terminal domain of rotavirus NSP5 is essential for its multimerization, hyperphosphorylation and interaction with NSP6. J Gen Virol 2000; 81:821–830 [View Article] [PubMed]
    [Google Scholar]
  6. González RA, Torres-Vega MA, López S, Arias CF. In vivo interaction among rotavirus nonstructural proteins. Arch Virol 1989; 143:981–996 [View Article] [PubMed]
    [Google Scholar]
  7. Viskovska M, Anish R, Hu L, Chow D-C, Hurwitz AM et al. Probing the sites of interactions of rotaviral proteins involved in replication. J Virol 2014; 88:12866–12881 [View Article] [PubMed]
    [Google Scholar]
  8. Holloway G, Johnson RI, Kang Y, Dang VT, Stojanovski D et al. Rotavirus NSP6 localizes to mitochondria via a predicted N-terminal-helix. J Gen Virol 2015; 96:3519–3524 [View Article] [PubMed]
    [Google Scholar]
  9. González SA, Burrone OR. Porcine OSU rotavirus segment 11 sequence shows common features with the viral gene of human origin. Nucleic Acids Res 1989; 17:6402 [View Article] [PubMed]
    [Google Scholar]
  10. Gorziglia M, Nishikawa K, Fukuhara N. Evidence of duplication in super short segment 11 of rabbit rotavirus Alabama strain. Virology 1989; 170:587–590 [View Article] [PubMed]
    [Google Scholar]
  11. Wu H, Taniguchi K, Urasawa T, Urasawa S. Serological and genomic characterization of human rotaviruses detected in China. J Med Virol 1998; 55:168–176 [View Article] [PubMed]
    [Google Scholar]
  12. Komoto S, Kanai Y, Fukuda S, Kugita M, Kawagishi T et al. Reverse genetics system demonstrates that rotavirus nonstructural protein NSP6 is not essential for viral replication in cell culture. J Virol 2017; 91:1–10 [View Article] [PubMed]
    [Google Scholar]
  13. Taniguchi K, Nishikawa K, Kobayashi N, Urasawa T, Wu H et al. Differences in plaque size and VP4 sequence found in SA11 virus clones having simian authentic VP4. Virology 1994; 198:325–330 [View Article] [PubMed]
    [Google Scholar]
  14. Kawahara T, Makizaki Y, Oikawa Y, Tanaka Y, Maeda A et al. Oral administration of Bifidobacterium bifidum G9-1 alleviates rotavirus gastroenteritis through regulation of intestinal homeostasis by inducing mucosal protective factors. PLoS One 2017; 12:e0173979 [View Article] [PubMed]
    [Google Scholar]
  15. Offit PA, Clark HF, Kornstein MJ, Plotkin SA. A murine model for oral infection with a primate rotavirus (simian SA11). J Virol 1984; 51:233–236 [View Article] [PubMed]
    [Google Scholar]
  16. Fukuda S, Hatazawa R, Kawamura Y, Yoshikawa T, Murata T et al. Rapid generation of rotavirus single-gene reassortants by means of eleven plasmid-only based reverse genetics. J Gen Virol 2020; 101:806–815 [View Article] [PubMed]
    [Google Scholar]
  17. Urasawa S, Urasawa T, Taniguchi K. Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains. Infect Immun 1982; 38:781–784 [View Article] [PubMed]
    [Google Scholar]
  18. Taniguchi K, Urasawa T, Urasawa S, Yasuhara T. Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to an enzyme-linked immunosorbent assay for subgroup determination. J Med Virol 1984; 14:115–125 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.001745
Loading
/content/journal/jgv/10.1099/jgv.0.001745
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error