1887

Abstract

The purification of virus particles is an essential process for the manufacture of vaccines. However, the application of different purification processes may affect the quality of the virus particles, such as structural integrity and homogeneity, which may further influence the infectivity and immunogenicity of the purified virus. In this study, we took Feline calicivirus (FCV), a common natural pathogen in cats belonging to , as a research model. By using cryo-electron microscopy (cryo-EM), we incorporated the 3D classification process as a virus flexibility evaluation system. Cryo-EM images of virus particles resulting from different purification processes were compared at near-atomic resolution. The results indicated that molecular sieving purification will impact the stability of P-domains through increasing flexibility as determined by the evaluation system, which can be extended to assess the purification effect on the entire particle. This evaluation process can be further applied to all non-enveloped viruses.

Funding
This study was supported by the:
  • Natural Science Foundation of Hebei Province (Award 19226631D)
    • Principle Award Recipient: GengMeng
  • National Natural Science Foundation of China (Award 32100156)
    • Principle Award Recipient: YuepingZhang
  • National Key Research and Development Program of China (Award 2019YFC1604600)
    • Principle Award Recipient: GengMeng
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/content/journal/jgv/10.1099/jgv.0.001742
2022-05-17
2022-07-06
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