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Volume 102, Issue 10

The role of interferon regulatory factor 7 in the pathogenicity and immunogenicity of rabies virus in a mouse model No Access

Abstract

Rabies is a zoonotic disease caused by the rabies virus (RABV). RABV can lead to fatal encephalitis and is still a serious threat in most parts of the world. Interferon regulatory factor 7 (IRF7) is the main transcriptional regulator of type I IFN, and it is crucial for the induction of IFNα/β and the type I IFN-dependent immune response. In this study, we focused on the role of IRF7 in the pathogenicity and immunogenicity of RABV using an IRF7 mouse model. The results showed that the absence of IRF7 made mice more susceptible to RABV, because IRF7 restricted the replication of RABV in the early stage of infection. IRF7 deficiency affected the recruitment of plasmacytoid dendritic cells to the draining lymph nodes (dLNs), reduced the production of type I IFN and expression of IFN-stimulated genes. Furthermore, we found that the ability to produce specific RABV-neutralizing antibody was impaired in IRF7 mice. Consistently, IRF7 deficiency affected the recruitment of germinal-centre B cells to dLNs, and the generation of plasma cells and RABV-specific antibody secreting cells. Moreover, the absence of IRF7 downregulated the induction of IFN-γ and reduced type 1 T helper cell (Th1)-dependent antibody production. Collectively, our findings demonstrate that IRF7 promotes humoral immune responses and compromises the pathogenicity of RABV in a mouse model.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): humoral immunity , IRF7 , pathogenicity , rabies virus and Th1
Funding
This study was supported by the:
  • national natural science foundation of china (Award 31522057 and 31872451)
    • Principle Award Recipient: LingZhao
© 2021 The Authors
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/content/journal/jgv/10.1099/jgv.0.001665
2021-10-18
2024-05-12
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The role of interferon regulatory factor 7 in the pathogenicity and immunogenicity of rabies virus in a mouse model
J. Gen. Virol. 102, 001665 (2021); https://doi.org/10.1099/jgv.0.001665
/content/journal/jgv/10.1099/jgv.0.001665
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