1887

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Volume 102, Issue 10

RNA virome diversity and infection in individual flies Open Access

Abstract

The endosymbiont bacteria of the genus are associated with multiple mutualistic effects on insect biology, including nutritional and antiviral properties. Members of the genus naturally occur in fly species of the genus , providing an operational model host for studying how virome composition may be affected by its presence. populations can carry a variety of strains of members of the genus , with the Au strain associated with strong antiviral protection under experimental conditions. We used sampled from the Perth Hills, Western Australia, to investigate the potential virus protective effect of the Au strain of on individual wild-caught flies. Our data revealed no appreciable variation in virus composition and abundance between individuals infected or uninfected with associated with the presence or absence of Au. However, it remains unclear whether Au might affect viral infection and host survival by increasing tolerance rather than inducing complete resistance. These data also provide new insights into the natural virome diversity of . Despite the small number of individuals sampled, we identified a repertoire of RNA viruses, including nora virus, galbut virus, thika virus and La Jolla virus, that have been identified in other species of the genus . Chaq virus-like sequences associated with galbut virus were also detected. In addition, we identified five novel viruses from the families , , and . Overall, this study highlights the complex interaction between and RNA virus infections and provides a baseline description of the natural virome of .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Drosophila simulans , evolution , meta-transcriptomics , phylogeny , RNA virome and Wolbachia
Funding
This study was supported by the:
  • national health and medical research council (Award GNT1103804)
    • Principle Award Recipient: AryA Hoffmann
  • national health and medical research counci (Award 1118640)
    • Principle Award Recipient: AryA Hoffmann
  • australian research council (Award FL170100022)
    • Principle Award Recipient: EdwardC. Holmes
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-10-27
2024-05-06
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RNA virome diversity and Wolbachia infection in individual Drosophila simulans flies
J. Gen. Virol. 102, 001639 (2021); https://doi.org/10.1099/jgv.0.001639
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