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Journal of General Virology header logo Journal of General Virology

Volume 100, Issue 10

Cellular microRNA miR-c89 inhibits replication of porcine reproductive and respiratory syndrome virus by targeting the host factor porcine retinoid X receptor β No Access

Abstract

MicroRNAs (miRNAs) play critical roles in the complex networks of virus–host interactions. Our previous research showed that porcine reproductive and respiratory syndrome virus (PRRSV) infection markedly upregulates miR-c89 expression, suggesting that miR-c89 may play an important role in PRRSV infection. The present study sought to determine the function of miR-c89 and its molecular mechanism during PRRSV infection. Using quantitative reverse transcription PCR (RT-qPCR) verification, we demonstrated that both highly pathogenic PRRSV and low-pathogenic PRRSV infection induced miR-c89 expression. The overexpression of miR-c89 significantly suppressed the replication of a variety of PRRSV strains, regardless of the timing of infection. Further, miR-c89 can directly target the 3′UTR of porcine retinoid X receptor β (RXRB) mRNA in a sequence-specific manner. Knockdown affected RXRB expression, as siRNA can suppress the replication of a variety of PRRSV strains. This work not only provides new insights into PRRSV–cell interactions, but also highlights the potential for the use of miR-c89 in the development of new antiviral strategies to combat PRRSV infection.

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Keyword(s): miRNA , post-transcriptional regulation , PRRSV , RXRB and virus–cell interaction
© 2019 The Authors
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/content/journal/jgv/10.1099/jgv.0.001320
2019-09-03
2025-12-14

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/content/journal/jgv/10.1099/jgv.0.001320
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Cellular microRNA miR-c89 inhibits replication of porcine reproductive and respiratory syndrome virus by targeting the host factor porcine retinoid X receptor β
J. Gen. Virol. 100, 1407 (2019); https://doi.org/10.1099/jgv.0.001320
/content/journal/jgv/10.1099/jgv.0.001320
/content/journal/jgv/10.1099/jgv.0.001320
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