1887

Abstract

Our investigation of 1004 faecal specimens from European bats for picornaviruses by broadly reactive nested reverse transcription-PCR found picornaviral RNA in 28 samples (2.8 %). Phylogenetic analysis of the partial 3D genomic region suggested that one bat virus belonged to the species Enterovirus G (EV-G, formerly Porcine enterovirus B). Bat infection was supported by relatively high EV-G concentrations of 1.1×10 RNA copies per gram of faeces. All other bat viruses belonged either to the bat-associated genus Mischivirus, or to an unclassified Picornaviridae group distantly related to the genus Sapelovirus. Members of this unclassified sapelovirus-related group had RNA secondary structures in their 3′-nontranslated regions that were typical of enteroviruses and that resembled structures that occur in bat-associated coronaviruses, suggesting ancient recombination events. Based on sequence distances, several picornaviruses from European and Chinese bats were likely conspecific, suggesting connectivity of virus populations. Due to their high mutation rates and their diversity, picornaviruses may be useful tools for studies of bat and virus ecology.

Keyword(s): bat , ecology , emerging viruses and taxonomy
Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000760
2017-05-30
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/98/5/955.html?itemId=/content/journal/jgv/10.1099/jgv.0.000760&mimeType=html&fmt=ahah

References

  1. Calisher CH, Childs JE, Field HE, Holmes KV, Schountz T. Bats: important reservoir hosts of emerging viruses. Clin Microbiol Rev 2006;19:531–545 [CrossRef][PubMed]
    [Google Scholar]
  2. Brook CE, Dobson AP. Bats as 'special' reservoirs for emerging zoonotic pathogens. Trends Microbiol 2015;23:172–180 [CrossRef][PubMed]
    [Google Scholar]
  3. Lau SK, Woo PC, Lai KK, Huang Y, Yip CC et al. Complete genome analysis of three novel picornaviruses from diverse bat species. J Virol 2011;85:8819–8828 [CrossRef][PubMed]
    [Google Scholar]
  4. Li L, Victoria JG, Wang C, Jones M, Fellers GM et al. Bat guano virome: predominance of dietary viruses from insects and plants plus novel mammalian viruses. J Virol 2010;84:6955–6965 [CrossRef][PubMed]
    [Google Scholar]
  5. Wu Z, Ren X, Yang L, Hu Y, Yang J et al. Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces. J Virol 2012;86:10999–11012 [CrossRef][PubMed]
    [Google Scholar]
  6. Kemenesi G, Zhang D, Marton S, Dallos B, Görföl T et al. Genetic characterization of a novel picornavirus detected in Miniopterus schreibersii bats. J Gen Virol 2015;96:815–821 [CrossRef][PubMed]
    [Google Scholar]
  7. Drexler JF, Corman VM, Müller MA, Maganga GD, Vallo P et al. Bats host major mammalian paramyxoviruses. Nat Commun 2012;3:796 [CrossRef][PubMed]
    [Google Scholar]
  8. Drexler JF, Baumgarte S, Luna LK, Stöcker A, Almeida PS et al. Genomic features and evolutionary constraints in Saffold-like cardioviruses. J Gen Virol 2010;91:1418–1427 [CrossRef][PubMed]
    [Google Scholar]
  9. Knowles NJ, Hovi T, Hyypia T, King AMQ, Lindberg AM et al. Family Picornaviridae. In King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ. (editors) Virus Taxonomy Classification and Nomenclature of Viruses Ninth Report of the International Committee on Taxonomy of Viruses San Diego: Elsevier; 2012; pp.855–880
    [Google Scholar]
  10. Drexler JF, Baumgarte S, de Souza Luna LK, Eschbach-Bludau M, Lukashev AN et al. Aichi virus shedding in high concentrations in patients with acute diarrhea. Emerg Infect Dis 2011;17:1544–1548 [CrossRef][PubMed]
    [Google Scholar]
  11. Stöcker A, Souza BF, Ribeiro TC, Netto EM, Araujo LO et al. Cosavirus infection in persons with and without gastroenteritis, Brazil. Emerg Infect Dis 2012;18:656–659 [CrossRef][PubMed]
    [Google Scholar]
  12. Lau SK, Woo PC, Yip CC, Choi GK, Wu Y et al. Identification of a novel feline picornavirus from the domestic cat. J Virol 2012;86:395–405 [CrossRef][PubMed]
    [Google Scholar]
  13. Woo PC, Lau SK, Choi GK, Yip CC, Huang Y et al. Complete genome sequence of a novel picornavirus, canine picornavirus, discovered in dogs. J Virol 2012;86:3402–3403 [CrossRef][PubMed]
    [Google Scholar]
  14. Pilipenko EV, Maslova SV, Sinyakov AN, Agol VI. Towards identification of cis-acting elements involved in the replication of enterovirus and rhinovirus RNAs: a proposal for the existence of tRNA-like terminal structures. Nucleic Acids Res 1992;20:1739–1745 [CrossRef][PubMed]
    [Google Scholar]
  15. Pilipenko EV, Poperechny KV, Maslova SV, Melchers WJ, Slot HJ et al. Cis-element, oriR, involved in the initiation of (-) strand poliovirus RNA: a quasi-globular multi-domain RNA structure maintained by tertiary ('kissing') interactions. EMBO J 1996;15:5428–5436[PubMed]
    [Google Scholar]
  16. Martin DP, Murrell B, Golden M, Khoosal A, Muhire B. RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 2015;1:vev003 [CrossRef][PubMed]
    [Google Scholar]
  17. Jonassen CM, Jonassen TO, Grinde B. A common RNA motif in the 3′ end of the genomes of astroviruses, avian infectious bronchitis virus and an equine rhinovirus. J Gen Virol 1998;79:715–718 [CrossRef][PubMed]
    [Google Scholar]
  18. Robertson MP, Igel H, Baertsch R, Haussler D, Ares M Jr et al. The structure of a rigorously conserved RNA element within the SARS virus genome. PLoS Biol 2005;3:e5 [CrossRef][PubMed]
    [Google Scholar]
  19. Huang C, Liu WJ, Xu W, Jin T, Zhao Y et al. A bat-derived putative cross-family recombinant coronavirus with a reovirus gene. PLoS Pathog 2016;12:e1005883 [CrossRef][PubMed]
    [Google Scholar]
  20. Sanjuán R. From molecular genetics to phylodynamics: evolutionary relevance of mutation rates across viruses. PLoS Pathog 2012;8:e1002685 [CrossRef][PubMed]
    [Google Scholar]
  21. Drexler JF, Gloza-Rausch F, Glende J, Corman VM, Muth D et al. Genomic characterization of severe acute respiratory syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences. J Virol 2010;84:11336–11349 [CrossRef][PubMed]
    [Google Scholar]
  22. Drexler JF, Corman VM, Drosten C. Ecology, evolution and classification of bat coronaviruses in the aftermath of SARS. Antiviral Res. 2014;10145–56 [CrossRef][PubMed]
  23. Hutterer R, Ivanova T, Meyer-Cord C, Rodrigues L. Bat migrations in Europe. Naturschutz Biol Vielfalt 2005;28:162
    [Google Scholar]
  24. Hutson AM, Aulagnier S, Benda P, Karataş A, Palmeirim J et al. 2008; Miniopterus schreibersii. In The IUCN Red List of Threatened Species IUCN, p.e.T13561A4160556http://iucnredlist.org/details/13561/0
  25. Streicker DG, Winternitz JC, Satterfield DA, Condori-Condori RE, Broos A et al. Host–pathogen evolutionary signatures reveal dynamics and future invasions of vampire bat rabies. Proc Natl Acad Sci USA 2016;113:10926–10931 [CrossRef][PubMed]
    [Google Scholar]
  26. Zuker M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 2003;31:3406–3415 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000760
Loading
/content/journal/jgv/10.1099/jgv.0.000760
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error