1887

Abstract

The Bunyaviridae family comprises viruses causing diseases of public and veterinary health importance, including viral haemorrhagic and arboviral fevers. We report the isolation, identification and genome characterization of a novel orthobunyavirus, named Wolkberg virus (WBV), from wingless bat fly ectoparasites (Eucampsipoda africana) of Egyptian fruit bats (Rousettus aegyptiacus) in South Africa. Complete genome sequence data of WBV suggests it is most closely related to two bat viruses (Mojuí dos Campos and Kaeng Khoi viruses) and an arbovirus (Nyando virus) previously shown to infect humans. WBV replicates to high titres in VeroE6 and C6-36 cells, characteristic of mosquito-borne arboviruses. These findings expand our knowledge of the diversity of orthobunyaviruses and their insect vector host range.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000753
2017-05-11
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/98/5/935.html?itemId=/content/journal/jgv/10.1099/jgv.0.000753&mimeType=html&fmt=ahah

References

  1. Elliott RM. Emerging viruses: the Bunyaviridae. Mol Med 1997;3:572–577[PubMed]
    [Google Scholar]
  2. Plyusnin A, Beaty BJ, Elliot RM, Goldbach R, Kormelink R et al. Family Bunyaviridae. In King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ. (editors) Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses San Diego, CA: Elsevier; 2012; pp.725–741
    [Google Scholar]
  3. Digoutte JP, Gagnard VJ, Bres P, Pajot FX. Nyando virus infection in man. Bull Soc Pathol Exot Filiales 1972;65:751–758[PubMed]
    [Google Scholar]
  4. Kalunda M, Lwanga-Ssozi C, Lule M, Mukuye A. Isolation of Chikungunya and Pongola viruses from patients in Uganda. Trans R Soc Trop Med Hyg 1985;79:567 [CrossRef][PubMed]
    [Google Scholar]
  5. Gonzalez JP, Georges AJ. Bunyaviral fevers: Bunyamwera, Ilesha, Germiston, Bwamba and Tataguine. In Monath T. (editor) The Arboviruses: Epidemiology and Ecology Boca Raton, FL: CRC Press; 1988; pp.87–98
    [Google Scholar]
  6. Lutwama JJ, Rwaguma EB, Nawanga PL, Mukuye A. Isolations of Bwamba virus from south central Uganda and north eastern Tanzania. Afr Health Sci 2002;2:24–28[PubMed]
    [Google Scholar]
  7. Gerrard SR, Li L, Barrett AD, Nichol ST. Ngari virus is a Bunyamwera virus reassortant that can be associated with large outbreaks of hemorrhagic fever in Africa. J Virol 2004;78:8922–8926 [CrossRef][PubMed]
    [Google Scholar]
  8. Briese T, Bird B, Kapoor V, Nichol ST, Lipkin WI. Batai and Ngari viruses: M segment reassortment and association with severe febrile disease outbreaks in East Africa. J Virol 2006;80:5627–5630 [CrossRef][PubMed]
    [Google Scholar]
  9. Bastos MS, Figueiredo LT, Naveca FG, Monte RL, Lessa N et al. Identification of oropouche orthobunyavirus in the cerebrospinal fluid of three patients in the Amazonas, Brazil. Am J Trop Med Hyg 2012;86:732–735 [CrossRef][PubMed]
    [Google Scholar]
  10. Byrd BD. La Crosse encephalitis: a persistent arboviral threat in North Carolina. N C Med J 2016;77:330–333 [CrossRef][PubMed]
    [Google Scholar]
  11. Pastula DM, Smith DE, Beckham JD, Tyler KL. Four emerging arboviral diseases in North America: Jamestown Canyon, Powassan, Chikungunya, and Zika virus diseases. J Neurovirol 2016;22:257–260 [CrossRef][PubMed]
    [Google Scholar]
  12. Charles JA. Akabane virus. Vet Clin North Am Food Anim Pract 1994;10:525–546 [CrossRef][PubMed]
    [Google Scholar]
  13. van Eeden C, Williams JH, Gerdes TG, van Wilpe E, Viljoen A et al. Shuni virus as cause of neurologic disease in horses. Emerg Infect Dis 2012;18:318–321 [CrossRef][PubMed]
    [Google Scholar]
  14. Beer M, Conraths FJ, van der Poel WH. 'Schmallenberg virus: a novel orthobunyavirus emerging in Europe. Epidemiol Infect 2013;141:1–8 [CrossRef][PubMed]
    [Google Scholar]
  15. Williams JE, Imlarp S, Top FH, Cavanaugh DC, Russell PK. Kaeng Khoi virus from naturally infected bedbugs (Cimicidae) and immature free-tailed bats. Bull World Health Organ 1976;53:365–369[PubMed]
    [Google Scholar]
  16. Wanzeller AL, Diniz JA, Gomes ML, Cruz AC, Soares MC et al. Ultrastructural, antigenic and physicochemical characterization of the Mojuí dos Campos (Bunyavirus) isolated from bat in the Brazilian Amazon region. Mem Inst Oswaldo Cruz 2002;97:307–311 [CrossRef][PubMed]
    [Google Scholar]
  17. Groseth A, Mampilli V, Weisend C, Dahlstrom E, Porcella SF et al. Molecular characterization of human pathogenic bunyaviruses of the Nyando and Bwamba/Pongola virus groups leads to the genetic identification of Mojuí dos Campos and Kaeng Khoi virus. PLoS Negl Trop Dis 2014;8:e3147 [CrossRef][PubMed]
    [Google Scholar]
  18. Osborne JC, Rupprecht CE, Olson JG, Ksiazek TG, Rollin PE et al. Isolation of Kaeng Khoi virus from dead Chaerephon plicata bats in Cambodia. J Gen Virol 2003;84:2685–2689 [CrossRef][PubMed]
    [Google Scholar]
  19. Theodor O. An Illustrated Catalogue of the Rothschild Collection of Nycteribiidae (Diptera) in the British Museum (Natural History), with Keys and Short Descriptions for the Identification of Subfamilies, Genera, Species and Subspecies London, UK: British Museum (Natural History); 1967
    [Google Scholar]
  20. Jansen van Vuren P, Wiley M, Palacios G, Storm N, Mcculloch S et al. Isolation of a novel fusogenic Orthoreovirus from Eucampsipoda africana bat flies in South Africa. Viruses 2016;8:65 [CrossRef]
    [Google Scholar]
  21. Bowden TA, Bitto D, Mclees A, Yeromonahos C, Elliott RM et al. Orthobunyavirus ultrastructure and the curious tripodal glycoprotein spike. PLoS Pathog 2013;9:e1003374 [CrossRef]
    [Google Scholar]
  22. Albornoz A, Hoffmann A, Lozach P-Y, Tischler N. Early Bunyavirus-host cell interactions. Viruses 2016;8:143 [CrossRef]
    [Google Scholar]
  23. Fontana J, López-Montero N, Elliott RM, Fernández JJ, Risco C. The unique architecture of Bunyamwera virus factories around the Golgi complex. Cell Microbiol 2008;10:2012–2028 [CrossRef]
    [Google Scholar]
  24. Walter CT, Barr JN. Recent advances in the molecular and cellular biology of bunyaviruses. J Gen Virol 2011;92:2467–2484 [CrossRef][PubMed]
    [Google Scholar]
  25. Goldsmith C. Morphologic differentiation of viruses beyond the family level. Viruses 2014;6:4902–4913 [CrossRef]
    [Google Scholar]
  26. Elliott RM. Nucleotide sequence analysis of the large (L) genomic RNA segment of Bunyamwera virus, the prototype of the family Bunyaviridae. Virology 1989;173:426–436 [CrossRef]
    [Google Scholar]
  27. Fazakerley J, Gonzalezscarano F, Strickler J, Dietzschold B, Karush F et al. Organization of the middle RNA segment of snowshoe hare bunyavirus. Virology 1988;167:422–432 [CrossRef]
    [Google Scholar]
  28. Savji N, Palacios G, Travassos da Rosa A, Hutchison S, Celone C et al. Genomic and phylogenetic characterization of leanyer virus, a novel orthobunyavirus isolated in northern Australia. J Gen Virol 2011;92:1676–1687 [CrossRef][PubMed]
    [Google Scholar]
  29. Eifan SA, Elliott RM. Mutational analysis of the Bunyamwera orthobunyavirus nucleocapsid protein gene. J Virol 2009;83:11307–11317 [CrossRef]
    [Google Scholar]
  30. Bridgen A, Weber F, Fazakerley JK, Elliott RM. Bunyamwera bunyavirus nonstructural protein NSs is a nonessential gene product that contributes to viral pathogenesis. Proc Natl Acad Sci USA 2001;98:664–669 [CrossRef]
    [Google Scholar]
  31. Weber F, Bridgen A, Fazakerley JK, Streitenfeld H, Kessler N et al. Bunyamwera bunyavirus nonstructural protein NSs counteracts the induction of alpha/beta interferon. J Virol 2002;76:7949–7955 [CrossRef][PubMed]
    [Google Scholar]
  32. Kohl A, Clayton RF, Weber F, Bridgen A, Randall RE et al. Bunyamwera virus nonstructural protein NSs counteracts interferon regulatory factor 3-mediated induction of early cell death. J Virol 2003;77:7999–8008 [CrossRef]
    [Google Scholar]
  33. Szemiel AM, Failloux A-B, Elliott RM. Role of Bunyamwera orthobunyavirus NSs protein in infection of mosquito cells. PLoS Negl Trop Dis 2012;6:e1823 [CrossRef]
    [Google Scholar]
  34. Mohamed M, Mclees A, Elliott RM. Viruses in the Anopheles A, Anopheles B, and Tete serogroups in the Orthobunyavirus genus (family Bunyaviridae) do not encode an NSs protein. J Virol 2009;83:7612–7618 [CrossRef][PubMed]
    [Google Scholar]
  35. Hart TJ, Kohl A, Elliott RM. Role of the NSs protein in the zoonotic capacity of orthobunyaviruses. Zoonoses Public Health 2008;56:285–296 [CrossRef]
    [Google Scholar]
  36. Causey OR, Causey CE, Maroja OM, Macedo DG. The isolation of arthropod-borne viruses, including members of two hitherto undescribed serological groups, in the Amazon region of Brazil. Am J Trop Med Hyg 1961;10:227–249[CrossRef]
    [Google Scholar]
  37. Iversson LB, Silva RA, da Rosa AP, Barros VL. Circulation of eastern equine encephalitis, western equine encephalitis, Ilhéus, Maguari and Tacaiuma viruses in equines of the Brazilian Pantanal, South America. Rev Inst Med Trop Sao Paulo 1993;35:355–359 [CrossRef][PubMed]
    [Google Scholar]
  38. Morlan JD, Qu K, Sinicropi DV. Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue. PLoS One 2012;7:e42882 [CrossRef]
    [Google Scholar]
  39. Martin M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J 2011;17:10 [CrossRef]
    [Google Scholar]
  40. Schmieder R, Edwards R. Quality control and preprocessing of metagenomic datasets. Bioinformatics 2011;27:863–864 [CrossRef]
    [Google Scholar]
  41. Boisvert S, Raymond F, Godzaridis Élénie, Laviolette F, Corbeil J. Ray Meta: scalable de novo metagenome assembly and profiling. Genome Biol 2012;13:R122 [CrossRef]
    [Google Scholar]
  42. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef]
    [Google Scholar]
  43. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993;1993:512–526
    [Google Scholar]
  44. Ladner JT, Beitzel B, Chain PSG, Davenport MG, Donaldson E et al. Standards for sequencing viral genomes in the era of high-throughput sequencing. MBio 2014;5:e01360-14 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000753
Loading
/content/journal/jgv/10.1099/jgv.0.000753
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error