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Volume 97, Issue 9

Functional identification of two minor capsid proteins from Chinese wheat mosaic virus using its infectious full-length cDNA clones Free

Abstract

Full-length cDNA clones of Chinese wheat mosaic virus (CWMV) RNA1 and RNA2 were produced from single reverse transcription PCR reactions and transcripts were shown to be infectious in both wheat and . An efficient and reliable agro-infiltration method was then developed for reverse genetic assays in . Inoculation of infectious cDNA clones resulted in obvious chlorotic symptoms, and CWMV viral genomic RNAs, capsid protein (CP)-related proteins, and typical rod-shaped particles were detectable on the inoculated and upper leaves, similar to those of WT virus. The optimal temperature for virus multiplication was 12 °C, but the optimum for systematic infection in plants was 17 °C. Mutant clones that abolished the N- or C-terminal extensions of the major CP did not inhibit systemic infection or the formation of rod-shaped particles but sometimes modified the symptoms in inoculated plants. These results suggest that the two minor CP-related proteins of CWMV are dispensable for viral infection, replication, systemic movement and virion assembly in plants.

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Keyword(s): capsid protein (CP) , Chinese wheat mosaic furovirus (CWMV) , infectious cDNA clones and minor capsid proteins
© 2016 The Authors
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2016-09-01
2024-05-07
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Functional identification of two minor capsid proteins from Chinese wheat mosaic virus using its infectious full-length cDNA clones
J. Gen. Virol. 97, 2441 (2016); https://doi.org/10.1099/jgv.0.000532
/content/journal/jgv/10.1099/jgv.0.000532
/content/journal/jgv/10.1099/jgv.0.000532
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