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Journal of General Virology header logo Journal of General Virology

Volume 97, Issue 8

The putative U94 integrase is dispensable for human herpesvirus 6 (HHV-6) chromosomal integration No Access

Abstract

Human herpesvirus 6 (HHV-6) can integrate its genome into the telomeres of host chromosomes and is present in the germline of about 1 % of the human population. HHV-6 encodes a putative integrase U94 that possesses all molecular functions required for recombination including DNA-binding, ATPase, helicase and nuclease activity, and was hypothesized by many researchers to facilitate integration ever since the discovery of HHV-6 integration. However, analysis of U94 in the virus context has been hampered by the lack of reverse-genetic systems and efficient integration assays. Here, we addressed the role of U94 and the cellular recombinase Rad51 in HHV-6 integration. Surprisingly, we could demonstrate that HHV-6 efficiently integrated in the absence of U94 using a new quantitative integration assay. Additional inhibition of the cellular recombinase Rad51 had only a minor impact on virus integration. Our results shed light on this complex integration mechanism that includes factors beyond U94 and Rad51.

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Keyword(s): Human herpesvirus 6 , integrase , integration , latency , telomeres and U94
© 2016 The Authors
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/content/journal/jgv/10.1099/jgv.0.000502
2016-08-01
2026-02-17

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/content/journal/jgv/10.1099/jgv.0.000502
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The putative U94 integrase is dispensable for human herpesvirus 6 (HHV-6) chromosomal integration
J. Gen. Virol. 97, 1899 (2016); https://doi.org/10.1099/jgv.0.000502
/content/journal/jgv/10.1099/jgv.0.000502
/content/journal/jgv/10.1099/jgv.0.000502
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