1887

Abstract

Bats are important reservoirs of many viruses with zoonotic potential worldwide, including Europe. Among bat viruses, members of the family remain a neglected group. We performed viral metagenomic analyses on bat faecal samples, collected in Hungary in 2013. In the present study we report the first molecular data and genomic characterization of a novel picornavirus from the bat species in Europe. Based on phylogenetic analyses, the novel bat picornaviruses unambiguously belong to the genus and were highly divergent from other bat-derived picornaviruses of the genus. Although the Hungarian viruses were most closely related to Mischivirus A, they formed a separate monophyletic branch within the genus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000028
2015-04-01
2020-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/4/815.html?itemId=/content/journal/jgv/10.1099/jgv.0.000028&mimeType=html&fmt=ahah

References

  1. Adams M. J., King A. M. Q., Carstens E. B. 2013; Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses (2013). Arch Virol 158:2023–2030 [CrossRef][PubMed]
    [Google Scholar]
  2. Appleton B. R., McKenzie J. A., Christidis L. 2004; Molecular systematics and biogeography of the bent-wing bat complex Miniopterus schreibersii (Kuhl, 1817) (Chiroptera: Vespertilionidae). Mol Phylogenet Evol 31:431–439 [CrossRef][PubMed]
    [Google Scholar]
  3. Buckwalter M. R., Nga P. T., Gouilh M. A., Fiette L., Bureau J. F., Laird M. E., Buchrieser J., Ozden S., Cheval J.other authors 2011; Identification of a novel neuropathogenic Theiler’s murine encephalomyelitis virus. J Virol 85:6893–6905 [CrossRef][PubMed]
    [Google Scholar]
  4. Chen H. H., Kong W. P., Roos R. P. 1995; The leader peptide of Theiler’s murine encephalomyelitis virus is a zinc-binding protein. J Virol 69:8076–8078[PubMed]
    [Google Scholar]
  5. Duke G. M., Hoffman M. A., Palmenberg A. C. 1992; Sequence and structural elements that contribute to efficient encephalomyocarditis virus RNA translation. J Virol 66:1602–1609[PubMed]
    [Google Scholar]
  6. Duque H., Palmenberg A. C. 2001; Phenotypic characterization of three phylogenetically conserved stem-loop motifs in the mengovirus 3′ untranslated region. J Virol 75:3111–3120 [CrossRef][PubMed]
    [Google Scholar]
  7. Fehér E., Pazár P., Kovács E., Farkas S. L., Lengyel G., Jakab F., Martella V., Bányai K. 2014; Molecular detection and characterization of human gyroviruses identified in the ferret fecal virome. Arch Virol 159:3401–3406 [CrossRef][PubMed]
    [Google Scholar]
  8. Gorbalenya A. E., Koonin E. V., Lai M. M. 1991; Putative papain-related thiol proteases of positive-strand RNA viruses. Identification of rubi- and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubi-, alpha- and coronaviruses. FEBS Lett 288:201–205 [CrossRef][PubMed]
    [Google Scholar]
  9. Kapoor A., Victoria J., Simmonds P., Slikas E., Chieochansin T., Naeem A., Shaukat S., Sharif S., Alam M. M.other authors 2008; A highly prevalent and genetically diversified Picornaviridae genus in South Asian children. Proc Natl Acad Sci U S A 105:20482–20487 [CrossRef][PubMed]
    [Google Scholar]
  10. Knowles N. J., Hovi T., Hyypiä T., King A. M. Q., Lindberg M., Pallansch M. A., Palmenberg A. C., Simmonds P., Skern T.other authors 2012; Picornaviridae. In Virus Taxonomy: Classification and Nomenclature of Viruses: Ninth Report of the International Committee on Taxonomy of Viruses pp. 855–880 Edited by King A. M. Q., Adams M. J., Carstens E. B., Lefkowitz E. J. San Diego: Elsevier;
    [Google Scholar]
  11. Kohl C., Kurth A. 2014; European bats as carriers of viruses with zoonotic potential. Viruses 6:3110–3128 [CrossRef][PubMed]
    [Google Scholar]
  12. Lau S. K., Woo P. C., Lai K. K., Huang Y., Yip C. C., Shek C. T., Lee P., Lam C. S., Chan K. H., Yuen K. Y. 2011; Complete genome analysis of three novel picornaviruses from diverse bat species. J Virol 85:8819–8828 [CrossRef][PubMed]
    [Google Scholar]
  13. Law K. M., Brown T. D. 1990; The complete nucleotide sequence of the GDVII strain of Theiler’s murine encephalomyelitis virus (TMEV). Nucleic Acids Res 18:6707–6708 [CrossRef][PubMed]
    [Google Scholar]
  14. Liang Z., Kumar A. S., Jones M. S., Knowles N. J., Lipton H. L. 2008; Phylogenetic analysis of the species Theilovirus: emerging murine and human pathogens. J Virol 82:11545–11554 [CrossRef][PubMed]
    [Google Scholar]
  15. Lim E. S., Cao S., Holtz L. R., Antonio M., Stine O. C., Wang D. 2014; Discovery of rosavirus 2, a novel variant of a rodent-associated picornavirus, in children from The Gambia. Virology 454-455:25–33 [CrossRef][PubMed]
    [Google Scholar]
  16. Marchler-Bauer A., Lu S., Anderson J. B., Chitsaz F., Derbyshire M. K., DeWeese-Scott C., Fong J. H., Geer L. Y., Geer R. C.other authors 2011; CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Res 39:Database issueD225–D229 [CrossRef][PubMed]
    [Google Scholar]
  17. Myoung J., Hou W., Kang B., Lyman M. A., Kang J. A., Kim B. S. 2007; The immunodominant CD8+ T cell epitope region of Theiler’s virus in resistant C57BL/6 mice is critical for anti-viral immune responses, viral persistence, and binding to the host cells. Virology 360:159–171 [CrossRef][PubMed]
    [Google Scholar]
  18. Pevear D. C., Calenoff M., Rozhon E., Lipton H. L. 1987; Analysis of the complete nucleotide sequence of the picornavirus Theiler’s murine encephalomyelitis virus indicates that it is closely related to cardioviruses. J Virol 61:1507–1516[PubMed]
    [Google Scholar]
  19. Rohll J. B., Moon D. H., Evans D. J., Almond J. W. 1995; The 3′ untranslated region of picornavirus RNA: features required for efficient genome replication. J Virol 69:7835–7844[PubMed]
    [Google Scholar]
  20. Sikes R. S., Gannon W. L. 2011; Guidelines of the American Society of Mammalogists for the use of wild mammals in research. J Mammal 92:235–253 [CrossRef]
    [Google Scholar]
  21. Sorgeloos F., Jha B. K., Silverman R. H., Michiels T. 2013; Evasion of antiviral innate immunity by Theiler’s virus L* protein through direct inhibition of RNase L. PLoS Pathog 9:e1003474 [CrossRef][PubMed]
    [Google Scholar]
  22. Teeling E. C., Springer M. S., Madsen O., Bates P., O’brien S. J., Murphy W. J. 2005; A molecular phylogeny for bats illuminates biogeography and the fossil record. Science 307:580–584 [CrossRef][PubMed]
    [Google Scholar]
  23. Tracy S., Chapman N. M., Drescher K. M., Kono K., Tapprich W. 2006; Evolution of virulence in picornaviruses. Curr Top Microbiol Immunol 299:193–209[PubMed]
    [Google Scholar]
  24. Uhrin M., Boldogh S., Bücs S., Paunovic M., Miková E., Juhász M., Csősz I., Estók P., Fulin M.other authors 2012; Revision of the occurrence of Rhinolophus euryale in the Carpathian region, Central Europe. Vespertilio 16:289–328
    [Google Scholar]
  25. Wu Z., Ren X., Yang L., Hu Y., Yang J., He G., Zhang J., Dong J., Sun L.other authors 2012; Virome analysis for identification of novel mammalian viruses in bat species from Chinese provinces. J Virol 86:10999–11012 [CrossRef][PubMed]
    [Google Scholar]
  26. Yu J. M., Li X. Y., Ao Y. Y., Li L. L., Liu N., Li J. S., Duan Z. J. 2013; Identification of a novel picornavirus in healthy piglets and seroepidemiological evidence of its presence in humans. PLoS ONE 8:e70137 [CrossRef][PubMed]
    [Google Scholar]
  27. Zuker M. 2003; Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000028
Loading
/content/journal/jgv/10.1099/jgv.0.000028
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error