1887

Abstract

SUMMARY

Production of Sendai virus and virus-induced ultrastructural changes were compared in infected chick embryo lung epithelial cells and chick embryo fibroblast cells. By 48 hr after infection, chick embryo lung cells made 100 times more infectious virus, as measured by plaque assay, than chick embryo fibroblast cells. The chick embryo lung cells showed little cytopathology up to 48 hr, while chick embryo fibroblast cells began to show severe cytopathic changes at 24 hr. Both cell types contained abundant cytoplasmic aggregates of virus nucleocapsids, but budding virus particles were more common on the surfaces of chick embryolung cells. The data indicate that late steps in virus maturation are relatively inefficient in chick embryo fibroblast cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-9-3-169
1970-12-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/9/3/JV0090030169.html?itemId=/content/journal/jgv/10.1099/0022-1317-9-3-169&mimeType=html&fmt=ahah

References

  1. Barry R. D. 1965; Factors which affect the release of Newcastle disease and Sendai viruses from infected allantoic cells. Journal of General Microbiology 39:229
    [Google Scholar]
  2. Barry R. D., Bukrinskaya A. G. 1968; The nucleic acid of Sendai virus and ribonucleic acid synthesis in cells infected by Sendai virus. Journal of General Virology 2:71
    [Google Scholar]
  3. Blair C. D., Robinson W. S. 1968; Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology 35:537
    [Google Scholar]
  4. Bukrinskaya A. G., Zhdanov V. M. 1963; Shortening by actinomycin D of latent period of multiplication of Sendai virus. Nature, London 200:920
    [Google Scholar]
  5. Chanock R. M., Parrott R. H. 1965; Para-influenza viruses. In Viral and Rickettsial Infections of Man p 741 Ed. by Horsfall F. L. Jun., Tamm I. Philadelphia: J. B. Lippincott;
    [Google Scholar]
  6. Compans R. W., Holmes K. V., Dales S., Choppin P. W. 1964; An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV 5. Virology 30:411
    [Google Scholar]
  7. Darlington R. W., Portner A., Kingsbury D. W. 1969; Comparison of the growth of Sendai and New castle disease virus in chick embryo fibroblast and lung cells. Bacteriological Proceedings 176:
    [Google Scholar]
  8. Franklin R. M., Rubin H., Davis C. A. 1957; The production, purification, and properties of Newcastle disease virus labeled with radiophosphorus. Virology 3:96
    [Google Scholar]
  9. Gresser . 1961; Induction by Sendai virus of non-transmissible cytopathic changes associated with rapid and marked production of interferon. Proceedings of the Society for Experimental Biology and Medicine 108:303
    [Google Scholar]
  10. Gresser I., Enders J. F. 1961; The effect of trypsin on representative myxoviruses. Virology 13:420
    [Google Scholar]
  11. Heath R. B., Tyrrell D. A. J. 1958; The behavior of some influenza viruses in tissue cultures of kidney cells of various species. Archiv fiir die gesamte Virusforschung 8:577
    [Google Scholar]
  12. Holmes K. V., Choppin P. W. 1966; On the role of the response of the cell membrane in determining virus virulence. Contrasting effects of the parainfluenza virus SV 5 in two cell types. Journal of Experimental Medicine 124:501
    [Google Scholar]
  13. Jensen K. E., Minuse E., Ackermann W. W. 1955; Serologic evidence of American experience with new born pneumonitis virus (type Sendai). Journal of Immunology 75:71
    [Google Scholar]
  14. Kingsbury D. W., Darlington R. W. 1968; Isolation and properties of Newcastle disease virus nucleo-capsid. Journal of Virology 2:248
    [Google Scholar]
  15. Kingsbury D. W., Portner A. 1970; On the genesis of incomplete Sendai virions. Virology (In the Press)
    [Google Scholar]
  16. Kingsbury D. W., Portner A., Darlington R. W. 1970; Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology (In the Press)
    [Google Scholar]
  17. Mannweiler E. 1960; Das Verhalten von Influenzaviren in Gewebekulturen von Huhnerembryo-Nieren. II. Virusmehrung. Archiv fiir die gesamte Virusforschung 10:647
    [Google Scholar]
  18. Matsumoto T., Maeno K. 1961; Growth of HVJ (hemagglutinating virus of Japan) in mouse lung cells. Japanese Journal of Microbiology 5:89
    [Google Scholar]
  19. Northrop R. L., Walker D. L. 1965; A stable human cell line as a host system for Sendai virus. Proceedings of the Society for Experimental Biology and Medicine 118:698
    [Google Scholar]
  20. Okada Y., Tadokoro J. 1963; The distribution of cell fusion capacity among several cell strains or cells caused by HVJ. Experimental Cell Research 32:417
    [Google Scholar]
  21. Portner A., Kingsbury D. W. 1969; Sendai virus RNA synthesis in chick embryo lung epithelial cells. Bacteriological Proceedings176
    [Google Scholar]
  22. Reed L. J., Muench H. 1938; A simple method of estimating 50% endpoints. American Journal of Hygiene 27:493
    [Google Scholar]
  23. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17:208
    [Google Scholar]
  24. Shimizu T., Ishizaki R., Kono Y., Kumagai T., Arai S., Sasahara J., Ishii S., Matumoto M. 1955; Multiplication and cytopathogenic effect of ‘the hemagglutinating virus of Japan’ (HVJ) in swine kidney tissue culture. Japanese Journal of Experimental Medicine 25:211
    [Google Scholar]
  25. Stempak J. G., Ward R. T. 1964; An improved staining method for electron microscopy. Journal of Cell Biology 22:697
    [Google Scholar]
  26. Waterson A. P. 1962; Two kinds of myxoviruses. Nature, London 193:1163
    [Google Scholar]
  27. Zhdanov V. M., Bukrinskaya A. G., Azadova N. B. 1961; Fluorescent microscopic study of Sendai incomplete virus production in tissue culture cells. Journal of Immunology 87:647
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-9-3-169
Loading
/content/journal/jgv/10.1099/0022-1317-9-3-169
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error