1887

Abstract

Studies on the three major adenovirus structural antigens, designated (a base plus a fibre) and fibres (Ginsberg 1966), have been reviewed extensively (Norrby, 1968; Schlesinger, 1969). The availability of purified preparations of hexon, fibre and whole penton has made the physicochemical and immunological characterization of these virus components possible. However, the properties of the penton base, a component also known to be synthesized independently in infected cells, have received relatively little attention, mainly because of the lack of a method for its purification. Present information on the properties of the penton base is derived from analytical data on partially purified materials or from the study of whole penton (Pettersson & Hoglund, 1969; Wadell & Norrby, 1969). In the present report we describe a simple method for the preparation of physicochemically and immunologically pure adenovirus type 5 (ad. 5) penton base.

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1970-10-01
2021-10-17
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References

  1. Brownstone A. D. 1969; A versatile system for preparative electrophoresis in acrylamide gel. Analytical Biochemistry 27:24
    [Google Scholar]
  2. Ginsburg H. S., Pereira H. G., Valentine R. C., Wilcox W. C. 1966; A proposed terminology for the adenovirus antigens and virion morphological subunits. Virology 28:782
    [Google Scholar]
  3. Maizel J. V., White D. O., Scharff M. D. 1968; The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7a and 12. Virology 36:115
    [Google Scholar]
  4. Norby E. 1968; Biological significance of structural adenovirus components. Current Topics in Microbiology and Immunology Berlin: Springer Verlag; 431
    [Google Scholar]
  5. Norby E. 1969; The structural and functional diversity of adenovirus capsid components. Journal of General Virology 5:221
    [Google Scholar]
  6. Pereira H. G. 1958; A protein factor responsible for early cytopathic effect of adenoviruses. Virology 6:601
    [Google Scholar]
  7. Pereira H. G., De Figueiredo M. V. T. 1962; Mechanism of haemagglutination by adenovirus types 1, 2, 4, 5 and 6. Virology 18:1
    [Google Scholar]
  8. Pereira H. G., Valentine R. C., Russell W. C. 1968; Crystallization of an adenovirus protein (the hexon). Nature London 219:946
    [Google Scholar]
  9. Pettersson V., Hoglund S. 1969; Structural proteins of adenoviruses. III. Purification and characterization of the adenovirus type 2 penton antigen. Virology 39:90
    [Google Scholar]
  10. Russell W. C., Hayashi K., Sanderson P. J., Pereira H. G. 1967a; Adenovirus antigens–a study of their properties and sequential development in infection. Journal of General Virology 1:495
    [Google Scholar]
  11. Russell W. C., Valentine R. C., Pereira H. G. 1967b; The effect of heat on the anatomy of the adenovirus. Journal of General Virology 1:509
    [Google Scholar]
  12. Schlesinger R. W. 1969; Adenoviruses: The nature of the virion and of controlling factors in productive or abortive infection and tumorigenesis. Advances in Virus Research 14:1
    [Google Scholar]
  13. Valentine R. C., Pereira H. G. 1965; Antigens and structure of the adenovirus. Journal of Molecular Biology 13:13
    [Google Scholar]
  14. Wadell G., Norrby E. 1969; Immunological and other biological characteristics of pentons of human adenoviruses. Journal of Virology 4:671
    [Google Scholar]
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