1887

Abstract

Late gene expression factors, LEF-4, LEF-8, LEF-9 and P47 constitute the primary components of the multinucleocapsid polyhedrovirus (AcMNPV)-encoded RNA polymerase, which initiates transcription from late and very late promoters. Here, characterization of -9 and -8, which encode their corresponding counterparts, from NPV is reported. Transcription of -9 initiated at two independent sites: from a CCT sequence located at −38 nt and a TCTT sequence located at −50 nt, with respect to the +1 ATG of the open reading frame. The 3′ end of the transcript was mapped to a site 17 nt downstream of a canonical polyadenylation signal located 7 nt downstream of the first of the two tandem translational termination codons. Maximum synthesis of LEF-9 was seen from 36 h post-infection (p.i.). The transcription of -8 initiated early in infection from a TGCAAT sequence that differed in the corresponding region from its AcMNPV counterpart (GCG), with consequent elimination of the consensus early transcription start site motif (underlined). Peak levels of -8 transcripts were attained by 24 h p.i. Immunocopurification analyses suggested that there was an association between LEF-8 and LEF-9 .

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2002-08-01
2020-10-20
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