1887

Abstract

Scrapie does not occur in New Zealand (NZ), although PrP gene alleles associated with susceptibility to the disease are found at relatively high frequencies in NZ sheep. The hypothesis that scrapie is a genetic disease of sheep is thus unlikely to be true. To confirm that NZ sheep are actually susceptible to scrapie infection, NZ sheep of various PrP genotypes were challenged by subcutaneous inoculation with a sheep-passaged scrapie isolate (SSBP/1). Showing similar PrP genetics to that seen in UK sheep, all NZ sheep carrying the VRQ PrP allele developed clinical signs typical of scrapie, with characteristic neurodegenerative changes and PrP evident on histopathological examination of their brains and lymphoid tissues. The incubation periods recorded in NZ sheep were generally shorter than those found in UK sheep. The results confirm that New Zealand sheep are as susceptible as their UK counterparts to experimental scrapie infection by subcutaneous inoculation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-83-5-1247
2002-05-01
2019-12-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/83/5/0831247a.html?itemId=/content/journal/jgv/10.1099/0022-1317-83-5-1247&mimeType=html&fmt=ahah

References

  1. Andréoletti, O., Berthon, P., Marc, D., Sarradin, P., Grosclaude, J., van Keulen, L., Schelcher, F., Elsen, J. M. & Lantier, F. ( 2000; ). Early accumulation of PrPSc in gut-associated lymphoid and nervous tissues of susceptible sheep from a Romanov flock with natural scrapie. Journal of General Virology 81, 3115-3126.
    [Google Scholar]
  2. Belt, P. B. G. M., Muileman, I. H., Schreuder, B. E. C., Bos-de Ruijter, J., Gielkens, A. L. J. & Smits, M. A. ( 1995; ). Identification of five allelic variants of the sheep PrP gene and their association with natural scrapie. Journal of General Virology 76, 509-517.[CrossRef]
    [Google Scholar]
  3. Bossers, A., Harders, F. L. & Smits, M. A. ( 1999; ). PrP genotype frequencies of the most dominant sheep breed in a country free from scrapie. Archives of Virology 144, 829-834.[CrossRef]
    [Google Scholar]
  4. Dickinson, A. G., Stamp, J. T., Renwick, C. C. & Rennie, J. C. ( 1968; ). Some factors controlling the incidence of scrapie in Cheviot sheep injected with a Cheviot-passaged scrapie agent. Journal of Comparative Pathology 78, 313-321.[CrossRef]
    [Google Scholar]
  5. Foster, J. D., Wilson, M. & Hunter, N. ( 1996; ). Immunolocalisation of the prion protein (PrP) in the brains of sheep with scrapie. Veterinary Record. 139, 512-515.[CrossRef]
    [Google Scholar]
  6. Goldmann, W., Hunter, N., Benson, G., Foster, G. D. & Hope, J. ( 1991; ). Different scrapie-associated fibril proteins (PrP) are encoded by lines of sheep selected for different alleles of the Sip gene. Journal of General Virology 72, 2411-2417.[CrossRef]
    [Google Scholar]
  7. Goldmann, W., Hunter, N., Smith, G., Foster, J. & Hope, J. ( 1994; ). PrP genotype and agent effects in scrapie: change in allelic interaction with different isolates of agent in sheep, a natural host of scrapie. Journal of General Virology 75, 989-995.[CrossRef]
    [Google Scholar]
  8. Haritani, M., Spencer, Y. I. & Wells, G. A. H. ( 1994; ). Hydrated autoclave pretreatment enhancement of prion protein immunoreactivity in formalin-fixed bovine spongiform encephalopathy-affected brain. Acta Neuropathologica 87, 86-90.[CrossRef]
    [Google Scholar]
  9. Hourrigan, J., Klingsporn, A., Clark, W. W. & de Camp, M. ( 1979; ). Epidemiology of scrapie in the United States. In Slow Transmissible Diseases of the Nervous System , pp. 331-356. Edited by S. Prusiner & W. J. Hadlow. New York:Academic Press.
  10. Hunter, N. ( 1997; ). PrP genetics in sheep and the implications for scrapie and BSE. Trends in Microbiology 5, 331-334.[CrossRef]
    [Google Scholar]
  11. Hunter, N. & Cairns, D. ( 1998; ). Scrapie-free Merino and Poll Dorset sheep from Australia and New Zealand have normal frequencies of scrapie-susceptible PrP genotypes. Journal of General Virology 79, 2079-2082.
    [Google Scholar]
  12. Hunter, N., Goldmann, W., Benson, G., Foster, J. D. & Hope, J. ( 1993; ). Swaledale sheep affected by natural scrapie differ significantly in PrP genotype frequencies from healthy sheep and those selected for reduced incidence of scrapie. Journal of General Virology 74, 1025-1031.[CrossRef]
    [Google Scholar]
  13. Hunter, N., Foster, J. D., Goldmann, W., Stear, M. J., Hope, J. & Bostock, C. ( 1996; ). Natural scrapie in a closed flock of Cheviot sheep occurs only in specific PrP genotypes. Archives of Virology 141, 809-824.[CrossRef]
    [Google Scholar]
  14. Hunter, N., Moore, L., Hosie, B. D., Dingwall, W. S. & Greig, A. ( 1997a; ). Association between natural scrapie and PrP genotype in a flock of Suffolk sheep in Scotland. Veterinary Record 140, 59-63.[CrossRef]
    [Google Scholar]
  15. Hunter, N., Cairns, D., Foster, J. D., Smith, G., Goldmann, W. & Donnelly, K. ( 1997b; ). Is scrapie solely a genetic disease? Nature 386, 137.
    [Google Scholar]
  16. Ikeda, T., Horiuchi, M., Ishiguro, N., Muramatsu, Y., Kai-Uwe, G. D. & Shinagawa, M. ( 1995; ). Amino acid polymorphisms of PrP with reference to onset of scrapie in Suffolk and Corriedale sheep in Japan. Journal of General Virology 76, 2577-2581.[CrossRef]
    [Google Scholar]
  17. Ridley, R. M. & Baker, H. F. ( 1995; ). The myth of maternal transmission of spongiform encephalopathy. British Medical Journal 311, 1071-1075.[CrossRef]
    [Google Scholar]
  18. Ridley, R. M. & Baker, H. F. (1998). Fatal Protein. The Story of CJD, BSE and other Prion Diseases. Oxford: Oxford University Press.
  19. van Keulen, L. J. M., Schreuder, B. E. C., Meloen, R. H., Poelen van den berg, M., Mooij-Harkes, G., Vromans, M. E. W. & Langeveld, J. P. M. ( 1995; ). Immunohistochemical detection and localization of prion protein in brain tissue of sheep with natural scrapie. Veterinary Pathology 32, 299-308.[CrossRef]
    [Google Scholar]
  20. van Keulen, L. J. M., Screuder, B. E. C., Meloen, R. H., Mooij-Harkes, G., Vromans, M. E. W. & Langeveld, J. P. M. ( 1996; ). Immunohistochemical detection of prion protein in lymphoid tissues of sheep with natural scrapie. Journal of Clinical Microbiology 34, 1228-1231.
    [Google Scholar]
  21. Wilson, D. R., Anderson, R. D. & Smith, W. ( 1950; ). Studies in scrapie. Journal of Comparative Pathology 60, 267-282.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-83-5-1247
Loading
/content/journal/jgv/10.1099/0022-1317-83-5-1247
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error