Replacing the SCR domains of vaccinia virus protein B5R with EGFP causes a reduction in plaque size and actin tail formation but enveloped virions are still transported to the cell surface

References

1. Blasco R., Moss B. 1991; Extracellular vaccinia virus formation and cell-to-cell virus transmission are prevented by deletion of the gene encoding the 37,000-Dalton outer envelope protein. Journal of Virology 65:5910–5920
   [Google Scholar]
2. Cudmore S., Cossart P., Griffiths G., Way M. 1995; Actin-based motility of vaccinia virus. Nature 378:636–638
   [Google Scholar]
3. Cudmore S., Reckmann I., Griffiths G., Way M. 1996; Vaccinia virus: a model system for actin–membrane interactions. Journal of Cell Science 109:1739–1747
   [Google Scholar]
4. Engelstad M., Smith G. L. 1993; The vaccinia virus 42-kDa envelope protein is required for the envelopment and egress of extracellular virus and for virus virulence. Virology 194:627–637
   [Google Scholar]
5. Engelstad M., Howard S. T., Smith G. L. 1992; A constitutively expressed vaccinia gene encodes a 42-kDa glycoprotein related to complement control factors that forms part of the extracellular virus envelope. Virology 188:801–810
   [Google Scholar]
6. Falkner F. G., Moss B. 1990; Transient dominant selection of recombinant vaccinia viruses. Journal of Virology 64:3108–3111
   [Google Scholar]
7. Galmiche M. C., Goenaga J., Wittek R., Rindisbacher L. 1999; Neutralizing and protective antibodies directed against vaccinia virus envelope antigens. Virology 254:71–80
   [Google Scholar]
8. Herrera E., del Mar Lorenzo M., Blasco R., Isaacs S. N. 1998; Functional analysis of vaccinia virus B5R protein: essential role in virus envelopment is independent of a large portion of the extracellular domain. Journal of Virology 72:294–302
   [Google Scholar]
9. Hiller G., Weber K. 1985; Golgi-derived membranes that contain an acylated viral polypeptide are used for vaccinia virus envelopment. Journal of Virology 55:651–659
   [Google Scholar]
10. Hollinshead M., Rodger G., van Eijl H., Hollinshead R., Law M., Vaux D. T., Smith G. L. 2001; Vaccinia virus utilizes microtubules for movement to the cell surface. Journal of Cell Biology 154:389–402
    [Google Scholar]
11. Horton R. M., Cai Z. L., Ho S. N., Pease L. R. 1989; Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques 8:528–535
    [Google Scholar]
12. Hughes S. J., Johnston L. H., de Carlos A., Smith G. L. 1991; Vaccinia virus encodes an active thymidylate kinase that complements a cdc8 mutant of Saccharomyces cerevisiae . Journal of Biological Chemistry 266:20103–20109
    [Google Scholar]
13. Ichihashi Y. 1996; Extracellular enveloped vaccinia virus escapes neutralization. Virology 217:478–485
    [Google Scholar]
14. Ichihashi Y., Dales S. 1971; Biogenesis of poxviruses: interrelationship between haemagglutinin production and polykaryocytosis. Virology 46:533–543
    [Google Scholar]
15. Isaacs S. N., Wolffe E. J., Payne L. G., Moss B. 1992; Characterization of a vaccinia virus-encoded 42-Kilodalton class I membrane glycoprotein component of the extracellular virus envelope. Journal of Virology 66:7217–7224
    [Google Scholar]
16. Katz E., Wolffe E. J., Moss B. 1997; The cytoplasmic and transmembrane domains of the vaccinia virus B5R protein target a chimeric human immunodeficiency virus type 1 glycoprotein to the outer envelope of nascent vaccinia virions. Journal of Virology 71:3178–3187
    [Google Scholar]
17. Law M., Smith G. L. 2001; Antibody neutralization of the extracellular enveloped form of vaccinia virus. Virology 280:132–142
    [Google Scholar]
18. Lorenzo M. M., Herrera E., Blasco R., Isaacs S. N. 1999; Functional analysis of vaccinia virus B5R protein: role of the cytoplasmic tail. Virology 252:450–457
    [Google Scholar]
19. Mackett M., Smith G. L., Moss B. 1985; The construction and characterization of vaccinia virus recombinants expressing foreign genes. In DNA Cloning: a Practical Approach pp 191–211 Edited by Glover D. M. Oxford: IRL Press;
    [Google Scholar]
20. Martinez-Pomares L., Stern R. J., Moyer R. W. 1993; The ps/hr gene (B5R open reading frame homologue) of rabbitpox virus controls pock colour, is a component of extracellular enveloped virus, and is secreted into the medium. Journal of Virology 67:5450–5462
    [Google Scholar]
21. Mathew E., Sanderson C. M., Hollinshead M., Smith G. L. 1998; The extracellular domain of vaccinia virus protein B5R affects plaque phenotype, extracellular enveloped virus release, and intracellular actin tail formation. Journal of Virology 72:2429–2438
    [Google Scholar]
22. Mathew E., Sanderson C. M., Hollinshead R., Hollinshead M., Grimley R., Smith G. L. 1999; The effects of targeting the vaccinia virus B5R protein to the endoplasmic reticulum on virus morphogenesis and dissemination. Virology 265:131–146
    [Google Scholar]
23. Mathew E. C., Sanderson C. M., Hollinshead R., Smith G. L. 2001; A mutational analysis of the vaccinia virus B5R protein. Journal of General Virology 82:1199–1213
    [Google Scholar]
24. Morgan C. 1976; Vaccinia virus reexamined: development and release. Virology 73:43–58
    [Google Scholar]
25. Moss B. 1996; Poxviridae : the viruses and their replication. In Fields Virology pp 2637–2671 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
26. Parkinson J. E., Smith G. L. 1994; Vaccinia virus gene A36R encodes a M_r 43–50 K protein on the surface of extracellular enveloped virus. Virology 204:376–390
    [Google Scholar]
27. Payne L. G., Kristenson K. 1979; Mechanism of vaccinia virus release and its specific inhibition by N ₁-isonicotinoyl- N ₂-3-methyl-4-chlorobenzoylhydrazine. Journal of Virology 32:614–622
    [Google Scholar]
28. Roper R. L., Wolffe E. J., Weisberg A., Moss B. 1998; The envelope protein encoded by the A33R gene is required for formation of actin-containing microvilli and efficient cell-to-cell spread of vaccinia virus. Journal of Virology 72:4192–4204
    [Google Scholar]
29. Röttger S., Frischknecht F., Reckmann I., Smith G. L., Way M. 1999; Interactions between vaccinia virus IEV membrane proteins and their roles in IEV assembly and actin tail formation. Journal of Virology 73:2863–2875
    [Google Scholar]
30. Sanderson C. M., Frischknecht F., Way M., Hollinshead M., Smith G. L. 1998; Roles of vaccinia virus EEV-specific proteins in intracellular actin tail formation and low pH-induced cell–cell fusion. Journal of General Virology 79:1415–1425
    [Google Scholar]
31. Sanderson C. M., Hollinshead M., Smith G. L. 2000; The vaccinia virus A27L gene is needed for the microtubule-dependent transport of intracellular mature virus particles. Journal of General Virology 81:47–58
    [Google Scholar]
32. Schmelz M., Sodeik B., Ericsson M., Wolffe E., Shida H., Hiller G., Griffiths G. 1994; Assembly of vaccinia virus: the second wrapping cisterna is derived from the trans Golgi network. Journal of Virology 68:130–147
    [Google Scholar]
33. Tooze J., Hollinshead M., Reis B., Radsak K., Kern H. 1993; Progeny vaccinia and human cytomegalovirus particles utilize early endosomal cisternae for their envelopes. European Journal of Cell Biology 60:163–178
    [Google Scholar]
34. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76:4350–4354
    [Google Scholar]
35. van Eijl H., Hollinshead M., Smith G. L. 2000; The vaccinia virus A36R protein is a type Ib membrane protein present on intracellular but not extracellular enveloped particles. Virology 271:26–36
    [Google Scholar]
36. van Eijl H., Hollinshead M., Zhang W.-H., Smith G. L. 2002; F12L is associated with intracellular enveloped virus particles and is required for their egress to the cell surface. Journal of General Virology 83:000–000
    [Google Scholar]
37. Ward B. M., Moss B. 2000; Golgi network targeting and plasma membrane internalization signals in vaccinia virus B5R envelope protein. Journal of Virology 74:3771–3780
    [Google Scholar]
38. Ward B. M., Moss B. 2001; Visualization of intracellular movement of vaccinia virus virions containing a green fluorescent protein–B5R membrane protein chimera. Journal of Virology 75:4802–4813
    [Google Scholar]
39. Wolffe E. J., Isaacs S. N., Moss B. 1993; Deletion of the vaccinia virus B5R gene encoding a 42-kiloDalton membrane glycoprotein inhibits extracellular virus envelope formation and dissemination. Journal of Virology 67:4732–4741
    [Google Scholar]
40. Wolffe E. J., Katz E., Weisberg A., Moss B. 1997; The A34R glycoprotein gene is required for induction of specialized actin-containing microvilli and efficient cell-to-cell transmission of vaccinia virus. Journal of Virology 71:3904–3915
    [Google Scholar]
41. Wolffe E. J., Weisberg A. S., Moss B. 1998; Role for the vaccinia virus A36R outer envelope protein in the formation of virus-tipped actin-containing microvilli and cell-to-cell virus spread. Virology 244:20–26
    [Google Scholar]
42. Zhao M., Yang M., Baranov E., Wang X., Penman S., Moossa A. R., Hoffman R. M. 2001; Spatial-temporal imaging of bacterial infection and antibiotic response in intact animals. Proceedings of the National Academy of Sciences, USA 98:9814–9818
    [Google Scholar]