1887

Abstract

A physical map of the genome of granulovirus (AoGV) was constructed for the restriction enzymes HI, II, RI, I and I using restriction endonuclease analysis and DNA hybridization techniques. This enabled the size of the AoGV genome to be estimated at 100·9 kbp. A plasmid library covering 99·9% of the AoGV genome was constructed using five restriction enzymes. The ecdysteroid UDP-glucosyltransferase gene () was located by hybridization with the gene of granulovirus. The sequence of 6000 bp of the region is presented and compared to the equivalent area in other GVs. Database searches showed that this region contained eight open reading frames (ORFs) similar to the baculovirus genes and four ORFs of granulovirus (ORF 178, ORF 2, ORF 7 and ORF 8). The gene was shown to encode an active EGT using an EGT assay. Phylogenetic trees of the granulovirus genes and were constructed using maximum parsimony and distance analyses. These analyses indicated that AoGV genes may be more closely related to other tortricid-infecting GVs than to GVs that infect other lepidopteran families.

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2001-09-01
2020-01-28
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