1887

Abstract

We found previously that mice inoculated orally with simian rotavirus strain RRV developed virus-specific memory B cell responses 16 weeks after immunization that were greater than those found 6 weeks after immunization. Memory B cell responses were defined as the quantity of virus-specific IgA detected in small intestinal lamina propria (LP) fragment cultures of immunized mice at various intervals after challenge. Enhanced memory B cell responses correlated with enhanced protection against shedding. In order to understand better the delayed onset of rotavirus-specific memory B cell responses, a method was developed to determine the frequencies of rotavirus-specific memory B cells in gut-associated lymphoid tissues (GALT). We found that protection against rotavirus challenge was determined by the frequency of rotavirus-specific memory B cells in GALT LP.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-9-2271
2001-09-01
2020-04-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/9/0822271a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-9-2271&mimeType=html&fmt=ahah

References

  1. Arpin C., Dechanet J., Van Kooten C., Merville P., Grouard G., Briere F., Banchereau J., Liu Y. J.. 1995; Generation of memory B cells and plasma cells in vitro. Science268:720–722
    [Google Scholar]
  2. Arpin C., Banchereau J., Liu Y. J.. 1997; Memory B cells are biased towards terminal differentiation: a strategy that may prevent repertoire freezing. Journal of Experimental Medicine186:931–940
    [Google Scholar]
  3. Benedetti R., Lev P., Massouh E., Flo J.. 1998; Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites. Research in Immunology149:107–118
    [Google Scholar]
  4. Coulson B. S., Grimwood K., Hudson I. L., Barnes G. L., Bishop R. F.. 1992; Role of coproantibody in clinical protection of children during reinfection with rotavirus. Journal of Clinical Microbiology30:1678–1684
    [Google Scholar]
  5. Feng N., Burns J. W., Bracy L., Greenberg H. B.. 1994; Comparison of mucosal and systemic humoral immune responses and subsequent protection in mice orally inoculated with a homologous or a heterologous rotavirus. Journal of Virology68:7766–7773
    [Google Scholar]
  6. Franco M. A., Greenberg H. B.. 1995; Role of B cells and cytotoxic T lymphocytes in clearance of and immunity to rotavirus infection in mice. Journal of Virology69:7800–7806
    [Google Scholar]
  7. Gray D., Skarvall H.. 1988; B-cell memory is short-lived in the absence of antigen. Nature336:70–73
    [Google Scholar]
  8. Khoury C. A., Brown K. A., Kim J. E., Offit P. A.. 1994; Rotavirus-specific intestinal immune response in mice assessed by enzyme-linked immunospot assay and intestinal fragment culture. Clinical and Diagnostic Laboratory Immunology1:722–728
    [Google Scholar]
  9. McNeal M. M., Ward R. L.. 1995; Long-term production of rotavirus antibody and protection against reinfection following a single infection of neonatal mice with murine rotavirus. Virology211:474–480
    [Google Scholar]
  10. McNeal M. M., Barone K. S., Rae M. N., Ward R. L.. 1995; Effector functions of antibody and CD8+ cells in resolution of rotavirus infection and protection against reinfection in mice. Virology214:387–397
    [Google Scholar]
  11. Matson D. O., O’Ryan M. L., Herrera I., Pickering L. K., Estes M. K.. 1993; Fecal antibody responses to symptomatic and asymptomatic rotavirus infections. Journal of Infectious Diseases167:577–583
    [Google Scholar]
  12. Moser C. A., Cookinham S., Coffin S. E., Clark H. F., Offit P. A.. 1998; Relative importance of rotavirus-specific effector and memory B cells in protection against challenge. Journal of Virology72:1108–1114
    [Google Scholar]
  13. Offit P. A.. 1996; Host factors associated with protection against rotavirus disease: the skies are clearing. Journal of Infectious Diseases174:S59–S64
    [Google Scholar]
  14. Offit P. A., Clark H. F., Stroop W. G., Twist E. M., Plotkin S. A.. 1983; The cultivation of human rotavirus, strain ‘Wa’, to high titer in cell culture and characterization of the viral structural polypeptides. Journal of Virological Methods7:29–40
    [Google Scholar]
  15. Offit P. A., Cunningham S. L., Dudzik K. I.. 1991; Memory and distribution of virus-specific cytotoxic T lymphocytes (CTLs) and CTL precursors after rotavirus infection. Journal of Virology65:1318–1324
    [Google Scholar]
  16. Ridderstad A., Tarlinton D. M.. 1997; B cell memory in xid mice is long-lived despite reduced memory B cell frequency. Scandinavian Journal of Immunology45:655–659
    [Google Scholar]
  17. Sheridan J. F., Eydelloth R. S., Voderfecht S. L., Aurelian L.. 1983; Virus-specific immunity in neonatal and adult mouse rotavirus infection. Infection and Immunity39:917–927
    [Google Scholar]
  18. Slifka M. K., Ahmed R.. 1996; Limiting dilution analysis of virus-specific memory B cells by an ELISPOT assay. Journal of Immunological Methods199:37–46
    [Google Scholar]
  19. Sprent J.. 1994; T and B memory cells. Cell76:315–322
    [Google Scholar]
  20. Starkey W. G., Collins J., Wallis T. S., Clarke G. J., Spencer A. J., Haddon S. J., Osborne M. P., Candy D. C. A., Stephen J.. 1986; Kinetics, tissue specificity and pathological changes in murine rotavirus infection of mice. Journal of General Virology67:2625–2634
    [Google Scholar]
  21. Ward R. L., McNeal M. M., Sheridan J. F.. 1992; Evidence that active protection following oral immunization of mice with live rotavirus is not dependent on neutralizing antibody. Virology188:57–66
    [Google Scholar]
  22. Williams M. B., Rosé J. R., Rott L. S., Franco M. A., Greenberg H. B., Butcher E. C.. 1998; The memory B cell subset responsible for the secretory IgA response and protective humoral immunity to rotavirus expresses the intestinal homing receptor, α4β7. Journal of Immunology161:4227–4235
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-9-2271
Loading
/content/journal/jgv/10.1099/0022-1317-82-9-2271
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error