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Volume 82, Issue 9

The novel picornavirus contains a strong type II internal ribosomal entry site which functions similarly to that of Free

Abstract

(ERBV) has recently been classified as an , a new genus in the family. ERBV is distantly related to members of the and genera which utilize a type II internal ribosome entry sequence (IRES) to initiate translation. We show that ERBV also possesses the core stem–loop structures (H–L) of a type II IRES. The function of the ERBV IRES was characterized using bicistronic plasmids that were analysed both by transfection into BHK-21 cells and by transcription and translation in rabbit reticulocyte lysates. In both systems, a region encompassed by nucleotides (nt) 189–920 downstream of the poly(C) tract was required for maximal translation. This sequence includes stem–loops H–L as well as four additional upstream stem–loops. Nt 904 corresponds to the second of three in-frame AUG codons located immediately downstream of the polypyrimidine tract (nucleotides 869–880). Site-directed mutagenesis demonstrated that AUG2 is the major initiation codon despite the appropriate positioning of AUG1 16 nt downstream of the polypyrimidine tract. In direct IRES competition experiments, the ERBV IRES was able to compete strongly for translation factors with the IRES of (EMCV). This was true when the assays were performed (with the IRESs competing either in or ) and (with the IRESs competing in ). A comparative analysis of the strength of several IRESs revealed that the ERBV IRES, like that of the EMCV, is a powerful inducer of translation and may have similar potential for use in mammalian expression systems.

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2001-09-01
2024-04-26
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The novel picornavirus Equine rhinitis B virus contains a strong type II internal ribosomal entry site which functions similarly to that of Encephalomyocarditis virus
J. Gen. Virol. 82, 2257 (2001); https://doi.org/10.1099/0022-1317-82-9-2257
/content/journal/jgv/10.1099/0022-1317-82-9-2257
/content/journal/jgv/10.1099/0022-1317-82-9-2257
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