1887

Abstract

The molecular epidemiology of respiratory syncytial virus (RSV) was studied over four consecutive seasons (1997–2000) in a single tertiary hospital in South Africa: 225 isolates were subgrouped by RT–PCR and the resulting products sequenced. Subgroup A predominated in two seasons, while A and B co-circulated approximately equally in the other seasons. The nucleotide sequences of the C-terminal of the G-protein were compared to sequences representative of previously defined RSV genotypes. South African subgroup A and subgroup B isolates clustered into four and five genotypes respectively. One new subgroup A and three new subgroup B genotypes were identified. Different genotypes co-circulated in every season. Different circulation patterns were identified for group A and B isolates. Subgroup A revealed more variability and displacement of genotypes while subgroup B remained more consistent.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-9-2117
2001-09-01
2024-11-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/9/0822117a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-9-2117&mimeType=html&fmt=ahah

References

  1. Anderson L., Hierholzer J., Tsou C., Hendry R., Fernie B., Stone Y., McIntosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  2. Cane P. A. 1997; Analysis of linear epitopes recognised by the primary human antibody response to a variable region of the attachment (G) protein of respiratory syncytial virus. Journal of Medical Virology 51:297–304
    [Google Scholar]
  3. Cane P. A., Pringle C. R. 1992; Molecular epidemiology of respiratory syncytial virus: rapid identification of subgroup A lineages. Journal of Virological Methods 40:297–306
    [Google Scholar]
  4. Cane P., Pringle C. 1995a; Evolution of subgroup A respiratory syncytial virus: evidence for progressive accumulation of amino acid changes in the attachment protein. Journal of Virology 69:2918–2925
    [Google Scholar]
  5. Cane P., Pringle C. 1995b; Molecular epidemiology of respiratory syncytial virus: a review of the use of reverse transcription-polymerase chain reaction in the analysis of genetic variability. Electrophoresis 16:329–333
    [Google Scholar]
  6. Cane P., Matthews D., Pringle C. 1991; Identification of variable domains of the attachment (G) protein of subgroup A respiratory syncytial viruses. Journal of General Virology 72:2091–2096
    [Google Scholar]
  7. Cane P. A., Matthews D. A., Pringle C. R. 1994; Analysis of respiratory syncytial virus strain variation in successive epidemics in one city. Journal of Clinical Microbiology 32:1–4
    [Google Scholar]
  8. Cane P. A., Weber M., Sanneh M., Dackour R., Pringle C. R., Whittle H. 1999; Molecular epidemiology of respiratory syncytial virus in The Gambia. Epidemiology and Infection 122:155–160
    [Google Scholar]
  9. Choi E., Lee H. 2000; Genetic diversity and molecular epidemiology of the G protein of subgroups A and B of respiratory syncytial viruses isolated over 9 consecutive epidemics in Korea. Journal of Infectious Diseases 181:1547–1556
    [Google Scholar]
  10. Coggins W. B., Lefkowitz E. J., Sullender W. M. 1998; Genetic variability among group A and group B respiratory syncytial viruses in a children’s hospital. Journal of Clinical Microbiology 36:3552–3557
    [Google Scholar]
  11. Felsenstein J. 1993 PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author Department of Genetics, University of Washington, Seattle. Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  12. Garcia O., Martin M., Dopazo J., Arbiza J., Frabasile S., Russi J., Hortal M., Perez-Brena P., Martinez I., Garcia-Barreno B. and others 1994; Evolutionary pattern of human respiratory syncytial virus (subgroup A): cocirculating lineages and correlation of genetic and antigenic changes in the G glycoprotein. Journal of Virology 68:5448–5459
    [Google Scholar]
  13. Hall C. B., Walsh E. E., Schnabel K. C., Long C. E., McConnochie K. M., Hildreth S. W., Anderson L. J. 1990; Occurrence of groups A and B of respiratory syncytial virus over 15 years: associated epidemiologic and clinical characteristics in hospitalized and ambulatory children. Journal of Infectious Diseases 162:1283–1290
    [Google Scholar]
  14. Johnson P. J., Olmsted R., Prince G., Murphy B., Alling D., Walsh E., Collins P. 1987; Antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of F and G glycoproteins to immunity. Journal of Virology 61:3163–3166
    [Google Scholar]
  15. Karron R. A., Buonagurio D. A., Georgiu A. F., Whitehead S. S., Adamus J. E., Clements-Mann M. L., Harris D. O., Randolph V. B., Udem S. A., Murphy B. R., Sidhu M. S. 1997; Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant. Proceedings of the National Academy of Sciences, USA 94:13961–13966
    [Google Scholar]
  16. Kumar S., Tamura K., Jakobsen I., Nei M. 2001; MEGA2: molecular evolutionary genetics analyses software. Version 2:0 University Park: Pennsylvania State University;
    [Google Scholar]
  17. Lepage P., Spira R., Kalibala S., Pillay K., Giaquinto C., Castetbon K., Osborne C., Courpotin C., Dabis F. 1998; Care of human immunodeficiency virus-infected children in developing countries. International Working Group on Mother-to-Child Transmission of HIV.. Pediatric Infectious Disease Journal 17:581–586
    [Google Scholar]
  18. Madhi S., Venter M., Madhi A., Petersen K., Klugman H. 2001; Differing manifestations of respiratory syncytial virus-associated severe lower respiratory tract infections in human immunodeficiency virus type 1-infected and uninfected children. Pediatric Infectious Disease Journal 20:164–170
    [Google Scholar]
  19. Martinez I., Valdes O., Delfraro A., Arbiza J., Russi J., Melero J. A. 1999; Evolutionary pattern of the G glycoprotein of human respiratory syncytial viruses from antigenic group B: the use of alternative termination codons and lineage diversification. Journal of General Virology 80:125–130
    [Google Scholar]
  20. Melero J. A., Garcia-Barreno B., Martinez I., Pringle C. R., Cane P. A. 1997; Antigenic structure, evolution and immunobiology of human respiratory syncytial virus attachment (G) protein. Journal of General Virology 78:2411–2418
    [Google Scholar]
  21. Mufson M., Belshe R., Orvell C., Norrby E. 1988; Respiratory syncytial virus epidemics: variable dominance of subgroups A and B strains among children, 1981–1986. Journal of Infectious Diseases 157:143–148
    [Google Scholar]
  22. Peret T. C., Hall C. B., Schnabel K. C., Golub J. A., Anderson L. J. 1998; Circulation patterns of genetically distinct group A and B strains of human respiratory syncytial virus in a community. Journal of General Virology 79:2221–2229
    [Google Scholar]
  23. Peret T. C., Hall C. B., Hammond G. W., Piedra P. A., Storch G. A., Sullender W. M., Tsou C., Anderson L. J. 2000; Circulation patterns of group A and B human respiratory syncytial virus genotypes in 5 communities in North America. Journal of Infectious Diseases 181:1891–1896
    [Google Scholar]
  24. Roca A., Loscertales M. P., Quinto L., Perez-Brena P., Vaz N., Alonso P. L., Saiz J. C. 2001; Genetic variability among group A and B respiratory syncytial viruses in Mozambique: identification of a new cluster of group B isolates. Journal of General Virology 82:103–111
    [Google Scholar]
  25. Selwyn B. 1990; The epidemiology of acute respiratory tract infection in young children: comparison of findings from several developing countries. Reviews in Infectious Diseases 12:870–888
    [Google Scholar]
  26. Strimmer K., von Haeseler A. 1996; Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Molecular Biology and Evolution 13:964–969
    [Google Scholar]
  27. Sullender W. 2000; Respiratory syncytial virus genetic and antigenic diversity. Clinical Microbiology Reviews Jan. 1–15
    [Google Scholar]
  28. Sullender W. M., Anderson K., Wertz G. W. 1990; The respiratory syncytial virus subgroup B attachment glycoprotein: analysis of sequence, expression from a recombinant vector, and evaluation as an immunogen against homologous and heterologous subgroup virus challenge. Virology 178:195–203
    [Google Scholar]
  29. Sullender W. M., Mufson M. A., Anderson L. J., Wertz G. W. 1991; Genetic diversity of the attachment protein of subgroup B respiratory syncytial viruses. Journal of Virology 65:5425–5434
    [Google Scholar]
  30. Sullender W. M., Sun L., Anderson L. J. 1993; Analysis of respiratory syncytial virus genetic variability with amplified cDNAs. Journal of Clinical Microbiology 31:1224–1231
    [Google Scholar]
  31. Sullender W. M., Mufson M. A., Prince G. A., Anderson L. J., Wertz G. W. 1998; Antigenic and genetic diversity among the attachment proteins of group A respiratory syncytial viruses that have caused repeat infections in children. Journal of Infectious Diseases 178:925–932
    [Google Scholar]
  32. Wertz G. W., Collins P. L., Huang Y., Gruber C., Levine S., Ball L. A. 1985; Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Academy of Sciences, USA 82:4075–4079
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-82-9-2117
Loading
/content/journal/jgv/10.1099/0022-1317-82-9-2117
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error