1887

Abstract

The molecular epidemiology of respiratory syncytial virus (RSV) was studied over four consecutive seasons (1997–2000) in a single tertiary hospital in South Africa: 225 isolates were subgrouped by RT–PCR and the resulting products sequenced. Subgroup A predominated in two seasons, while A and B co-circulated approximately equally in the other seasons. The nucleotide sequences of the C-terminal of the G-protein were compared to sequences representative of previously defined RSV genotypes. South African subgroup A and subgroup B isolates clustered into four and five genotypes respectively. One new subgroup A and three new subgroup B genotypes were identified. Different genotypes co-circulated in every season. Different circulation patterns were identified for group A and B isolates. Subgroup A revealed more variability and displacement of genotypes while subgroup B remained more consistent.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-9-2117
2001-09-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/9/0822117a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-9-2117&mimeType=html&fmt=ahah

References

  1. Anderson, L., Hierholzer, J., Tsou, C., Hendry, R., Fernie, B., Stone, Y. & McIntosh, K. ( 1985; ). Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151, 626-633.[CrossRef]
    [Google Scholar]
  2. Cane, P. A. ( 1997; ). Analysis of linear epitopes recognised by the primary human antibody response to a variable region of the attachment (G) protein of respiratory syncytial virus. Journal of Medical Virology 51, 297-304.[CrossRef]
    [Google Scholar]
  3. Cane, P. A. & Pringle, C. R. ( 1992; ). Molecular epidemiology of respiratory syncytial virus: rapid identification of subgroup A lineages. Journal of Virological Methods 40, 297-306.[CrossRef]
    [Google Scholar]
  4. Cane, P. & Pringle, C. ( 1995a; ). Evolution of subgroup A respiratory syncytial virus: evidence for progressive accumulation of amino acid changes in the attachment protein. Journal of Virology 69, 2918-2925.
    [Google Scholar]
  5. Cane, P. & Pringle, C. ( 1995b; ). Molecular epidemiology of respiratory syncytial virus: a review of the use of reverse transcription-polymerase chain reaction in the analysis of genetic variability. Electrophoresis 16, 329-333.[CrossRef]
    [Google Scholar]
  6. Cane, P., Matthews, D. & Pringle, C. ( 1991; ). Identification of variable domains of the attachment (G) protein of subgroup A respiratory syncytial viruses. Journal of General Virology 72, 2091-2096.[CrossRef]
    [Google Scholar]
  7. Cane, P. A., Matthews, D. A. & Pringle, C. R. ( 1994; ). Analysis of respiratory syncytial virus strain variation in successive epidemics in one city. Journal of Clinical Microbiology 32, 1-4.
    [Google Scholar]
  8. Cane, P. A., Weber, M., Sanneh, M., Dackour, R., Pringle, C. R. & Whittle, H. ( 1999; ). Molecular epidemiology of respiratory syncytial virus in The Gambia. Epidemiology and Infection 122, 155-160.[CrossRef]
    [Google Scholar]
  9. Choi, E. & Lee, H. ( 2000; ). Genetic diversity and molecular epidemiology of the G protein of subgroups A and B of respiratory syncytial viruses isolated over 9 consecutive epidemics in Korea. Journal of Infectious Diseases 181, 1547-1556.[CrossRef]
    [Google Scholar]
  10. Coggins, W. B., Lefkowitz, E. J. & Sullender, W. M. ( 1998; ). Genetic variability among group A and group B respiratory syncytial viruses in a children’s hospital. Journal of Clinical Microbiology 36, 3552-3557.
    [Google Scholar]
  11. Felsenstein, J. (1993). PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle. Department of Genetics, University of Washington, Seattle, WA, USA.
  12. Garcia, O., Martin, M., Dopazo, J., Arbiza, J., Frabasile, S., Russi, J., Hortal, M., Perez-Brena, P., Martinez, I., Garcia-Barreno, B. and others ( 1994; ). Evolutionary pattern of human respiratory syncytial virus (subgroup A): cocirculating lineages and correlation of genetic and antigenic changes in the G glycoprotein. Journal of Virology 68, 5448–5459.
    [Google Scholar]
  13. Hall, C. B., Walsh, E. E., Schnabel, K. C., Long, C. E., McConnochie, K. M., Hildreth, S. W. & Anderson, L. J. ( 1990; ). Occurrence of groups A and B of respiratory syncytial virus over 15 years: associated epidemiologic and clinical characteristics in hospitalized and ambulatory children. Journal of Infectious Diseases 162, 1283-1290.[CrossRef]
    [Google Scholar]
  14. Johnson, P. J., Olmsted, R., Prince, G., Murphy, B., Alling, D., Walsh, E. & Collins, P. ( 1987; ). Antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of F and G glycoproteins to immunity. Journal of Virology 61, 3163-3166.
    [Google Scholar]
  15. Karron, R. A., Buonagurio, D. A., Georgiu, A. F., Whitehead, S. S., Adamus, J. E., Clements-Mann, M. L., Harris, D. O., Randolph, V. B., Udem, S. A., Murphy, B. R. & Sidhu, M. S. ( 1997; ). Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro: clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant. Proceedings of the National Academy of Sciences, USA 94, 13961-13966.[CrossRef]
    [Google Scholar]
  16. Kumar, S., Tamura, K., Jakobsen, I. & Nei, M. (2001). MEGA2: molecular evolutionary genetics analyses software. Version 2.0. University Park: Pennsylvania State University.
  17. Lepage, P., Spira, R., Kalibala, S., Pillay, K., Giaquinto, C., Castetbon, K., Osborne, C., Courpotin, C. & Dabis, F. ( 1998; ). Care of human immunodeficiency virus-infected children in developing countries. International Working Group on Mother-to-Child Transmission of HIV. Pediatric Infectious Disease Journal 17, 581-586.[CrossRef]
    [Google Scholar]
  18. Madhi, S., Venter, M., Madhi, A., Petersen, K. & Klugman, H. ( 2001; ). Differing manifestations of respiratory syncytial virus-associated severe lower respiratory tract infections in human immunodeficiency virus type 1-infected and uninfected children. Pediatric Infectious Disease Journal 20, 164-170.[CrossRef]
    [Google Scholar]
  19. Martinez, I., Valdes, O., Delfraro, A., Arbiza, J., Russi, J. & Melero, J. A. ( 1999; ). Evolutionary pattern of the G glycoprotein of human respiratory syncytial viruses from antigenic group B: the use of alternative termination codons and lineage diversification. Journal of General Virology 80, 125-130.
    [Google Scholar]
  20. Melero, J. A., Garcia-Barreno, B., Martinez, I., Pringle, C. R. & Cane, P. A. ( 1997; ). Antigenic structure, evolution and immunobiology of human respiratory syncytial virus attachment (G) protein. Journal of General Virology 78, 2411-2418.
    [Google Scholar]
  21. Mufson, M., Belshe, R., Orvell, C. & Norrby, E. ( 1988; ). Respiratory syncytial virus epidemics: variable dominance of subgroups A and B strains among children, 1981–1986. Journal of Infectious Diseases 157, 143-148.[CrossRef]
    [Google Scholar]
  22. Peret, T. C., Hall, C. B., Schnabel, K. C., Golub, J. A. & Anderson, L. J. ( 1998; ). Circulation patterns of genetically distinct group A and B strains of human respiratory syncytial virus in a community. Journal of General Virology 79, 2221-2229.
    [Google Scholar]
  23. Peret, T. C., Hall, C. B., Hammond, G. W., Piedra, P. A., Storch, G. A., Sullender, W. M., Tsou, C. & Anderson, L. J. ( 2000; ). Circulation patterns of group A and B human respiratory syncytial virus genotypes in 5 communities in North America. Journal of Infectious Diseases 181, 1891-1896.[CrossRef]
    [Google Scholar]
  24. Roca, A., Loscertales, M. P., Quinto, L., Perez-Brena, P., Vaz, N., Alonso, P. L. & Saiz, J. C. ( 2001; ). Genetic variability among group A and B respiratory syncytial viruses in Mozambique: identification of a new cluster of group B isolates. Journal of General Virology 82, 103-111.
    [Google Scholar]
  25. Selwyn, B. ( 1990; ). The epidemiology of acute respiratory tract infection in young children: comparison of findings from several developing countries. Reviews in Infectious Diseases 12, 870-888.[CrossRef]
    [Google Scholar]
  26. Strimmer, K. & von Haeseler, A. ( 1996; ). Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Molecular Biology and Evolution 13, 964-969.[CrossRef]
    [Google Scholar]
  27. Sullender, W. (2000). Respiratory syncytial virus genetic and antigenic diversity. Clinical Microbiology Reviews, Jan., 1-15.
  28. Sullender, W. M., Anderson, K. & Wertz, G. W. ( 1990; ). The respiratory syncytial virus subgroup B attachment glycoprotein: analysis of sequence, expression from a recombinant vector, and evaluation as an immunogen against homologous and heterologous subgroup virus challenge. Virology 178, 195-203.[CrossRef]
    [Google Scholar]
  29. Sullender, W. M., Mufson, M. A., Anderson, L. J. & Wertz, G. W. ( 1991; ). Genetic diversity of the attachment protein of subgroup B respiratory syncytial viruses. Journal of Virology 65, 5425-5434.
    [Google Scholar]
  30. Sullender, W. M., Sun, L. & Anderson, L. J. ( 1993; ). Analysis of respiratory syncytial virus genetic variability with amplified cDNAs. Journal of Clinical Microbiology 31, 1224-1231.
    [Google Scholar]
  31. Sullender, W. M., Mufson, M. A., Prince, G. A., Anderson, L. J. & Wertz, G. W. ( 1998; ). Antigenic and genetic diversity among the attachment proteins of group A respiratory syncytial viruses that have caused repeat infections in children. Journal of Infectious Diseases 178, 925-932.[CrossRef]
    [Google Scholar]
  32. Wertz, G. W., Collins, P. L., Huang, Y., Gruber, C., Levine, S. & Ball, L. A. ( 1985; ). Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Academy of Sciences, USA 82, 4075-4079.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-9-2117
Loading
/content/journal/jgv/10.1099/0022-1317-82-9-2117
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error