1887

Abstract

Using a cDNA expression cassette in combination with agroinoculation of potato leaf discs we have investigated the role the protein encoded by ORF0 of (PLRV) and have shown its importance for virus accumulation. Two mutations introduced into ORF0 by site-directed mutagenesis prevented expression of the corresponding protein and completely abolished virus accumulation in plant cells. They did not, however, affect translation of ORF1 and ORF2. We therefore conclude that ORF0 of PLRV produces a protein essential for virus accumulation, a hitherto undescribed finding.

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2001-06-01
2019-10-14
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References

  1. Amberg, S. M., Nestorowicz, A., McCourt, D. W. & Rice, C. M. ( 1994; ). NS2B3 proteinase-mediated processing in the yellow fever virus structural region: in vitro and in vivo studies. Journal of Virology 68, 3794-3802.
    [Google Scholar]
  2. Arias, C. F., Preugschat, F. & Strauss, J. H. ( 1993; ). Dengue 2 virus NS2B and NS3 form a stable complex that can cleave NS3 within the helicase domain. Virology 193, 888-899.[CrossRef]
    [Google Scholar]
  3. Bevan, M. ( 1984; ). Binary Agrobacterium vectors for plant transformation. Nucleic Acids Research 12, 8711-8721.[CrossRef]
    [Google Scholar]
  4. Bonneau, C., Brugidou, C., Chen, L., Beachy, R. N. & Fauquet, C. ( 1998; ). Expression of the rice yellow mottle virus P1 protein in vitro and in vivo and its involvement in virus spread. Virology 244, 79-86.[CrossRef]
    [Google Scholar]
  5. Boyer, J.-Ch. & Haenni, A.-L. ( 1994; ). Infectious transcripts and cDNA clones of RNA viruses. Virology 198, 415-426.[CrossRef]
    [Google Scholar]
  6. Datta, U. & Dasgupta, A. ( 1994; ). Expression and subcellular localization of poliovirus VPg-precursor protein 3AB in eukaryotic cells: evidence for glycosylation in vitro. Journal of Virology 68, 4468-4477.
    [Google Scholar]
  7. de Groot, R. J., Hardy, W. R., Shirako, Y. & Strauss, J. H. ( 1990; ). Cleavage-site preferences of Sindbis virus polyproteins containing the non-structural proteinase. Evidence for temporal regulation of polyprotein processing in vivo. EMBO Journal 9, 2631-2638.
    [Google Scholar]
  8. Hulanicka, M. D., Sadowy, E., Juszczuk, M. & Gronenborn, B. (1999). ORF1 of potato leafroll virus encodes a serine proteinase indispensable for viral replication. Oral presentation at the XIth International Congress of Virology, Sydney, 9–13 August 1999.
  9. Kujawa, A. B., Drugeon, G., Hulanicka, D. & Haenni, A.-L. ( 1993; ). Structural requirements for efficient translational frameshifting in the synthesis of the putative viral RNA-dependent RNA polymerase of potato leafroll virus. Nucleic Acids Research 21, 2165-2171.[CrossRef]
    [Google Scholar]
  10. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685.[CrossRef]
    [Google Scholar]
  11. Lama, J., Paul, A. V., Harris, K. S. & Wimmer, E. ( 1994; ). Properties of purified recombinant poliovirus protein 3AB as substrate for viral proteinases and as co-factor for RNA polymerase 3Dpol. Journal of Biological Chemistry 269, 66-70.
    [Google Scholar]
  12. Mayo, M. A. & Ziegler-Graff, V. ( 1996; ). Molecular biology of luteoviruses. Advances in Virus Research 46, 416-460.
    [Google Scholar]
  13. Mayo, M. A., Barker, H., Robinson, D. J., Tamada, T. & Harrison, B. D. ( 1982; ). Evidence that potato leafroll virus RNA is positive-stranded, is linked to a small protein and does not contain polyadenylate. Journal of General Virology 59, 163-167.[CrossRef]
    [Google Scholar]
  14. Mayo, M. A., Robinson, D. J., Jolly, C. A. & Hyman, L. ( 1989; ). Nucleotide sequence of potato leafroll luteovirus RNA. Journal of General Virology 70, 1037-1051.[CrossRef]
    [Google Scholar]
  15. Miller, W. A., Brown, C. M. & Wang, S. ( 1997; ). New punctuation for the genetic code: luteovirus gene expression. Seminars in Virology 8, 3-13.[CrossRef]
    [Google Scholar]
  16. Molla, A., Harris, K. S., Paul, A. V., Shin, S. H., Mugevero, J. & Wimmer, E. ( 1994; ). Stimulation of poliovirus proteinase 3Cpro-related proteolysis by the genome-linked protein VPg and its precursor 3AB. Journal of Biological Chemistry 269, 27015-27020.
    [Google Scholar]
  17. Pringle, C. R. ( 1998; ). Virus taxonomy – San Diego 1998. Report of the 27th Meeting of the Executive Committee of the International Committee on Taxonomy of Viruses. Archives of Virology 143, 1449-1459.[CrossRef]
    [Google Scholar]
  18. Reutenauer, A., Ziegler-Graff, V., Lot, H., Scheidecker, D., Guilley, H., Richards, K. & Jonard, G. ( 1993; ). Identification of beet western yellows luteovirus genes implicated in viral replication and particle morphogenesis. Virology 195, 692-699.[CrossRef]
    [Google Scholar]
  19. Richards, O. C. & Ehrenfeld, E. ( 1998; ). Effects of poliovirus 3AB protein on 3D polymerase-catalyzed reaction. Journal of Biological Chemistry 273, 12832-12840.[CrossRef]
    [Google Scholar]
  20. Sadowy, E., Pluta, K., Gronenborn, B. & Hulanicka, D. ( 1998; ). Infectious transcripts from cloned cDNA of potato leafroll virus. Acta Biochimica Polonica 45, 611-619.
    [Google Scholar]
  21. Sadowy, E., Wiśniewska, A., Gronenborn, B. & Hulanicka, D. (2000). Genes involved in Potato leafroll virus replication. Plant Virus Invasion and Host Defence. EMBO Workshop, Kolymbari, Crete, Greece, 28 May–1 June 2000.
  22. Sadowy, E., Juszczuk, M., David, C., Gronenborn, B. & Hulanicka, M. D. ( 2001; ). Mutational analysis of the proteinase function of Potato leafroll virus. Journal of General Virology 82, 1517-1527.
    [Google Scholar]
  23. Schaad, M. C., Jensen, P. E. & Carrington, J. C. ( 1997; ). Formation of plant RNA virus replication complexes on membranes: role of an endoplasmic reticulum-targeted viral protein. EMBO Journal 16, 4049-4059.[CrossRef]
    [Google Scholar]
  24. Tamm, T. & Truve, E. ( 2000a; ). Sobemoviruses. Journal of Virology 74, 6231-6241.[CrossRef]
    [Google Scholar]
  25. Tamm, T. & Truve, E. ( 2000b; ). RNA-binding activities of cocksfoot mottle sobemovirus proteins. Virus Research 66, 197-207.[CrossRef]
    [Google Scholar]
  26. Towner, J. S., Ho, T. V. & Semler, B. L. ( 1996; ). Determinants of membrane association for poliovirus protein 3AB. Journal of Biological Chemistry 271, 26810-26818.[CrossRef]
    [Google Scholar]
  27. van der Wilk, F., Houterman, P., Molthoff, J., Hans, F., Dekker, B., van den Heuvel, J. M. F. M., Huttinga, H. & Goldbach, R. ( 1997; ). Expression of the potato leafroll virus ORF0 induces viral-disease-like symptoms in transgenic potato plants. Molecular Plant–Microbe Interactions 10, 153-159.[CrossRef]
    [Google Scholar]
  28. Voinnet, O., Pinto, Y. M. & Baulcombe, D. C. ( 1999; ). Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proceedings of National Academy of Sciences, USA 96, 14147-14152.[CrossRef]
    [Google Scholar]
  29. Wassenaar, A. L. M., Spaan, W. J. M., Gorbalenya, A. E. & Snijder, E. J. ( 1997; ). Alternative proteolytic processing of the arterivirus replicase ORF1a polyprotein: evidence that NSP2 acts as a cofactor for the NSP4 serine protease. Journal of Virology 71, 9313-9322.
    [Google Scholar]
  30. Webster, A., Leith, I. R. & Hay, R. T. ( 1994; ). Activation of adenovirus-coded protease and processing of preterminal protein. Journal of Virology 68, 7292-7300.
    [Google Scholar]
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